Design of 16S rRNA-targeted oligonucleotide probes and microbial community analysis in the denitrification process of a saline industrial wastewater treatment system

Sachiko Yoshie, Naohiro Noda, Satoshi Tsuneda, Akira Hirata, Yuhei Inamori

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    Three 16S rRNA-targeted oligonucleotide probes, namely, PSMg437 targeting several members of the genus Pseudomonas, Hlm474 targeting several members of the genus Halomonas, and Clw844 targeting several members of the genus Colwellia, were designed. The microbial community structure and nitrogen removal ability of nitrate-containing saline wastewater treatment systems with anaerobic packed bed and fluidized bed were monitored. Direct cell counting using fluorescence in situ hybridization (FISH) images revealed that various phylogenetic groups were evenly distributed in the anaerobic packed bed whereas members of the genus Halomonas were dominant particularly in the anaerobic fluidized bed. These results suggest that the microbial communities produced by different flow conditions correlated with denitrification ability in saline industrial wastewater treatment systems.

    Original languageEnglish
    Pages (from-to)183-189
    Number of pages7
    JournalFEMS Microbiology Letters
    Volume235
    Issue number1
    DOIs
    Publication statusPublished - 2004 Jun 1

    Fingerprint

    Halomonas
    Denitrification
    Oligonucleotide Probes
    Waste Water
    Alteromonadaceae
    Pseudomonas
    Fluorescence In Situ Hybridization
    Nitrates
    Nitrogen

    Keywords

    • Colwellia
    • Denitrification
    • Fluorescence in situ hybridization
    • Halomonas
    • Saline industrial wastewater

    ASJC Scopus subject areas

    • Genetics
    • Molecular Biology
    • Applied Microbiology and Biotechnology
    • Microbiology

    Cite this

    Design of 16S rRNA-targeted oligonucleotide probes and microbial community analysis in the denitrification process of a saline industrial wastewater treatment system. / Yoshie, Sachiko; Noda, Naohiro; Tsuneda, Satoshi; Hirata, Akira; Inamori, Yuhei.

    In: FEMS Microbiology Letters, Vol. 235, No. 1, 01.06.2004, p. 183-189.

    Research output: Contribution to journalArticle

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