Deformability and adhesive force of artificial platelets measured by atomic force microscopy

Toru Wada, Yosuke Okamura, Shinji Takeoka, Ryo Sudo, Yasuo Ikeda, Kazuo Tanishita

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Platelet glycoprotein GPIaIIa is an adhesive protein that recognizes collagen. We have investigated polymerized albumin particles conjugated with recombinant GPIaIIa (rGPIaIIa-poly Alb) for their platelet-like function. To evaluate the feasibility of these particles to achieve the hemostatic process, we measured the deformability (Young's modulus and spring constant) and the adhesive force of the particles using atomic force microscopy, which can measure the mechanical properties of individual cells. Our results showed that the Young's modulus of these particles was 2.3-fold larger than that of natural platelets and 12-fold larger than that of human red blood cells. The Young's modulus of the particles may have been determined by the properties of the polymerized albumin particle, although the glycoprotein of the platelet surface also contributed to the higher modulus. Our results also showed that the adhesive force of the rGPIaIIa-poly Alb with the collagen ligand was 52% of that of natural platelets. These two mechanical properties (deformability and adhesive force) of cells or particles, such as rGPIaIIa-poly Alb, are important specifications for the optimum design of platelet substitutes.

    Original languageEnglish
    Pages (from-to)35-40
    Number of pages6
    JournalJournal of Biorheology
    Volume23
    Issue number1
    DOIs
    Publication statusPublished - 2009

    Fingerprint

    Formability
    Platelets
    Atomic force microscopy
    Adhesives
    Platelet Membrane Glycoproteins
    Elastic moduli
    Albumins
    Glycoproteins
    Collagen
    Mechanical properties
    Hemostatics
    Blood
    Cells
    Ligands
    Specifications
    Proteins

    Keywords

    • Adhesive force
    • Atomic force microscopy
    • Deformability
    • Glycoprotein GPIaIIa
    • Platelet substitutes
    • Thrombocytopenia
    • Young's modulus

    ASJC Scopus subject areas

    • Mechanics of Materials
    • Materials Science(all)
    • Mechanical Engineering

    Cite this

    Deformability and adhesive force of artificial platelets measured by atomic force microscopy. / Wada, Toru; Okamura, Yosuke; Takeoka, Shinji; Sudo, Ryo; Ikeda, Yasuo; Tanishita, Kazuo.

    In: Journal of Biorheology, Vol. 23, No. 1, 2009, p. 35-40.

    Research output: Contribution to journalArticle

    Wada, Toru ; Okamura, Yosuke ; Takeoka, Shinji ; Sudo, Ryo ; Ikeda, Yasuo ; Tanishita, Kazuo. / Deformability and adhesive force of artificial platelets measured by atomic force microscopy. In: Journal of Biorheology. 2009 ; Vol. 23, No. 1. pp. 35-40.
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    AU - Ikeda, Yasuo

    AU - Tanishita, Kazuo

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