Delayed appearance of G-protein coupled signal transduction system in developing cerebellar Purkinje cell dendrites

Hiroshi Takagi, Hideyuki Takimizu, Tohru Yoshioka, Nobuyuki Suzuki, Etsuro Ito, Yoshihisa Kudo

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    To investigate the relation between the function of Ca2+-activated K+ channels and phosphoinositide turnover, we have examined the physiological and pharmacological characteristics of ionotropic and metabotropic quisqualate (QA) receptors in rat cerebellar Purkinje cells during development using the slice-patch method combined with Ca2+ imaging. The typical response to QA obtained from a rat on postnatal day (PND) 21 consisted of three components: (1) a fast inactivating inward current, (2) a slow inward current, and (3) a slow outward current. The slow inward current was abolished in Ca2+-free medium, while the fast inactivating inward current and the slow outward current remained unaffected. The slow outward current which appeared to be activated via a metabotropic receptor was not observed in the Purkinje cell of PND 7 rat, in which dendrites were poorly developed but its amplitude increased linearly with PND. QA caused significant increases in [Ca2+]i in the fully developed dendritic region of the Purkinje cells even in Ca2+-free medium, suggesting a dendritic localization of the metabotropic receptors.

    Original languageEnglish
    Pages (from-to)206-212
    Number of pages7
    JournalNeuroscience Research
    Volume15
    Issue number3
    DOIs
    Publication statusPublished - 1992

    Fingerprint

    Purkinje Cells
    Dendrites
    GTP-Binding Proteins
    Quisqualic Acid
    Signal Transduction
    Calcium-Activated Potassium Channels
    Phosphatidylinositols
    Pharmacology

    Keywords

    • Ca-activated K current
    • Ca-imaging
    • Development
    • Excitatory amino acid
    • Phosphoinositide turnover
    • Purkinje cell
    • Slice-patch clamp

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Delayed appearance of G-protein coupled signal transduction system in developing cerebellar Purkinje cell dendrites. / Takagi, Hiroshi; Takimizu, Hideyuki; Yoshioka, Tohru; Suzuki, Nobuyuki; Ito, Etsuro; Kudo, Yoshihisa.

    In: Neuroscience Research, Vol. 15, No. 3, 1992, p. 206-212.

    Research output: Contribution to journalArticle

    Takagi, Hiroshi ; Takimizu, Hideyuki ; Yoshioka, Tohru ; Suzuki, Nobuyuki ; Ito, Etsuro ; Kudo, Yoshihisa. / Delayed appearance of G-protein coupled signal transduction system in developing cerebellar Purkinje cell dendrites. In: Neuroscience Research. 1992 ; Vol. 15, No. 3. pp. 206-212.
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