Decrease in the efficiency of the electron donation to tyrosine Z of photosystem II in an SQDG-deficient mutant of Chlamydomonas

Ayumi Minoda, Kintake Sonoike, Katsuhiko Okada, Norihiro Sato, Mikio Tsuzuki

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Photosystem (PS) II activity of a sulfoquinovosyl diacylglycerol (SQDG)-deficient mutant (hf-2) of Chlamydomonas was partially decreased compared with that of wild-type. The susceptibility to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was also modified in the mutant. Photometric measurements in the isolated thylakoid membranes of hf-2 revealed that the lowered activity in the mutant was derived from a decrease in the efficiency of the electron donation from water to tyrosine Z, not from the efficiency of the electron transport from QA to QB. This result was confirmed by the decay kinetics of chlorophyll fluorescence determined in vivo. We conclude that SQDG contributes to maintaining the conformation of PSII complexes, particularly that of D1 polypeptides, which are necessary for maximum activities in Chlamydomonas.

Original languageEnglish
Pages (from-to)109-112
Number of pages4
JournalFEBS Letters
Volume553
Issue number1-2
DOIs
Publication statusPublished - 2003 Oct 9
Externally publishedYes

Fingerprint

Diuron
Chlamydomonas
Photosystem II Protein Complex
Diglycerides
Tyrosine
Electrons
Thylakoids
Chlorophyll
Electron Transport
Conformations
Fluorescence
Membranes
Peptides
Kinetics
Water

Keywords

  • Chlamydomonas
  • Glycolipid
  • Photosystem II
  • Sulfoquinovosyl diacylglycerol
  • Thylakoid membrane

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Decrease in the efficiency of the electron donation to tyrosine Z of photosystem II in an SQDG-deficient mutant of Chlamydomonas. / Minoda, Ayumi; Sonoike, Kintake; Okada, Katsuhiko; Sato, Norihiro; Tsuzuki, Mikio.

In: FEBS Letters, Vol. 553, No. 1-2, 09.10.2003, p. 109-112.

Research output: Contribution to journalArticle

Minoda, Ayumi ; Sonoike, Kintake ; Okada, Katsuhiko ; Sato, Norihiro ; Tsuzuki, Mikio. / Decrease in the efficiency of the electron donation to tyrosine Z of photosystem II in an SQDG-deficient mutant of Chlamydomonas. In: FEBS Letters. 2003 ; Vol. 553, No. 1-2. pp. 109-112.
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