Deformability-based microfluidic microdroplet sorting as a screening method for single agarolytic bacterial cells

Mikihisa Muta, Kai Saito, Ryo Iizuka, Wataru Kawakubo, Dong Hyun Yoon, Mei Ito, Yuji Hatada, Tetsushi Sekiguchi, Shuichi Shoji, Takashi Funatsu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed a deformability-based microfluidic microdroplet method for screening microbial cells secreting macromolecule-degrading enzymes. In this method, microbial cells are encapsulated as single cells in water-in-oil (W/O) microdroplets with macromolecule, whose shape becomes deformable as the macromolecule is progressively degraded into smaller molecules. Screening is achieved using a microfluidic device that passively sorts the deformed W/O microdroplets. Using this method, we successfully sorted agarose-containing microdroplets encapsulating single bacterial cells that hydrolyze agarose.

Original languageEnglish
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages48-49
Number of pages2
ISBN (Electronic)9781733419017
Publication statusPublished - 2020
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: 2020 Oct 42020 Oct 9

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period20/10/420/10/9

Keywords

  • Agarose
  • Deformability
  • Microdroplet
  • Screening
  • Sorting

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering
  • Chemistry(all)
  • Control and Systems Engineering

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