Design and application of a new cryptic-plasmid-based shuttle vector for Magnetospirillum magneticum

Yoshiko Okamura, Haruko Takeyama, Takumi Sekine, Toshifumi Sakaguchi, Aris Tri Wahyudi, Rika Sato, Shinji Kamiya, Tadashi Matsunaga

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A 3.7-kb cryptic plasmid designated pMGT was found in Magnetospirillum magneticum MGT-1. It was characterized and used for the development of an improved expression system in strain AMB-1 through the construction of a shuttle vector, pUMG. An electroporation method for magnetic bacteria that uses the cryptic plasmid was also developed.

Original languageEnglish
Pages (from-to)4274-4277
Number of pages4
JournalApplied and Environmental Microbiology
Volume69
Issue number7
DOIs
Publication statusPublished - 2003 Jul 1
Externally publishedYes

Fingerprint

Magnetospirillum
genetic vectors
Genetic Vectors
plasmid
plasmids
Plasmids
Electroporation
electroporation
Bacteria
bacterium
bacteria
methodology

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Design and application of a new cryptic-plasmid-based shuttle vector for Magnetospirillum magneticum. / Okamura, Yoshiko; Takeyama, Haruko; Sekine, Takumi; Sakaguchi, Toshifumi; Wahyudi, Aris Tri; Sato, Rika; Kamiya, Shinji; Matsunaga, Tadashi.

In: Applied and Environmental Microbiology, Vol. 69, No. 7, 01.07.2003, p. 4274-4277.

Research output: Contribution to journalArticle

Okamura, Yoshiko ; Takeyama, Haruko ; Sekine, Takumi ; Sakaguchi, Toshifumi ; Wahyudi, Aris Tri ; Sato, Rika ; Kamiya, Shinji ; Matsunaga, Tadashi. / Design and application of a new cryptic-plasmid-based shuttle vector for Magnetospirillum magneticum. In: Applied and Environmental Microbiology. 2003 ; Vol. 69, No. 7. pp. 4274-4277.
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