Abstract
Specific degradation of psaB gene product, one of the two large subunits of photosystem I (PS I) reaction-center, was observed during photoinhibition of PS I. In the in vitro photoinhibition using spinach thylakoid membranes, the degradation of psaB gene product gave rise to fragments of 51 kDa and 45 kDa. n-Propyl gallate, a scavenger of active oxygen species, suppressed the generation of these fragments. Addition of methyl viologen, which protects PS I from photoinhibition but increases the production of superoxide, suppressed the generation of 51 kDa fragment but increased the generation of 45 kDa fragment. It was concluded that interaction of active oxygen species with reduced electron acceptor in PS I results in inactivation of PS I and in generation of 51 kDa fragment. On the other hand, 45 kDa fragment was generated solely by the presence of active oxygen species independent of the inhibition of PS I activity.
| Original language | English |
|---|---|
| Pages (from-to) | 157-164 |
| Number of pages | 8 |
| Journal | Plant Science |
| Volume | 115 |
| Issue number | 2 |
| Publication status | Published - 1996 |
| Externally published | Yes |
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Keywords
- Active oxygen
- Chilling stress
- Photoinhibition
- Photosynthesis
- Photosystem I
- Subunit degradation
ASJC Scopus subject areas
- Plant Science
- Biochemistry
- Biotechnology
Cite this
Degradation of psaB gene product, the reaction center subunit of photosystem I, is caused during photoinhibition of photosystem I : Possible involvement of active oxygen species. / Sonoike, Kintake.
In: Plant Science, Vol. 115, No. 2, 1996, p. 157-164.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Degradation of psaB gene product, the reaction center subunit of photosystem I, is caused during photoinhibition of photosystem I
T2 - Possible involvement of active oxygen species
AU - Sonoike, Kintake
PY - 1996
Y1 - 1996
N2 - Specific degradation of psaB gene product, one of the two large subunits of photosystem I (PS I) reaction-center, was observed during photoinhibition of PS I. In the in vitro photoinhibition using spinach thylakoid membranes, the degradation of psaB gene product gave rise to fragments of 51 kDa and 45 kDa. n-Propyl gallate, a scavenger of active oxygen species, suppressed the generation of these fragments. Addition of methyl viologen, which protects PS I from photoinhibition but increases the production of superoxide, suppressed the generation of 51 kDa fragment but increased the generation of 45 kDa fragment. It was concluded that interaction of active oxygen species with reduced electron acceptor in PS I results in inactivation of PS I and in generation of 51 kDa fragment. On the other hand, 45 kDa fragment was generated solely by the presence of active oxygen species independent of the inhibition of PS I activity.
AB - Specific degradation of psaB gene product, one of the two large subunits of photosystem I (PS I) reaction-center, was observed during photoinhibition of PS I. In the in vitro photoinhibition using spinach thylakoid membranes, the degradation of psaB gene product gave rise to fragments of 51 kDa and 45 kDa. n-Propyl gallate, a scavenger of active oxygen species, suppressed the generation of these fragments. Addition of methyl viologen, which protects PS I from photoinhibition but increases the production of superoxide, suppressed the generation of 51 kDa fragment but increased the generation of 45 kDa fragment. It was concluded that interaction of active oxygen species with reduced electron acceptor in PS I results in inactivation of PS I and in generation of 51 kDa fragment. On the other hand, 45 kDa fragment was generated solely by the presence of active oxygen species independent of the inhibition of PS I activity.
KW - Active oxygen
KW - Chilling stress
KW - Photoinhibition
KW - Photosynthesis
KW - Photosystem I
KW - Subunit degradation
UR - http://www.scopus.com/inward/record.url?scp=0000628304&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0000628304&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0000628304
VL - 115
SP - 157
EP - 164
JO - Plant Science
JF - Plant Science
SN - 0168-9452
IS - 2
ER -