Design of a sedimentation hole in a microfluidic channel to remove blood cells from diluted whole blood

Chiaki Kuroda, Yoshimichi Ohki, Hiroki Ashiba, Makoto Fujimaki, Koichi Awazu, Makoto Makishima

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    With the aim of developing a sensor for rapidly detecting viruses in a drop of blood, in this study, we analyze the shape of a hole in a microfluidic channel in relation to the efficiency of sedimentation of blood cells. The efficiency of sedimentation is examined on the basis of our calculation and experimental results for two types of sedimentation hole, cylindrical and truncated conical holes, focusing on the Boycott effect, which can promote the sedimentation of blood cells from a downward-facing wall. As a result, we demonstrated that blood cells can be eliminated with an efficiency of 99% or higher by retaining a diluted blood sample of about 30 μL in the conical hole for only 2 min. Moreover, we succeeded in detecting the antihepatitis B surface antigen antibody in blood using a waveguide-mode sensor equipped with a microfluidic channel having the conical sedimentation hole.

    Original languageEnglish
    Article number037201
    JournalJapanese Journal of Applied Physics
    Volume56
    Issue number3
    DOIs
    Publication statusPublished - 2017 Mar 1

    Fingerprint

    blood cells
    Sedimentation
    Microfluidics
    blood
    Blood
    Cells
    sensors
    viruses
    antigens
    Facings
    retaining
    antibodies
    Sensors
    Antigens
    Viruses
    Antibodies
    waveguides
    Waveguides

    ASJC Scopus subject areas

    • Engineering(all)
    • Physics and Astronomy(all)

    Cite this

    Design of a sedimentation hole in a microfluidic channel to remove blood cells from diluted whole blood. / Kuroda, Chiaki; Ohki, Yoshimichi; Ashiba, Hiroki; Fujimaki, Makoto; Awazu, Koichi; Makishima, Makoto.

    In: Japanese Journal of Applied Physics, Vol. 56, No. 3, 037201, 01.03.2017.

    Research output: Contribution to journalArticle

    Kuroda, Chiaki ; Ohki, Yoshimichi ; Ashiba, Hiroki ; Fujimaki, Makoto ; Awazu, Koichi ; Makishima, Makoto. / Design of a sedimentation hole in a microfluidic channel to remove blood cells from diluted whole blood. In: Japanese Journal of Applied Physics. 2017 ; Vol. 56, No. 3.
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