Dependence of protein binding capacity of dimethylamino-γ-butyric-acid (DMGABA)-immobilized porous membrane on composition of solvent used for DMGABA immobilization

Akio Iwanade*, Daisuke Umeno, Kyoichi Saito, Takanobu Sugo

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Dimethylamino-γ-butyric acid (DMGABA) as an ampholite was reacted with the epoxy group of the poly-glycidyl methacrylate chain grafted onto the pore surface of a porous hollow-fiber polyethylene membrane by radiation-induced graft polymerization. DMGABA was dissolved in a mixture of dioxane and water at various dioxane volume fractions, defined by dividing the dioxane volume by the total volume. The equilibrium binding capacity (EBC) of the DMGABA-immobilized porous hollow-fiber membrane for lysozyme was evaluated in the permeation mode. The EBC was varied from a 1/50-fold monolayer binding capacity to a 10-fold monolayer binding capacity by controlling the composition of the solvent used for DMGABA immobilization and the molar conversion of the epoxy group into the DMGABA group.

Original languageEnglish
Pages (from-to)53-58
Number of pages6
JournalRadiation Physics and Chemistry
Volume87
DOIs
Publication statusPublished - 2013 Jun
Externally publishedYes

Keywords

  • Dimethylamino-γ-butyric acid
  • Immobilization
  • Porous hollow-fiber membrane
  • Protein adsorption
  • Solvent composition

ASJC Scopus subject areas

  • Radiation

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