@inproceedings{ffaa4e3605514da8a0d68b312109742b,
title = "Deformability-based microfluidic microdroplet sorting as a screening method for single agarolytic bacterial cells",
abstract = "We have developed a deformability-based microfluidic microdroplet method for screening microbial cells secreting macromolecule-degrading enzymes. In this method, microbial cells are encapsulated as single cells in water-in-oil (W/O) microdroplets with macromolecule, whose shape becomes deformable as the macromolecule is progressively degraded into smaller molecules. Screening is achieved using a microfluidic device that passively sorts the deformed W/O microdroplets. Using this method, we successfully sorted agarose-containing microdroplets encapsulating single bacterial cells that hydrolyze agarose.",
keywords = "Agarose, Deformability, Microdroplet, Screening, Sorting",
author = "Mikihisa Muta and Kai Saito and Ryo Iizuka and Wataru Kawakubo and Yoon, {Dong Hyun} and Mei Ito and Yuji Hatada and Tetsushi Sekiguchi and Shuichi Shoji and Takashi Funatsu",
note = "Publisher Copyright: {\textcopyright} 2020 CBMS-0001; 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 ; Conference date: 04-10-2020 Through 09-10-2020",
year = "2020",
language = "English",
series = "MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "48--49",
booktitle = "MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
}