Deoxyribonucleic acid concentration using acoustic radiation force

Kenji Yasuda, M. Kiyama, S. I. Umemura, K. Takeda

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A deoxyribonucleic acid (DNA) concentration method using acoustic radiation force is proposed. Ethanol was added to an aqueous DNA sample in order to initiate clustering of the DNA molecules of 2686 bps. The change in the distribution of the DNA clusters induced by u standing wave ultrasound irradiation was observed with an optical microscope. The observable DNA clusters gathered on the pressure node in the ultrasonic standing wave field and formed larger clusters that did not break apart after the irradiation was stopped. Without ultrasound irradiation, no significant change in the cluster distribution was observed. This method may have a potential use for concentrating and collecting biomaterials such as nucleic acids and proteins.

Original languageEnglish
Pages (from-to)1248-1251
Number of pages4
JournalJournal of the Acoustical Society of America
Volume99
Issue number2
DOIs
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

sound waves
deoxyribonucleic acid
standing waves
irradiation
concentrating
nucleic acids
optical microscopes
ethyl alcohol
ultrasonics
Radiation
Acoustics
proteins
molecules
Ultrasound
Waves

ASJC Scopus subject areas

  • Acoustics and Ultrasonics

Cite this

Deoxyribonucleic acid concentration using acoustic radiation force. / Yasuda, Kenji; Kiyama, M.; Umemura, S. I.; Takeda, K.

In: Journal of the Acoustical Society of America, Vol. 99, No. 2, 1996, p. 1248-1251.

Research output: Contribution to journalArticle

Yasuda, Kenji ; Kiyama, M. ; Umemura, S. I. ; Takeda, K. / Deoxyribonucleic acid concentration using acoustic radiation force. In: Journal of the Acoustical Society of America. 1996 ; Vol. 99, No. 2. pp. 1248-1251.
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