Abstract
Systemic lupus erythematosus (SLE) patients have a decreased number of peripheral blood T cells containing signal-joint T cell receptor excision circles (Sj TRECs), which are considered an indicator of thymic output. The objective of this study was to investigate the mechanism of the decrease in such T cells. Peripheral blood T cells from SLE patients were classified into CD4+ and CD8+ cells. Sj TREC levels were measured by real-time PCR. Telomerase activity was determined by the telomeric repeat amplification protocol assay. The numbers of Sj TREC containing CD4+ and CD8+ cells were lower in the peripheral blood of SLE patients than in the controls. A correlation was found between the numbers of Sj TREC-positive CD4+ and CD8+ cells. The level of TRECs is influenced by an increase in cell division. To examine this increase, telomerase activity as an indicator of cell division was measured simultaneously; however, there was no correlation between the Sj TREC level and telomerase activity. These results suggest that decreased thymic output occurs in SLE patients.
| Original language | English |
|---|---|
| Pages (from-to) | 296-300 |
| Number of pages | 5 |
| Journal | Modern Rheumatology |
| Volume | 17 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2007 Aug |
| Externally published | Yes |
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Keywords
- CD4
- CD8
- SLE
- T lymphocyte
- Tcell receptor excision circles (TRECs)
- Telomerase
ASJC Scopus subject areas
- Rheumatology
- Immunology
Cite this
Decreased numbers of signal-joint T cell receptor excision circle-containing CD4+ and CD8+ cells in systemic lupus erythematosus patients. / Kurosaka, Daitaro; Yasuda, Jun; Ikeshima-Kataoka, Hiroko; Ozawa, Yoshinori; Yoshida, Ken; Yasuda, Chiho; Kingetsu, Isamu; Saito, Saburo; Yamada, Akio.
In: Modern Rheumatology, Vol. 17, No. 4, 08.2007, p. 296-300.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Decreased numbers of signal-joint T cell receptor excision circle-containing CD4+ and CD8+ cells in systemic lupus erythematosus patients
AU - Kurosaka, Daitaro
AU - Yasuda, Jun
AU - Ikeshima-Kataoka, Hiroko
AU - Ozawa, Yoshinori
AU - Yoshida, Ken
AU - Yasuda, Chiho
AU - Kingetsu, Isamu
AU - Saito, Saburo
AU - Yamada, Akio
PY - 2007/8
Y1 - 2007/8
N2 - Systemic lupus erythematosus (SLE) patients have a decreased number of peripheral blood T cells containing signal-joint T cell receptor excision circles (Sj TRECs), which are considered an indicator of thymic output. The objective of this study was to investigate the mechanism of the decrease in such T cells. Peripheral blood T cells from SLE patients were classified into CD4+ and CD8+ cells. Sj TREC levels were measured by real-time PCR. Telomerase activity was determined by the telomeric repeat amplification protocol assay. The numbers of Sj TREC containing CD4+ and CD8+ cells were lower in the peripheral blood of SLE patients than in the controls. A correlation was found between the numbers of Sj TREC-positive CD4+ and CD8+ cells. The level of TRECs is influenced by an increase in cell division. To examine this increase, telomerase activity as an indicator of cell division was measured simultaneously; however, there was no correlation between the Sj TREC level and telomerase activity. These results suggest that decreased thymic output occurs in SLE patients.
AB - Systemic lupus erythematosus (SLE) patients have a decreased number of peripheral blood T cells containing signal-joint T cell receptor excision circles (Sj TRECs), which are considered an indicator of thymic output. The objective of this study was to investigate the mechanism of the decrease in such T cells. Peripheral blood T cells from SLE patients were classified into CD4+ and CD8+ cells. Sj TREC levels were measured by real-time PCR. Telomerase activity was determined by the telomeric repeat amplification protocol assay. The numbers of Sj TREC containing CD4+ and CD8+ cells were lower in the peripheral blood of SLE patients than in the controls. A correlation was found between the numbers of Sj TREC-positive CD4+ and CD8+ cells. The level of TRECs is influenced by an increase in cell division. To examine this increase, telomerase activity as an indicator of cell division was measured simultaneously; however, there was no correlation between the Sj TREC level and telomerase activity. These results suggest that decreased thymic output occurs in SLE patients.
KW - CD4
KW - CD8
KW - SLE
KW - T lymphocyte
KW - Tcell receptor excision circles (TRECs)
KW - Telomerase
UR - http://www.scopus.com/inward/record.url?scp=34547951878&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34547951878&partnerID=8YFLogxK
U2 - 10.1007/s10165-007-0583-x
DO - 10.1007/s10165-007-0583-x
M3 - Article
VL - 17
SP - 296
EP - 300
JO - Modern Rheumatology
JF - Modern Rheumatology
SN - 1439-7595
IS - 4
ER -