Design and in vitro activity of ribozymes against mRNA of a rat membrane inhibitor of complement.

O. Inui, T. Tanaka, N. Dohi, N. Okada, H. Okada, Yo Kikuchi

Research output: Contribution to journalArticle

Abstract

Mammalian cells are usually protected from the complement-mediated injury by a number of complement regulatory proteins. A rat protein designated as 512 antigen is thought to be such a complement regulatory protein. A cDNA of 512 antigen has been cloned and analyzed. To investigate the function of 512 antigen, we plan to construct a ribozyme system against 512 antigen expression. We have designed two hammerhead ribozymes targeted to the 512 antigen mRNA and tested the ribozyme activities in vitro. Both hammerhead ribozymes efficiently cleaved the 512 antigen mRNA in vitro. We have also designed a hairpin ribozyme against this mRNA. The activity of the hairpin ribozyme will also be discussed.

Original languageEnglish
Pages (from-to)223-224
Number of pages2
JournalNucleic acids symposium series
Issue number37
Publication statusPublished - 1997
Externally publishedYes

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    Inui, O., Tanaka, T., Dohi, N., Okada, N., Okada, H., & Kikuchi, Y. (1997). Design and in vitro activity of ribozymes against mRNA of a rat membrane inhibitor of complement. Nucleic acids symposium series, (37), 223-224.