Abstract
Photosystem (PS) II activity of a sulfoquinovosyl diacylglycerol (SQDG)-deficient mutant (hf-2) of Chlamydomonas was partially decreased compared with that of wild-type. The susceptibility to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was also modified in the mutant. Photometric measurements in the isolated thylakoid membranes of hf-2 revealed that the lowered activity in the mutant was derived from a decrease in the efficiency of the electron donation from water to tyrosine Z, not from the efficiency of the electron transport from QA to QB. This result was confirmed by the decay kinetics of chlorophyll fluorescence determined in vivo. We conclude that SQDG contributes to maintaining the conformation of PSII complexes, particularly that of D1 polypeptides, which are necessary for maximum activities in Chlamydomonas.
| Original language | English |
|---|---|
| Pages (from-to) | 109-112 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 553 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 2003 Oct 9 |
| Externally published | Yes |
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Keywords
- Chlamydomonas
- Glycolipid
- Photosystem II
- Sulfoquinovosyl diacylglycerol
- Thylakoid membrane
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Decrease in the efficiency of the electron donation to tyrosine Z of photosystem II in an SQDG-deficient mutant of Chlamydomonas. / Minoda, Ayumi; Sonoike, Kintake; Okada, Katsuhiko; Sato, Norihiro; Tsuzuki, Mikio.
In: FEBS Letters, Vol. 553, No. 1-2, 09.10.2003, p. 109-112.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Decrease in the efficiency of the electron donation to tyrosine Z of photosystem II in an SQDG-deficient mutant of Chlamydomonas
AU - Minoda, Ayumi
AU - Sonoike, Kintake
AU - Okada, Katsuhiko
AU - Sato, Norihiro
AU - Tsuzuki, Mikio
PY - 2003/10/9
Y1 - 2003/10/9
N2 - Photosystem (PS) II activity of a sulfoquinovosyl diacylglycerol (SQDG)-deficient mutant (hf-2) of Chlamydomonas was partially decreased compared with that of wild-type. The susceptibility to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was also modified in the mutant. Photometric measurements in the isolated thylakoid membranes of hf-2 revealed that the lowered activity in the mutant was derived from a decrease in the efficiency of the electron donation from water to tyrosine Z, not from the efficiency of the electron transport from QA to QB. This result was confirmed by the decay kinetics of chlorophyll fluorescence determined in vivo. We conclude that SQDG contributes to maintaining the conformation of PSII complexes, particularly that of D1 polypeptides, which are necessary for maximum activities in Chlamydomonas.
AB - Photosystem (PS) II activity of a sulfoquinovosyl diacylglycerol (SQDG)-deficient mutant (hf-2) of Chlamydomonas was partially decreased compared with that of wild-type. The susceptibility to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was also modified in the mutant. Photometric measurements in the isolated thylakoid membranes of hf-2 revealed that the lowered activity in the mutant was derived from a decrease in the efficiency of the electron donation from water to tyrosine Z, not from the efficiency of the electron transport from QA to QB. This result was confirmed by the decay kinetics of chlorophyll fluorescence determined in vivo. We conclude that SQDG contributes to maintaining the conformation of PSII complexes, particularly that of D1 polypeptides, which are necessary for maximum activities in Chlamydomonas.
KW - Chlamydomonas
KW - Glycolipid
KW - Photosystem II
KW - Sulfoquinovosyl diacylglycerol
KW - Thylakoid membrane
UR - http://www.scopus.com/inward/record.url?scp=0141794511&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0141794511&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(03)00981-5
DO - 10.1016/S0014-5793(03)00981-5
M3 - Article
C2 - 14550556
AN - SCOPUS:0141794511
VL - 553
SP - 109
EP - 112
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-2
ER -