Abstract
To elucidate the role of the community effect in cardiomyocytes, we developed an on-chip single-cell-based culture system with which the dynamics of the change of beat rate and beat rate fluctuation of cultured cardiomyocytes can be measured at the single-cell level before and after the formation of a cell network. We examined the dependence of the community effect on cell network pattern by culturing cardiomyocytes in differently shaped microchambers and investigated the relation between the network pattern and the stability of the beat rate. We found that beat rate fluctuation tends to decrease as cell-community size increases, irrespective of cell network pattern. This indicates that on-chip single-cell-based cardiomyocyte communities might be able to model a heart tissue accurately enough to be used in practical applications such as drug screening.
| Original language | English |
|---|---|
| Pages (from-to) | 494-498 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 356 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2007 May 4 |
| Externally published | Yes |
Fingerprint
Keywords
- Beat rate fluctuation
- Cell network pattern
- Community effect
- Cultured cardiomyocyte
- Heart tissue model
- On-chip single-cell-based culture system
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Dependence of the community effect of cultured cardiomyocytes on the cell network pattern. / Kaneko, Tomoyuki; Kojima, Kensuke; Yasuda, Kenji.
In: Biochemical and Biophysical Research Communications, Vol. 356, No. 2, 04.05.2007, p. 494-498.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dependence of the community effect of cultured cardiomyocytes on the cell network pattern
AU - Kaneko, Tomoyuki
AU - Kojima, Kensuke
AU - Yasuda, Kenji
PY - 2007/5/4
Y1 - 2007/5/4
N2 - To elucidate the role of the community effect in cardiomyocytes, we developed an on-chip single-cell-based culture system with which the dynamics of the change of beat rate and beat rate fluctuation of cultured cardiomyocytes can be measured at the single-cell level before and after the formation of a cell network. We examined the dependence of the community effect on cell network pattern by culturing cardiomyocytes in differently shaped microchambers and investigated the relation between the network pattern and the stability of the beat rate. We found that beat rate fluctuation tends to decrease as cell-community size increases, irrespective of cell network pattern. This indicates that on-chip single-cell-based cardiomyocyte communities might be able to model a heart tissue accurately enough to be used in practical applications such as drug screening.
AB - To elucidate the role of the community effect in cardiomyocytes, we developed an on-chip single-cell-based culture system with which the dynamics of the change of beat rate and beat rate fluctuation of cultured cardiomyocytes can be measured at the single-cell level before and after the formation of a cell network. We examined the dependence of the community effect on cell network pattern by culturing cardiomyocytes in differently shaped microchambers and investigated the relation between the network pattern and the stability of the beat rate. We found that beat rate fluctuation tends to decrease as cell-community size increases, irrespective of cell network pattern. This indicates that on-chip single-cell-based cardiomyocyte communities might be able to model a heart tissue accurately enough to be used in practical applications such as drug screening.
KW - Beat rate fluctuation
KW - Cell network pattern
KW - Community effect
KW - Cultured cardiomyocyte
KW - Heart tissue model
KW - On-chip single-cell-based culture system
UR - http://www.scopus.com/inward/record.url?scp=33947431669&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33947431669&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2007.03.005
DO - 10.1016/j.bbrc.2007.03.005
M3 - Article
C2 - 17359936
AN - SCOPUS:33947431669
VL - 356
SP - 494
EP - 498
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -