Deciphering the relationship among phosphate dynamics, electron-dense body and lipid accumulation in the green alga Parachlorella kessleri

Shuhei Ota, Mai Yoshihara, Tomokazu Yamazaki, Tsuyoshi Takeshita, Aiko Hirata, Mami Konomi, Kenshiro Oshima, Masahira Hattori, Katerina Bišová, Vilém Zachleder, Shigeyuki Kawano

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Abstract

Phosphorus is an essential element for life on earth and is also important for modern agriculture, which is dependent on inorganic fertilizers from phosphate rock. Polyphosphate is a biological polymer of phosphate residues, which is accumulated in organisms during the biological wastewater treatment process to enhance biological phosphorus removal. Here, we investigated the relationship between polyphosphate accumulation and electron-dense bodies in the green alga Parachlorella kessleri. Under sulfur-depleted conditions, in which some symporter genes were upregulated, while others were downregulated, total phosphate accumulation increased in the early stage of culture compared to that under sulfur-replete conditions. The P signal was detected only in dense bodies by energy dispersive X-ray analysis. Transmission electron microscopy revealed marked ultrastructural variations in dense bodies with and without polyphosphate. Our findings suggest that the dense body is a site of polyphosphate accumulation, and P. kessleri has potential as a phosphate-accumulating organism.

Original languageEnglish
Article number25731
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016 May 16
Externally publishedYes

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Ota, S., Yoshihara, M., Yamazaki, T., Takeshita, T., Hirata, A., Konomi, M., Oshima, K., Hattori, M., Bišová, K., Zachleder, V., & Kawano, S. (2016). Deciphering the relationship among phosphate dynamics, electron-dense body and lipid accumulation in the green alga Parachlorella kessleri. Scientific Reports, 6, [25731]. https://doi.org/10.1038/srep25731