Decrease of serotonin and metabolite in the forebrain and facilitation of lordosis by dorsal raphe nucleus lesions in male rats

Masaki Kakeyama, Asami Umino, Toru Nishikawa, Korehito Yamanouchi

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    In castrated male rats, a radiofrequency lesion was made in the dorsal raphe nucleus (DRL) and lordosis behavior was observed following treatment with estrogen. After the behavioral test, brains were removed and the contents of 5-HT and 5-HIAA in the forebrain were measured by high pressure liquid chromatography (HPLC). In the results, only 2 of 16 control males without brain surgery showed lordosis, and the mean lordosis quotient (LQ) was extremely low when compared to that in control females. In contrast, all male rats with DRL displayed lordosis and the mean LQ was higher than that of control males without brain surgery but lower than that in control females (P<0.001). In the DRL males, 5-HT and 5-HIAA contents in the septum (SPT), the preoptic area (POA), the ventromedial hypothalamus (VMH) and the striatum (STM) were lower than those in control male and female groups (P<0.001). These results suggest that the dorsal raphe nucleus prevents male rats from showing lordosis by serotonergic influence in the forebrain. In addition, HPLC results showed that levels of the 5-HT in the SPT, the POA and the VMH in the female group were higher than those in the control male group (P<0.05). In female rats, the POA (P<0.01) and the VMH (P<0.05) contained larger 5-HT than those in the SPT and the STM, but there were no difference of 5-HT contents in the male rat.

    Original languageEnglish
    Pages (from-to)573-579
    Number of pages7
    JournalEndocrine Journal
    Volume49
    Issue number5
    DOIs
    Publication statusPublished - 2002 Oct

    Fingerprint

    Lordosis
    Prosencephalon
    Serotonin
    Preoptic Area
    Hypothalamus
    Hydroxyindoleacetic Acid
    Brain
    High Pressure Liquid Chromatography
    Dorsal Raphe Nucleus
    Estrogens
    Control Groups

    Keywords

    • Dorsal raphe nucleus
    • Forbrain
    • Lordosis
    • Male rat
    • Serotonin

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Decrease of serotonin and metabolite in the forebrain and facilitation of lordosis by dorsal raphe nucleus lesions in male rats. / Kakeyama, Masaki; Umino, Asami; Nishikawa, Toru; Yamanouchi, Korehito.

    In: Endocrine Journal, Vol. 49, No. 5, 10.2002, p. 573-579.

    Research output: Contribution to journalArticle

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