Design and test of filter of high gradient magnetic separation system for trapping immunoglobulin in serum

Hiroshi Ueda, Koh Agatsuma, Kazuhiro Kajikawa, Mitsuho Furuse, Shuichiro Fuchino, Atsushi Ishiyama

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Recently, affinity magnetic beads have been widely used in immunomagnetic cell sorting (IMCS) technology. Today, we can easily sort and analyze DNA and antibodies (immunoglob- ulin) using various types of affinity magnetic beads available in the market. The diameters of these affinity magnetic beads used in immunomagnetic cell sorting are limited to above approximately 1 /xm because of the low magnetic fields induced by permanent magnets. Now, nano-sized affinity magnetic beads are strongly desired to achieve high resolutions. We have been studying and attempting to develop a high-gradient magnetic separation (HGMS) system that employs a superconducting magnet to induce a considerably higher magnetic field than that induced by a permanent magnet in order to trap smaller nano-sized affinity magnetic beads by a filter made of fine stainless steel wool. In this study, we constructed a prototype of a desktop-type HGMS system using a cryocooler-cooled LTS magnet and conducted preliminary experiments on trapping the nano-sized magnetic particles. Furthermore, we investigated the magnetic field distribution and magnetic force around a magnetic wire in the filter by means of a numerical simulation.

    Original languageEnglish
    Article number5109581
    Pages (from-to)2157-2161
    Number of pages5
    JournalIEEE Transactions on Applied Superconductivity
    Volume19
    Issue number3
    DOIs
    Publication statusPublished - 2009 Jun

    Fingerprint

    Magnetic separation
    beads
    serums
    affinity
    Immunoglobulins
    trapping
    Magnetic fields
    Sorting
    filters
    gradients
    Permanent magnets
    Cells
    classifying
    permanent magnets
    Superconducting magnets
    Stainless Steel
    Wool
    Antibodies
    Magnets
    magnetic fields

    Keywords

    • High-gradient magnetic separation
    • Immunomagnetic cell sorting
    • Nano-sized magnetic particles

    ASJC Scopus subject areas

    • Electrical and Electronic Engineering
    • Condensed Matter Physics
    • Electronic, Optical and Magnetic Materials

    Cite this

    Design and test of filter of high gradient magnetic separation system for trapping immunoglobulin in serum. / Ueda, Hiroshi; Agatsuma, Koh; Kajikawa, Kazuhiro; Furuse, Mitsuho; Fuchino, Shuichiro; Ishiyama, Atsushi.

    In: IEEE Transactions on Applied Superconductivity, Vol. 19, No. 3, 5109581, 06.2009, p. 2157-2161.

    Research output: Contribution to journalArticle

    Ueda, Hiroshi ; Agatsuma, Koh ; Kajikawa, Kazuhiro ; Furuse, Mitsuho ; Fuchino, Shuichiro ; Ishiyama, Atsushi. / Design and test of filter of high gradient magnetic separation system for trapping immunoglobulin in serum. In: IEEE Transactions on Applied Superconductivity. 2009 ; Vol. 19, No. 3. pp. 2157-2161.
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    AU - Fuchino, Shuichiro

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