Decrease in the efficiency of the electron donation to tyrosine Z of photosystem II in an SQDG-deficient mutant of Chlamydomonas

Ayumi Minoda; Kintake Sonoike; Katsuhiko Okada; Norihiro Sato; Mikio Tsuzuki

doi:10.1016/S0014-5793(03)00981-5

Decrease in the efficiency of the electron donation to tyrosine Z of photosystem II in an SQDG-deficient mutant of Chlamydomonas

Ayumi Minoda, Kintake Sonoike, Katsuhiko Okada, Norihiro Sato, Mikio Tsuzuki

Research output: Contribution to journal › Article › peer-review

49 Citations (Scopus)

Abstract

Photosystem (PS) II activity of a sulfoquinovosyl diacylglycerol (SQDG)-deficient mutant (hf-2) of Chlamydomonas was partially decreased compared with that of wild-type. The susceptibility to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was also modified in the mutant. Photometric measurements in the isolated thylakoid membranes of hf-2 revealed that the lowered activity in the mutant was derived from a decrease in the efficiency of the electron donation from water to tyrosine Z, not from the efficiency of the electron transport from Q_A to Q_B. This result was confirmed by the decay kinetics of chlorophyll fluorescence determined in vivo. We conclude that SQDG contributes to maintaining the conformation of PSII complexes, particularly that of D1 polypeptides, which are necessary for maximum activities in Chlamydomonas.

Original language	English
Pages (from-to)	109-112
Number of pages	4
Journal	FEBS Letters
Volume	553
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-5793(03)00981-5
Publication status	Published - 2003 Oct 9
Externally published	Yes

Keywords

Chlamydomonas
Glycolipid
Photosystem II
Sulfoquinovosyl diacylglycerol
Thylakoid membrane

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(03)00981-5

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title = "Decrease in the efficiency of the electron donation to tyrosine Z of photosystem II in an SQDG-deficient mutant of Chlamydomonas",

abstract = "Photosystem (PS) II activity of a sulfoquinovosyl diacylglycerol (SQDG)-deficient mutant (hf-2) of Chlamydomonas was partially decreased compared with that of wild-type. The susceptibility to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was also modified in the mutant. Photometric measurements in the isolated thylakoid membranes of hf-2 revealed that the lowered activity in the mutant was derived from a decrease in the efficiency of the electron donation from water to tyrosine Z, not from the efficiency of the electron transport from QA to QB. This result was confirmed by the decay kinetics of chlorophyll fluorescence determined in vivo. We conclude that SQDG contributes to maintaining the conformation of PSII complexes, particularly that of D1 polypeptides, which are necessary for maximum activities in Chlamydomonas.",

keywords = "Chlamydomonas, Glycolipid, Photosystem II, Sulfoquinovosyl diacylglycerol, Thylakoid membrane",

author = "Ayumi Minoda and Kintake Sonoike and Katsuhiko Okada and Norihiro Sato and Mikio Tsuzuki",

note = "Funding Information: The authors thank Dr. T. Noguchi of the University of Tsukuba for his kind help during research. This work was supported by grants from the Ministry of Education, Science, Sports and Culture, from the Promotion and Mutual Aid Corporation for Private Schools of Japan, and from CREST of JST (Japan).",

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doi = "10.1016/S0014-5793(03)00981-5",

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T1 - Decrease in the efficiency of the electron donation to tyrosine Z of photosystem II in an SQDG-deficient mutant of Chlamydomonas

AU - Minoda, Ayumi

AU - Sonoike, Kintake

AU - Okada, Katsuhiko

AU - Sato, Norihiro

AU - Tsuzuki, Mikio

N1 - Funding Information: The authors thank Dr. T. Noguchi of the University of Tsukuba for his kind help during research. This work was supported by grants from the Ministry of Education, Science, Sports and Culture, from the Promotion and Mutual Aid Corporation for Private Schools of Japan, and from CREST of JST (Japan).

PY - 2003/10/9

Y1 - 2003/10/9

N2 - Photosystem (PS) II activity of a sulfoquinovosyl diacylglycerol (SQDG)-deficient mutant (hf-2) of Chlamydomonas was partially decreased compared with that of wild-type. The susceptibility to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was also modified in the mutant. Photometric measurements in the isolated thylakoid membranes of hf-2 revealed that the lowered activity in the mutant was derived from a decrease in the efficiency of the electron donation from water to tyrosine Z, not from the efficiency of the electron transport from QA to QB. This result was confirmed by the decay kinetics of chlorophyll fluorescence determined in vivo. We conclude that SQDG contributes to maintaining the conformation of PSII complexes, particularly that of D1 polypeptides, which are necessary for maximum activities in Chlamydomonas.

AB - Photosystem (PS) II activity of a sulfoquinovosyl diacylglycerol (SQDG)-deficient mutant (hf-2) of Chlamydomonas was partially decreased compared with that of wild-type. The susceptibility to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was also modified in the mutant. Photometric measurements in the isolated thylakoid membranes of hf-2 revealed that the lowered activity in the mutant was derived from a decrease in the efficiency of the electron donation from water to tyrosine Z, not from the efficiency of the electron transport from QA to QB. This result was confirmed by the decay kinetics of chlorophyll fluorescence determined in vivo. We conclude that SQDG contributes to maintaining the conformation of PSII complexes, particularly that of D1 polypeptides, which are necessary for maximum activities in Chlamydomonas.

KW - Chlamydomonas

KW - Glycolipid

KW - Photosystem II

KW - Sulfoquinovosyl diacylglycerol

KW - Thylakoid membrane

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Decrease in the efficiency of the electron donation to tyrosine Z of photosystem II in an SQDG-deficient mutant of Chlamydomonas

Abstract

Keywords

ASJC Scopus subject areas

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