Design of a sedimentation hole in a microfluidic channel to remove blood cells from diluted whole blood

Chiaki Kuroda, Yoshimichi Ohki, Hiroki Ashiba, Makoto Fujimaki, Koichi Awazu, Makoto Makishima

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    Abstract

    With the aim of developing a sensor for rapidly detecting viruses in a drop of blood, in this study, we analyze the shape of a hole in a microfluidic channel in relation to the efficiency of sedimentation of blood cells. The efficiency of sedimentation is examined on the basis of our calculation and experimental results for two types of sedimentation hole, cylindrical and truncated conical holes, focusing on the Boycott effect, which can promote the sedimentation of blood cells from a downward-facing wall. As a result, we demonstrated that blood cells can be eliminated with an efficiency of 99% or higher by retaining a diluted blood sample of about 30 μL in the conical hole for only 2 min. Moreover, we succeeded in detecting the antihepatitis B surface antigen antibody in blood using a waveguide-mode sensor equipped with a microfluidic channel having the conical sedimentation hole.

    Original languageEnglish
    Article number037201
    JournalJapanese Journal of Applied Physics
    Volume56
    Issue number3
    DOIs
    Publication statusPublished - 2017 Mar 1

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    ASJC Scopus subject areas

    • Engineering(all)
    • Physics and Astronomy(all)

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