Degradation of psaB gene product, the reaction center subunit of photosystem I, is caused during photoinhibition of photosystem I

Possible involvement of active oxygen species

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94 Citations (Scopus)

Abstract

Specific degradation of psaB gene product, one of the two large subunits of photosystem I (PS I) reaction-center, was observed during photoinhibition of PS I. In the in vitro photoinhibition using spinach thylakoid membranes, the degradation of psaB gene product gave rise to fragments of 51 kDa and 45 kDa. n-Propyl gallate, a scavenger of active oxygen species, suppressed the generation of these fragments. Addition of methyl viologen, which protects PS I from photoinhibition but increases the production of superoxide, suppressed the generation of 51 kDa fragment but increased the generation of 45 kDa fragment. It was concluded that interaction of active oxygen species with reduced electron acceptor in PS I results in inactivation of PS I and in generation of 51 kDa fragment. On the other hand, 45 kDa fragment was generated solely by the presence of active oxygen species independent of the inhibition of PS I activity.

Original languageEnglish
Pages (from-to)157-164
Number of pages8
JournalPlant Science
Volume115
Issue number2
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Photosystem I Protein Complex
photosystem I
photoinhibition
Reactive Oxygen Species
Genes
Degradation
degradation
genes
Propyl Gallate
propyl gallate
Thylakoids
Paraquat
Spinacia oleracea
paraquat
spinach
thylakoids
Superoxides
superoxide anion
inactivation
electrons

Keywords

  • Active oxygen
  • Chilling stress
  • Photoinhibition
  • Photosynthesis
  • Photosystem I
  • Subunit degradation

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

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title = "Degradation of psaB gene product, the reaction center subunit of photosystem I, is caused during photoinhibition of photosystem I: Possible involvement of active oxygen species",
abstract = "Specific degradation of psaB gene product, one of the two large subunits of photosystem I (PS I) reaction-center, was observed during photoinhibition of PS I. In the in vitro photoinhibition using spinach thylakoid membranes, the degradation of psaB gene product gave rise to fragments of 51 kDa and 45 kDa. n-Propyl gallate, a scavenger of active oxygen species, suppressed the generation of these fragments. Addition of methyl viologen, which protects PS I from photoinhibition but increases the production of superoxide, suppressed the generation of 51 kDa fragment but increased the generation of 45 kDa fragment. It was concluded that interaction of active oxygen species with reduced electron acceptor in PS I results in inactivation of PS I and in generation of 51 kDa fragment. On the other hand, 45 kDa fragment was generated solely by the presence of active oxygen species independent of the inhibition of PS I activity.",
keywords = "Active oxygen, Chilling stress, Photoinhibition, Photosynthesis, Photosystem I, Subunit degradation",
author = "Kintake Sonoike",
year = "1996",
language = "English",
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pages = "157--164",
journal = "Plant Science",
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T1 - Degradation of psaB gene product, the reaction center subunit of photosystem I, is caused during photoinhibition of photosystem I

T2 - Possible involvement of active oxygen species

AU - Sonoike, Kintake

PY - 1996

Y1 - 1996

N2 - Specific degradation of psaB gene product, one of the two large subunits of photosystem I (PS I) reaction-center, was observed during photoinhibition of PS I. In the in vitro photoinhibition using spinach thylakoid membranes, the degradation of psaB gene product gave rise to fragments of 51 kDa and 45 kDa. n-Propyl gallate, a scavenger of active oxygen species, suppressed the generation of these fragments. Addition of methyl viologen, which protects PS I from photoinhibition but increases the production of superoxide, suppressed the generation of 51 kDa fragment but increased the generation of 45 kDa fragment. It was concluded that interaction of active oxygen species with reduced electron acceptor in PS I results in inactivation of PS I and in generation of 51 kDa fragment. On the other hand, 45 kDa fragment was generated solely by the presence of active oxygen species independent of the inhibition of PS I activity.

AB - Specific degradation of psaB gene product, one of the two large subunits of photosystem I (PS I) reaction-center, was observed during photoinhibition of PS I. In the in vitro photoinhibition using spinach thylakoid membranes, the degradation of psaB gene product gave rise to fragments of 51 kDa and 45 kDa. n-Propyl gallate, a scavenger of active oxygen species, suppressed the generation of these fragments. Addition of methyl viologen, which protects PS I from photoinhibition but increases the production of superoxide, suppressed the generation of 51 kDa fragment but increased the generation of 45 kDa fragment. It was concluded that interaction of active oxygen species with reduced electron acceptor in PS I results in inactivation of PS I and in generation of 51 kDa fragment. On the other hand, 45 kDa fragment was generated solely by the presence of active oxygen species independent of the inhibition of PS I activity.

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KW - Chilling stress

KW - Photoinhibition

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