Decreased numbers of signal-joint T cell receptor excision circle-containing CD4+ and CD8+ cells in systemic lupus erythematosus patients

Daitaro Kurosaka; Jun Yasuda; Hiroko Ikeshima-Kataoka; Yoshinori Ozawa; Ken Yoshida; Chiho Yasuda; Isamu Kingetsu; Saburo Saito; Akio Yamada

doi:10.1007/s10165-007-0583-x

Decreased numbers of signal-joint T cell receptor excision circle-containing CD4+ and CD8+ cells in systemic lupus erythematosus patients

Daitaro Kurosaka, Jun Yasuda, Hiroko Ikeshima-Kataoka, Yoshinori Ozawa, Ken Yoshida, Chiho Yasuda, Isamu Kingetsu, Saburo Saito, Akio Yamada

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Systemic lupus erythematosus (SLE) patients have a decreased number of peripheral blood T cells containing signal-joint T cell receptor excision circles (Sj TRECs), which are considered an indicator of thymic output. The objective of this study was to investigate the mechanism of the decrease in such T cells. Peripheral blood T cells from SLE patients were classified into CD4+ and CD8+ cells. Sj TREC levels were measured by real-time PCR. Telomerase activity was determined by the telomeric repeat amplification protocol assay. The numbers of Sj TREC containing CD4+ and CD8+ cells were lower in the peripheral blood of SLE patients than in the controls. A correlation was found between the numbers of Sj TREC-positive CD4+ and CD8+ cells. The level of TRECs is influenced by an increase in cell division. To examine this increase, telomerase activity as an indicator of cell division was measured simultaneously; however, there was no correlation between the Sj TREC level and telomerase activity. These results suggest that decreased thymic output occurs in SLE patients.

Original language	English
Pages (from-to)	296-300
Number of pages	5
Journal	Modern Rheumatology
Volume	17
Issue number	4
DOIs	https://doi.org/10.1007/s10165-007-0583-x
Publication status	Published - 2007 Aug
Externally published	Yes

Keywords

CD4
CD8
SLE
T lymphocyte
Tcell receptor excision circles (TRECs)
Telomerase

ASJC Scopus subject areas

Rheumatology
Immunology

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Decreased numbers of signal-joint T cell receptor excision circle-containing CD4+ and CD8+ cells in systemic lupus erythematosus patients. / Kurosaka, Daitaro; Yasuda, Jun; Ikeshima-Kataoka, Hiroko; Ozawa, Yoshinori; Yoshida, Ken; Yasuda, Chiho; Kingetsu, Isamu; Saito, Saburo; Yamada, Akio.

In: Modern Rheumatology, Vol. 17, No. 4, 08.2007, p. 296-300.

Research output: Contribution to journal › Article › peer-review

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abstract = "Systemic lupus erythematosus (SLE) patients have a decreased number of peripheral blood T cells containing signal-joint T cell receptor excision circles (Sj TRECs), which are considered an indicator of thymic output. The objective of this study was to investigate the mechanism of the decrease in such T cells. Peripheral blood T cells from SLE patients were classified into CD4+ and CD8+ cells. Sj TREC levels were measured by real-time PCR. Telomerase activity was determined by the telomeric repeat amplification protocol assay. The numbers of Sj TREC containing CD4+ and CD8+ cells were lower in the peripheral blood of SLE patients than in the controls. A correlation was found between the numbers of Sj TREC-positive CD4+ and CD8+ cells. The level of TRECs is influenced by an increase in cell division. To examine this increase, telomerase activity as an indicator of cell division was measured simultaneously; however, there was no correlation between the Sj TREC level and telomerase activity. These results suggest that decreased thymic output occurs in SLE patients.",

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