Design and application of a new cryptic-plasmid-based shuttle vector for Magnetospirillum magneticum

Yoshiko Okamura, Haruko Takeyama, Takumi Sekine, Toshifumi Sakaguchi, Aris Tri Wahyudi, Rika Sato, Shinji Kamiya, Tadashi Matsunaga

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

A 3.7-kb cryptic plasmid designated pMGT was found in Magnetospirillum magneticum MGT-1. It was characterized and used for the development of an improved expression system in strain AMB-1 through the construction of a shuttle vector, pUMG. An electroporation method for magnetic bacteria that uses the cryptic plasmid was also developed.

Original languageEnglish
Pages (from-to)4274-4277
Number of pages4
JournalApplied and Environmental Microbiology
Volume69
Issue number7
DOIs
Publication statusPublished - 2003 Jul 1

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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