Deoxyribonucleic-acid-sensitive polycrystalline diamond solutiongate field-effect transistor with a carboxyl-terminated borondoped channel

Yukihiro Shintani, Shoji Ibori, Hiroshi Kawarada

Research output: Contribution to journalArticle

Abstract

This paper describes a deoxyribonucleic-acid-sensitive electrolyte solution-gate field-effect transistor (SGFET) sensor utilizing a partial carboxyl-terminated boron-doped polycrystalline diamond surface as a linker to connect a deoxyribonucleic acid (DNA) probe. A high density of carboxyl termination on the polycrystalline diamond surface that was employed as a FET channel was achieved using a vacuum ultraviolet system with oxygen gas. A single-stranded DNA probe was immobilized on the polycrystalline diamond channel via amino coupling. The current-voltage characteristics of the polycrystalline diamond SGFET sensor was examined with bias voltages within its potential voltage window. The characteristics of the drain-source current verses the drain-source voltage showed a pinch-off, a shift voltage of up to 40 mV with a coefficient of variation of 4 - 11% was obtained between hybridization and denaturation. In addition, a single nucleotide mutation of DNA sequence was selectively recognized by the shift voltage up to ca. 10 mV.

Original languageEnglish
Pages (from-to)923-927
Number of pages5
JournalAnalytical Sciences
Volume35
Issue number8
DOIs
Publication statusPublished - 2019 Jan 1

Keywords

  • Boron-doped diamond
  • Carboxyl-termination
  • Deoxyribonucleic acid
  • Electrolyte-solution-gate field-effect transistor
  • Polycrystalline diamond

ASJC Scopus subject areas

  • Analytical Chemistry

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