Decreased numbers of signal-joint T cell receptor excision circle-containing CD4+ and CD8+ cells in systemic lupus erythematosus patients

Daitaro Kurosaka, Jun Yasuda, Hiroko Ikeshima-Kataoka, Yoshinori Ozawa, Ken Yoshida, Chiho Yasuda, Isamu Kingetsu, Saburo Saito, Akio Yamada

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8 Citations (Scopus)

Abstract

Systemic lupus erythematosus (SLE) patients have a decreased number of peripheral blood T cells containing signal-joint T cell receptor excision circles (Sj TRECs), which are considered an indicator of thymic output. The objective of this study was to investigate the mechanism of the decrease in such T cells. Peripheral blood T cells from SLE patients were classified into CD4+ and CD8+ cells. Sj TREC levels were measured by real-time PCR. Telomerase activity was determined by the telomeric repeat amplification protocol assay. The numbers of Sj TREC containing CD4+ and CD8+ cells were lower in the peripheral blood of SLE patients than in the controls. A correlation was found between the numbers of Sj TREC-positive CD4+ and CD8+ cells. The level of TRECs is influenced by an increase in cell division. To examine this increase, telomerase activity as an indicator of cell division was measured simultaneously; however, there was no correlation between the Sj TREC level and telomerase activity. These results suggest that decreased thymic output occurs in SLE patients.

Original languageEnglish
Pages (from-to)296-300
Number of pages5
JournalModern Rheumatology
Volume17
Issue number4
DOIs
Publication statusPublished - 2007 Aug
Externally publishedYes

Keywords

  • CD4
  • CD8
  • SLE
  • T lymphocyte
  • Tcell receptor excision circles (TRECs)
  • Telomerase

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

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    Kurosaka, D., Yasuda, J., Ikeshima-Kataoka, H., Ozawa, Y., Yoshida, K., Yasuda, C., Kingetsu, I., Saito, S., & Yamada, A. (2007). Decreased numbers of signal-joint T cell receptor excision circle-containing CD4+ and CD8+ cells in systemic lupus erythematosus patients. Modern Rheumatology, 17(4), 296-300. https://doi.org/10.1007/s10165-007-0583-x