Deletion of the protein kinase A/protein kinase G target SMTNL1 promotes an exercise-adapted phenotype in vascular smooth muscle

Anne A. Wooldridge, Christopher N. Fortner, Beata Lontay, Takayuki Akimoto, Ronald L. Neppl, Carie Facemire, Michael B. Datto, Ashley Kwon, Everett McCook, Ping Li, Shiliang Wang, Randy J. Thresher, Sara E. Miller, Jean Claude Perriard, Timothy P. Gavin, Robert C. Hickner, Thomas M. Coffman, Avril V. Somlyo, Zhen Yan, Timothy A J Haystead

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In vivo protein kinases A and G (PKA and PKG) coordinately phosphorylate a broad range of substrates to mediate their various physiological effects. The functions of many of these substrates have yet to be defined genetically. Herein we show a role for smoothelin-like protein 1 (SMTNL1), a novel in vivo target of PKG/PKA, in mediating vascular adaptations to exercise. Aortas from smtnl1-/- mice exhibited strikingly enhanced vasorelaxation before exercise, similar in extent to that achieved after endurance training of wild-type littermates. Additionally, contractile responses to α-adrenergic agonists were greatly attenuated. Immunological studies showed SMTNL1 is expressed in smooth muscle and type 2a striated muscle fibers. Consistent with a role in adaptations to exercise, smtnl1-/- mice also exhibited increased type 2a fibers before training and better performance after forced endurance training compared smtnl1+/+ mice. Furthermore, exercise was found to reduce expression of SMTNL1, particularly in female mice. In both muscle types, SMTNL1 is phosphorylated at Ser-301 in response to adrenergic signals. In vitro SMTNL1 suppresses myosin phosphatase activity through a substrate-directed effect, which is relieved by Ser-301 phosphorylation. Our findings suggest roles for SMTNL1 in cGMP/cAMP-mediated adaptations to exercise through mechanisms involving direct modulation of contractile activity.

Original languageEnglish
Pages (from-to)11850-11859
Number of pages10
JournalJournal of Biological Chemistry
Volume283
Issue number17
DOIs
Publication statusPublished - 2008 Apr 25
Externally publishedYes

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Cyclic GMP-Dependent Protein Kinases
Cyclic AMP-Dependent Protein Kinases
Vascular Smooth Muscle
Muscle
Phenotype
Proteins
Durability
Substrates
Myosin-Light-Chain Phosphatase
Adrenergic Agonists
Phosphorylation
Striated Muscle
Fibers
Vasodilation
Adrenergic Agents
Smooth Muscle
Blood Vessels
Aorta
Modulation
Muscles

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Deletion of the protein kinase A/protein kinase G target SMTNL1 promotes an exercise-adapted phenotype in vascular smooth muscle. / Wooldridge, Anne A.; Fortner, Christopher N.; Lontay, Beata; Akimoto, Takayuki; Neppl, Ronald L.; Facemire, Carie; Datto, Michael B.; Kwon, Ashley; McCook, Everett; Li, Ping; Wang, Shiliang; Thresher, Randy J.; Miller, Sara E.; Perriard, Jean Claude; Gavin, Timothy P.; Hickner, Robert C.; Coffman, Thomas M.; Somlyo, Avril V.; Yan, Zhen; Haystead, Timothy A J.

In: Journal of Biological Chemistry, Vol. 283, No. 17, 25.04.2008, p. 11850-11859.

Research output: Contribution to journalArticle

Wooldridge, AA, Fortner, CN, Lontay, B, Akimoto, T, Neppl, RL, Facemire, C, Datto, MB, Kwon, A, McCook, E, Li, P, Wang, S, Thresher, RJ, Miller, SE, Perriard, JC, Gavin, TP, Hickner, RC, Coffman, TM, Somlyo, AV, Yan, Z & Haystead, TAJ 2008, 'Deletion of the protein kinase A/protein kinase G target SMTNL1 promotes an exercise-adapted phenotype in vascular smooth muscle', Journal of Biological Chemistry, vol. 283, no. 17, pp. 11850-11859. https://doi.org/10.1074/jbc.M708628200
Wooldridge, Anne A. ; Fortner, Christopher N. ; Lontay, Beata ; Akimoto, Takayuki ; Neppl, Ronald L. ; Facemire, Carie ; Datto, Michael B. ; Kwon, Ashley ; McCook, Everett ; Li, Ping ; Wang, Shiliang ; Thresher, Randy J. ; Miller, Sara E. ; Perriard, Jean Claude ; Gavin, Timothy P. ; Hickner, Robert C. ; Coffman, Thomas M. ; Somlyo, Avril V. ; Yan, Zhen ; Haystead, Timothy A J. / Deletion of the protein kinase A/protein kinase G target SMTNL1 promotes an exercise-adapted phenotype in vascular smooth muscle. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 17. pp. 11850-11859.
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AU - Fortner, Christopher N.

AU - Lontay, Beata

AU - Akimoto, Takayuki

AU - Neppl, Ronald L.

AU - Facemire, Carie

AU - Datto, Michael B.

AU - Kwon, Ashley

AU - McCook, Everett

AU - Li, Ping

AU - Wang, Shiliang

AU - Thresher, Randy J.

AU - Miller, Sara E.

AU - Perriard, Jean Claude

AU - Gavin, Timothy P.

AU - Hickner, Robert C.

AU - Coffman, Thomas M.

AU - Somlyo, Avril V.

AU - Yan, Zhen

AU - Haystead, Timothy A J

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N2 - In vivo protein kinases A and G (PKA and PKG) coordinately phosphorylate a broad range of substrates to mediate their various physiological effects. The functions of many of these substrates have yet to be defined genetically. Herein we show a role for smoothelin-like protein 1 (SMTNL1), a novel in vivo target of PKG/PKA, in mediating vascular adaptations to exercise. Aortas from smtnl1-/- mice exhibited strikingly enhanced vasorelaxation before exercise, similar in extent to that achieved after endurance training of wild-type littermates. Additionally, contractile responses to α-adrenergic agonists were greatly attenuated. Immunological studies showed SMTNL1 is expressed in smooth muscle and type 2a striated muscle fibers. Consistent with a role in adaptations to exercise, smtnl1-/- mice also exhibited increased type 2a fibers before training and better performance after forced endurance training compared smtnl1+/+ mice. Furthermore, exercise was found to reduce expression of SMTNL1, particularly in female mice. In both muscle types, SMTNL1 is phosphorylated at Ser-301 in response to adrenergic signals. In vitro SMTNL1 suppresses myosin phosphatase activity through a substrate-directed effect, which is relieved by Ser-301 phosphorylation. Our findings suggest roles for SMTNL1 in cGMP/cAMP-mediated adaptations to exercise through mechanisms involving direct modulation of contractile activity.

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