Degeneration of dopaminergic neurons in the substantia nigra of zitter mutant rat and protection by chronic intake of Vitamin E

Shuichi Ueda, Shinichi Sakakibara, Kazuhiko Nakadate, Takahiro Noda, Motoo Shinoda, Jeffrey N. Joyce

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Dopaminergic cell death in the ventral and dorsal tiers of substantia nigra pars copmacta (SNc) and their prevention by anti-oxidant diet was immunohistochemically studied in the zitter mutant rats, which are characterized by abnormal metabolism of superoxide. Similar to previous reports, the number of SNc neurons in Nissl-stained section decreased with age. Tyrosine hydroxylase (TH) immunohistochemistry demonstrated that the dopaminergic neurons in the ventral tier of SNc degenerated early, whereas the dorsal tier gradually degenerated with age. Thus, the ventral tier dopaminergic neurons are affected first, but the dorsal tier neurons do become impact by the zi/zi mutation. Following 9-month period after weaning, zitter rats supplemented with 500 mg d,l-α-tocophenol (VE(+))/kg diet exhibited a significant increased of surviving TH-immunoreactive neurons in both the tiers of SNc as compared with the zi/zi rats with control and VE(-) diets. These results suggest that VE supplement may slow the dopaminergic cell loss in zitter mutant rat, and further support that degeneration of the dopaminergic neurons in this mutant rat is caused by oxidant stress. Thus, the zitter rat may represent a good model for studying the dopaminergic cell death by superoxide species.

Original languageEnglish
Pages (from-to)252-256
Number of pages5
JournalNeuroscience Letters
Volume380
Issue number3
DOIs
Publication statusPublished - 2005 Jun 3
Externally publishedYes

Fingerprint

Dopaminergic Neurons
Substantia Nigra
Vitamin E
Tyrosine 3-Monooxygenase
Diet
Neurons
Oxidants
Superoxides
Cell Death
Weaning
Immunohistochemistry
Mutation

Keywords

  • Dopamine
  • Immunohistochemistry
  • Neuroprotection
  • Oxidant stress
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Degeneration of dopaminergic neurons in the substantia nigra of zitter mutant rat and protection by chronic intake of Vitamin E. / Ueda, Shuichi; Sakakibara, Shinichi; Nakadate, Kazuhiko; Noda, Takahiro; Shinoda, Motoo; Joyce, Jeffrey N.

In: Neuroscience Letters, Vol. 380, No. 3, 03.06.2005, p. 252-256.

Research output: Contribution to journalArticle

Ueda, Shuichi ; Sakakibara, Shinichi ; Nakadate, Kazuhiko ; Noda, Takahiro ; Shinoda, Motoo ; Joyce, Jeffrey N. / Degeneration of dopaminergic neurons in the substantia nigra of zitter mutant rat and protection by chronic intake of Vitamin E. In: Neuroscience Letters. 2005 ; Vol. 380, No. 3. pp. 252-256.
@article{7f614054a647477985902e8d4e1ca77d,
title = "Degeneration of dopaminergic neurons in the substantia nigra of zitter mutant rat and protection by chronic intake of Vitamin E",
abstract = "Dopaminergic cell death in the ventral and dorsal tiers of substantia nigra pars copmacta (SNc) and their prevention by anti-oxidant diet was immunohistochemically studied in the zitter mutant rats, which are characterized by abnormal metabolism of superoxide. Similar to previous reports, the number of SNc neurons in Nissl-stained section decreased with age. Tyrosine hydroxylase (TH) immunohistochemistry demonstrated that the dopaminergic neurons in the ventral tier of SNc degenerated early, whereas the dorsal tier gradually degenerated with age. Thus, the ventral tier dopaminergic neurons are affected first, but the dorsal tier neurons do become impact by the zi/zi mutation. Following 9-month period after weaning, zitter rats supplemented with 500 mg d,l-α-tocophenol (VE(+))/kg diet exhibited a significant increased of surviving TH-immunoreactive neurons in both the tiers of SNc as compared with the zi/zi rats with control and VE(-) diets. These results suggest that VE supplement may slow the dopaminergic cell loss in zitter mutant rat, and further support that degeneration of the dopaminergic neurons in this mutant rat is caused by oxidant stress. Thus, the zitter rat may represent a good model for studying the dopaminergic cell death by superoxide species.",
keywords = "Dopamine, Immunohistochemistry, Neuroprotection, Oxidant stress, Tyrosine hydroxylase",
author = "Shuichi Ueda and Shinichi Sakakibara and Kazuhiko Nakadate and Takahiro Noda and Motoo Shinoda and Joyce, {Jeffrey N.}",
year = "2005",
month = "6",
day = "3",
doi = "10.1016/j.neulet.2005.01.053",
language = "English",
volume = "380",
pages = "252--256",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

TY - JOUR

T1 - Degeneration of dopaminergic neurons in the substantia nigra of zitter mutant rat and protection by chronic intake of Vitamin E

AU - Ueda, Shuichi

AU - Sakakibara, Shinichi

AU - Nakadate, Kazuhiko

AU - Noda, Takahiro

AU - Shinoda, Motoo

AU - Joyce, Jeffrey N.

PY - 2005/6/3

Y1 - 2005/6/3

N2 - Dopaminergic cell death in the ventral and dorsal tiers of substantia nigra pars copmacta (SNc) and their prevention by anti-oxidant diet was immunohistochemically studied in the zitter mutant rats, which are characterized by abnormal metabolism of superoxide. Similar to previous reports, the number of SNc neurons in Nissl-stained section decreased with age. Tyrosine hydroxylase (TH) immunohistochemistry demonstrated that the dopaminergic neurons in the ventral tier of SNc degenerated early, whereas the dorsal tier gradually degenerated with age. Thus, the ventral tier dopaminergic neurons are affected first, but the dorsal tier neurons do become impact by the zi/zi mutation. Following 9-month period after weaning, zitter rats supplemented with 500 mg d,l-α-tocophenol (VE(+))/kg diet exhibited a significant increased of surviving TH-immunoreactive neurons in both the tiers of SNc as compared with the zi/zi rats with control and VE(-) diets. These results suggest that VE supplement may slow the dopaminergic cell loss in zitter mutant rat, and further support that degeneration of the dopaminergic neurons in this mutant rat is caused by oxidant stress. Thus, the zitter rat may represent a good model for studying the dopaminergic cell death by superoxide species.

AB - Dopaminergic cell death in the ventral and dorsal tiers of substantia nigra pars copmacta (SNc) and their prevention by anti-oxidant diet was immunohistochemically studied in the zitter mutant rats, which are characterized by abnormal metabolism of superoxide. Similar to previous reports, the number of SNc neurons in Nissl-stained section decreased with age. Tyrosine hydroxylase (TH) immunohistochemistry demonstrated that the dopaminergic neurons in the ventral tier of SNc degenerated early, whereas the dorsal tier gradually degenerated with age. Thus, the ventral tier dopaminergic neurons are affected first, but the dorsal tier neurons do become impact by the zi/zi mutation. Following 9-month period after weaning, zitter rats supplemented with 500 mg d,l-α-tocophenol (VE(+))/kg diet exhibited a significant increased of surviving TH-immunoreactive neurons in both the tiers of SNc as compared with the zi/zi rats with control and VE(-) diets. These results suggest that VE supplement may slow the dopaminergic cell loss in zitter mutant rat, and further support that degeneration of the dopaminergic neurons in this mutant rat is caused by oxidant stress. Thus, the zitter rat may represent a good model for studying the dopaminergic cell death by superoxide species.

KW - Dopamine

KW - Immunohistochemistry

KW - Neuroprotection

KW - Oxidant stress

KW - Tyrosine hydroxylase

UR - http://www.scopus.com/inward/record.url?scp=18144378453&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18144378453&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2005.01.053

DO - 10.1016/j.neulet.2005.01.053

M3 - Article

C2 - 15862896

AN - SCOPUS:18144378453

VL - 380

SP - 252

EP - 256

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 3

ER -