Decreased numbers of signal-joint T cell receptor excision circle-containing CD4+ and CD8+ cells in systemic lupus erythematosus patients

Daitaro Kurosaka, Jun Yasuda, Hiroko Ikeshima-Kataoka, Yoshinori Ozawa, Ken Yoshida, Chiho Yasuda, Isamu Kingetsu, Saburo Saito, Akio Yamada

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Systemic lupus erythematosus (SLE) patients have a decreased number of peripheral blood T cells containing signal-joint T cell receptor excision circles (Sj TRECs), which are considered an indicator of thymic output. The objective of this study was to investigate the mechanism of the decrease in such T cells. Peripheral blood T cells from SLE patients were classified into CD4+ and CD8+ cells. Sj TREC levels were measured by real-time PCR. Telomerase activity was determined by the telomeric repeat amplification protocol assay. The numbers of Sj TREC containing CD4+ and CD8+ cells were lower in the peripheral blood of SLE patients than in the controls. A correlation was found between the numbers of Sj TREC-positive CD4+ and CD8+ cells. The level of TRECs is influenced by an increase in cell division. To examine this increase, telomerase activity as an indicator of cell division was measured simultaneously; however, there was no correlation between the Sj TREC level and telomerase activity. These results suggest that decreased thymic output occurs in SLE patients.

Original languageEnglish
Pages (from-to)296-300
Number of pages5
JournalModern Rheumatology
Volume17
Issue number4
DOIs
Publication statusPublished - 2007 Aug
Externally publishedYes

Fingerprint

T-Cell Antigen Receptor
Systemic Lupus Erythematosus
Telomerase
Joints
T-Lymphocytes
Cell Division
Blood Cells
Real-Time Polymerase Chain Reaction

Keywords

  • CD4
  • CD8
  • SLE
  • T lymphocyte
  • Tcell receptor excision circles (TRECs)
  • Telomerase

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

Decreased numbers of signal-joint T cell receptor excision circle-containing CD4+ and CD8+ cells in systemic lupus erythematosus patients. / Kurosaka, Daitaro; Yasuda, Jun; Ikeshima-Kataoka, Hiroko; Ozawa, Yoshinori; Yoshida, Ken; Yasuda, Chiho; Kingetsu, Isamu; Saito, Saburo; Yamada, Akio.

In: Modern Rheumatology, Vol. 17, No. 4, 08.2007, p. 296-300.

Research output: Contribution to journalArticle

Kurosaka, D, Yasuda, J, Ikeshima-Kataoka, H, Ozawa, Y, Yoshida, K, Yasuda, C, Kingetsu, I, Saito, S & Yamada, A 2007, 'Decreased numbers of signal-joint T cell receptor excision circle-containing CD4+ and CD8+ cells in systemic lupus erythematosus patients', Modern Rheumatology, vol. 17, no. 4, pp. 296-300. https://doi.org/10.1007/s10165-007-0583-x
Kurosaka, Daitaro ; Yasuda, Jun ; Ikeshima-Kataoka, Hiroko ; Ozawa, Yoshinori ; Yoshida, Ken ; Yasuda, Chiho ; Kingetsu, Isamu ; Saito, Saburo ; Yamada, Akio. / Decreased numbers of signal-joint T cell receptor excision circle-containing CD4+ and CD8+ cells in systemic lupus erythematosus patients. In: Modern Rheumatology. 2007 ; Vol. 17, No. 4. pp. 296-300.
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