De Novo Synthesis of Basal Bacterial Cell Division Proteins FtsZ, FtsA, and ZipA Inside Giant Vesicles

Takumi Furusato, Fumihiro Horie, Hideaki T. Matsubayashi, Kazuaki Amikura, Yutetsu Kuruma, Takuya Ueda

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Cell division is the most dynamic event in the cell cycle. Recently, efforts have been made to reconstruct it using the individual component proteins to obtain a better understanding of the process of self-reproduction of cells. However, such reconstruction studies are frequently hampered by difficulties in preparing membrane-associated proteins. Here we demonstrate a de novo synthesis approach based on a cell-free translation system. Genes for fundamental cell division proteins, FtsZ, FtsA, and ZipA, were expressed inside the lipid compartment of giant vesicles (GVs). The synthesized proteins showed polymerization, membrane localization, and eventually membrane deformation. Notably, we found that this morphological change of the vesicle is forced by only FtsZ and ZipA, which form clusters on the membrane at the vesicle interior. Our cell-free approach provides a platform for studying protein dynamics associated with lipid membrane and paves the way to create a synthetic cell that undergoes self-reproduction.

Original languageEnglish
Pages (from-to)953-961
Number of pages9
JournalACS Synthetic Biology
Volume7
Issue number4
DOIs
Publication statusPublished - 2018 Apr 20
Externally publishedYes

Fingerprint

Cell Division
Cells
Proteins
Membranes
Reproduction
Artificial Cells
Cell-Free System
Membrane Lipids
Polymerization
Cell Cycle
Membrane Proteins
Genes
Lipids

Keywords

  • cell division
  • Cell-free translation
  • FtsZ
  • giant vesicles
  • PURE system
  • synthetic cell

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

De Novo Synthesis of Basal Bacterial Cell Division Proteins FtsZ, FtsA, and ZipA Inside Giant Vesicles. / Furusato, Takumi; Horie, Fumihiro; Matsubayashi, Hideaki T.; Amikura, Kazuaki; Kuruma, Yutetsu; Ueda, Takuya.

In: ACS Synthetic Biology, Vol. 7, No. 4, 20.04.2018, p. 953-961.

Research output: Contribution to journalArticle

Furusato, Takumi ; Horie, Fumihiro ; Matsubayashi, Hideaki T. ; Amikura, Kazuaki ; Kuruma, Yutetsu ; Ueda, Takuya. / De Novo Synthesis of Basal Bacterial Cell Division Proteins FtsZ, FtsA, and ZipA Inside Giant Vesicles. In: ACS Synthetic Biology. 2018 ; Vol. 7, No. 4. pp. 953-961.
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