Abstract
Dimethyl sulfoxide (DMSO)-degrading ability of Hyphomicrobium denitrificans WU-K217 immobilized by entrapment with calcium-alginate gel was examined. The immobilized cells with the cell density of 0.46 mg-dry weight ml-1 degraded 0.64 mM (50 mgl-1) DMSO within 180 min at a temperature range from 20 to 35 °C, similarly to the freely-suspended cells. At 40 °C, the immobilized cells completely degraded 0.64 mM DMSO within 240 min, although the freely-suspended cells could not degrade it under the same condition. The half-life of DMSO-degradation activity for the immobilized cells stored at 30 °C was 96 h and it was extended to more than 240 h by the storage at 4 °C. However, in the case of the freely-suspended cells, the half-life was shorter than 48 h even by the storage at 4 °C. These results indicated that the immobilized WU-K217 cells had high thermostability and storage stability compared to those of the freely-suspended cells. Considering these characteristics, we performed the repeated DMSO degradation by reuse of the immobilized cells. As a result, even at the tenth reaction, the immobilized cells maintained 90% of DMSO-degradation rate of that at the first reaction. These results suggested that the immobilized cells of WU-K217 might be applicable to a wastewater-treatment system for the removal of DMSO.
| Original language | English |
|---|---|
| Pages (from-to) | 199-204 |
| Number of pages | 6 |
| Journal | Biochemical Engineering Journal |
| Volume | 15 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2003 Sep |
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Keywords
- Biodegradation
- Dimethyl sulfoxide
- Hyphomicrobium denitrificans
- Immobilization
- Wastewater
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Chemical Engineering(all)
Cite this
Degradation of dimethyl sulfoxide by the immobilized cells of Hyphomicrobium denitrificans WU-K217. / Murakami-Nitta, Takako; Kirimura, Kotaro; Kino, Kuniki.
In: Biochemical Engineering Journal, Vol. 15, No. 3, 09.2003, p. 199-204.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Degradation of dimethyl sulfoxide by the immobilized cells of Hyphomicrobium denitrificans WU-K217
AU - Murakami-Nitta, Takako
AU - Kirimura, Kotaro
AU - Kino, Kuniki
PY - 2003/9
Y1 - 2003/9
N2 - Dimethyl sulfoxide (DMSO)-degrading ability of Hyphomicrobium denitrificans WU-K217 immobilized by entrapment with calcium-alginate gel was examined. The immobilized cells with the cell density of 0.46 mg-dry weight ml-1 degraded 0.64 mM (50 mgl-1) DMSO within 180 min at a temperature range from 20 to 35 °C, similarly to the freely-suspended cells. At 40 °C, the immobilized cells completely degraded 0.64 mM DMSO within 240 min, although the freely-suspended cells could not degrade it under the same condition. The half-life of DMSO-degradation activity for the immobilized cells stored at 30 °C was 96 h and it was extended to more than 240 h by the storage at 4 °C. However, in the case of the freely-suspended cells, the half-life was shorter than 48 h even by the storage at 4 °C. These results indicated that the immobilized WU-K217 cells had high thermostability and storage stability compared to those of the freely-suspended cells. Considering these characteristics, we performed the repeated DMSO degradation by reuse of the immobilized cells. As a result, even at the tenth reaction, the immobilized cells maintained 90% of DMSO-degradation rate of that at the first reaction. These results suggested that the immobilized cells of WU-K217 might be applicable to a wastewater-treatment system for the removal of DMSO.
AB - Dimethyl sulfoxide (DMSO)-degrading ability of Hyphomicrobium denitrificans WU-K217 immobilized by entrapment with calcium-alginate gel was examined. The immobilized cells with the cell density of 0.46 mg-dry weight ml-1 degraded 0.64 mM (50 mgl-1) DMSO within 180 min at a temperature range from 20 to 35 °C, similarly to the freely-suspended cells. At 40 °C, the immobilized cells completely degraded 0.64 mM DMSO within 240 min, although the freely-suspended cells could not degrade it under the same condition. The half-life of DMSO-degradation activity for the immobilized cells stored at 30 °C was 96 h and it was extended to more than 240 h by the storage at 4 °C. However, in the case of the freely-suspended cells, the half-life was shorter than 48 h even by the storage at 4 °C. These results indicated that the immobilized WU-K217 cells had high thermostability and storage stability compared to those of the freely-suspended cells. Considering these characteristics, we performed the repeated DMSO degradation by reuse of the immobilized cells. As a result, even at the tenth reaction, the immobilized cells maintained 90% of DMSO-degradation rate of that at the first reaction. These results suggested that the immobilized cells of WU-K217 might be applicable to a wastewater-treatment system for the removal of DMSO.
KW - Biodegradation
KW - Dimethyl sulfoxide
KW - Hyphomicrobium denitrificans
KW - Immobilization
KW - Wastewater
UR - http://www.scopus.com/inward/record.url?scp=0038267640&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038267640&partnerID=8YFLogxK
U2 - 10.1016/S1369-703X(03)00004-4
DO - 10.1016/S1369-703X(03)00004-4
M3 - Article
AN - SCOPUS:0038267640
VL - 15
SP - 199
EP - 204
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
SN - 1369-703X
IS - 3
ER -