Decrease of citric acid produced by Aspergillus Niger through disruption of the gene encoding a putative mitochondrial citrate-oxoglutarate shuttle protein

Kotaro Kirimura, Keiichi Kobayashi, Isato Yoshioka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The transporter that exports citric acid (CA) generated in mitochondria to the cytosol is an important component of the CA production machinery of Aspergillus Niger. In this report, we cloned and identified the gene cocA, encoding a 33.7-kDa putative mitochondrial citrate-oxoglutarate shuttle protein of the CA hyper-producer A. Niger WU-2223L. The amount of CA produced by a representative cocA disruptant (35 g/L) was significantly lower than that produced by strain WU-2223L (63 g/L) after culture for 12 days under CA production conditions, and the phenotype of the cocA disruptant differed in part from that of strain WU-2223L. A cocA disruptant complemented with cocA exhibited the same phenotypes as those of strain WU-2223L. This report is the first to show that cocA and its protein product clearly contribute to substantial CA production by A. Niger, and provides a significant insight into microbial organic acid production by fermentation.

Original languageEnglish
Pages (from-to)1538-1546
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume83
Issue number8
DOIs
Publication statusPublished - 2019 Jan 1

Keywords

  • Aspergillus Niger
  • Citrate transporter
  • Citrate-oxoglutarate shuttle protein
  • Citric acid production

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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