Degradation of dimethyl sulfoxide by the immobilized cells of Hyphomicrobium denitrificans WU-K217

Takako Murakami-Nitta, Kotaro Kirimura, Kuniki Kino

    Research output: Contribution to journalArticle

    21 Citations (Scopus)

    Abstract

    Dimethyl sulfoxide (DMSO)-degrading ability of Hyphomicrobium denitrificans WU-K217 immobilized by entrapment with calcium-alginate gel was examined. The immobilized cells with the cell density of 0.46 mg-dry weight ml-1 degraded 0.64 mM (50 mgl-1) DMSO within 180 min at a temperature range from 20 to 35 °C, similarly to the freely-suspended cells. At 40 °C, the immobilized cells completely degraded 0.64 mM DMSO within 240 min, although the freely-suspended cells could not degrade it under the same condition. The half-life of DMSO-degradation activity for the immobilized cells stored at 30 °C was 96 h and it was extended to more than 240 h by the storage at 4 °C. However, in the case of the freely-suspended cells, the half-life was shorter than 48 h even by the storage at 4 °C. These results indicated that the immobilized WU-K217 cells had high thermostability and storage stability compared to those of the freely-suspended cells. Considering these characteristics, we performed the repeated DMSO degradation by reuse of the immobilized cells. As a result, even at the tenth reaction, the immobilized cells maintained 90% of DMSO-degradation rate of that at the first reaction. These results suggested that the immobilized cells of WU-K217 might be applicable to a wastewater-treatment system for the removal of DMSO.

    Original languageEnglish
    Pages (from-to)199-204
    Number of pages6
    JournalBiochemical Engineering Journal
    Volume15
    Issue number3
    DOIs
    Publication statusPublished - 2003 Sep

    Fingerprint

    Hyphomicrobium
    Immobilized Cells
    Dimethyl sulfoxide
    Dimethyl Sulfoxide
    Cells
    Degradation
    Half-Life
    Alginate
    Waste Water
    Wastewater treatment
    Calcium
    Gels
    Cell Count
    Weights and Measures
    Temperature

    Keywords

    • Biodegradation
    • Dimethyl sulfoxide
    • Hyphomicrobium denitrificans
    • Immobilization
    • Wastewater

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Chemical Engineering(all)

    Cite this

    Degradation of dimethyl sulfoxide by the immobilized cells of Hyphomicrobium denitrificans WU-K217. / Murakami-Nitta, Takako; Kirimura, Kotaro; Kino, Kuniki.

    In: Biochemical Engineering Journal, Vol. 15, No. 3, 09.2003, p. 199-204.

    Research output: Contribution to journalArticle

    @article{a200ad655c284c56a4968975ab938271,
    title = "Degradation of dimethyl sulfoxide by the immobilized cells of Hyphomicrobium denitrificans WU-K217",
    abstract = "Dimethyl sulfoxide (DMSO)-degrading ability of Hyphomicrobium denitrificans WU-K217 immobilized by entrapment with calcium-alginate gel was examined. The immobilized cells with the cell density of 0.46 mg-dry weight ml-1 degraded 0.64 mM (50 mgl-1) DMSO within 180 min at a temperature range from 20 to 35 °C, similarly to the freely-suspended cells. At 40 °C, the immobilized cells completely degraded 0.64 mM DMSO within 240 min, although the freely-suspended cells could not degrade it under the same condition. The half-life of DMSO-degradation activity for the immobilized cells stored at 30 °C was 96 h and it was extended to more than 240 h by the storage at 4 °C. However, in the case of the freely-suspended cells, the half-life was shorter than 48 h even by the storage at 4 °C. These results indicated that the immobilized WU-K217 cells had high thermostability and storage stability compared to those of the freely-suspended cells. Considering these characteristics, we performed the repeated DMSO degradation by reuse of the immobilized cells. As a result, even at the tenth reaction, the immobilized cells maintained 90{\%} of DMSO-degradation rate of that at the first reaction. These results suggested that the immobilized cells of WU-K217 might be applicable to a wastewater-treatment system for the removal of DMSO.",
    keywords = "Biodegradation, Dimethyl sulfoxide, Hyphomicrobium denitrificans, Immobilization, Wastewater",
    author = "Takako Murakami-Nitta and Kotaro Kirimura and Kuniki Kino",
    year = "2003",
    month = "9",
    doi = "10.1016/S1369-703X(03)00004-4",
    language = "English",
    volume = "15",
    pages = "199--204",
    journal = "Biochemical Engineering Journal",
    issn = "1369-703X",
    publisher = "Elsevier",
    number = "3",

    }

    TY - JOUR

    T1 - Degradation of dimethyl sulfoxide by the immobilized cells of Hyphomicrobium denitrificans WU-K217

    AU - Murakami-Nitta, Takako

    AU - Kirimura, Kotaro

    AU - Kino, Kuniki

    PY - 2003/9

    Y1 - 2003/9

    N2 - Dimethyl sulfoxide (DMSO)-degrading ability of Hyphomicrobium denitrificans WU-K217 immobilized by entrapment with calcium-alginate gel was examined. The immobilized cells with the cell density of 0.46 mg-dry weight ml-1 degraded 0.64 mM (50 mgl-1) DMSO within 180 min at a temperature range from 20 to 35 °C, similarly to the freely-suspended cells. At 40 °C, the immobilized cells completely degraded 0.64 mM DMSO within 240 min, although the freely-suspended cells could not degrade it under the same condition. The half-life of DMSO-degradation activity for the immobilized cells stored at 30 °C was 96 h and it was extended to more than 240 h by the storage at 4 °C. However, in the case of the freely-suspended cells, the half-life was shorter than 48 h even by the storage at 4 °C. These results indicated that the immobilized WU-K217 cells had high thermostability and storage stability compared to those of the freely-suspended cells. Considering these characteristics, we performed the repeated DMSO degradation by reuse of the immobilized cells. As a result, even at the tenth reaction, the immobilized cells maintained 90% of DMSO-degradation rate of that at the first reaction. These results suggested that the immobilized cells of WU-K217 might be applicable to a wastewater-treatment system for the removal of DMSO.

    AB - Dimethyl sulfoxide (DMSO)-degrading ability of Hyphomicrobium denitrificans WU-K217 immobilized by entrapment with calcium-alginate gel was examined. The immobilized cells with the cell density of 0.46 mg-dry weight ml-1 degraded 0.64 mM (50 mgl-1) DMSO within 180 min at a temperature range from 20 to 35 °C, similarly to the freely-suspended cells. At 40 °C, the immobilized cells completely degraded 0.64 mM DMSO within 240 min, although the freely-suspended cells could not degrade it under the same condition. The half-life of DMSO-degradation activity for the immobilized cells stored at 30 °C was 96 h and it was extended to more than 240 h by the storage at 4 °C. However, in the case of the freely-suspended cells, the half-life was shorter than 48 h even by the storage at 4 °C. These results indicated that the immobilized WU-K217 cells had high thermostability and storage stability compared to those of the freely-suspended cells. Considering these characteristics, we performed the repeated DMSO degradation by reuse of the immobilized cells. As a result, even at the tenth reaction, the immobilized cells maintained 90% of DMSO-degradation rate of that at the first reaction. These results suggested that the immobilized cells of WU-K217 might be applicable to a wastewater-treatment system for the removal of DMSO.

    KW - Biodegradation

    KW - Dimethyl sulfoxide

    KW - Hyphomicrobium denitrificans

    KW - Immobilization

    KW - Wastewater

    UR - http://www.scopus.com/inward/record.url?scp=0038267640&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0038267640&partnerID=8YFLogxK

    U2 - 10.1016/S1369-703X(03)00004-4

    DO - 10.1016/S1369-703X(03)00004-4

    M3 - Article

    AN - SCOPUS:0038267640

    VL - 15

    SP - 199

    EP - 204

    JO - Biochemical Engineering Journal

    JF - Biochemical Engineering Journal

    SN - 1369-703X

    IS - 3

    ER -