TY - JOUR
T1 - Design of polymer brushes for immobilizing enzymes onto hollow fiber micropores in organic media reaction
AU - Goto, Muneharu
AU - Okubo, Tokie
AU - Kawakita, Hidetaka
AU - Uezu, Kazuya
AU - Tsuneda, Satoshi
AU - Saito, Kyoichi
AU - Goto, Masahiro
AU - Tamada, Masao
AU - Sugo, Takanobu
PY - 2007/11/15
Y1 - 2007/11/15
N2 - To immobilize lipase for enzymatic reactions in organic solvent, various functional [epoxy (GMA-fiber), hydroxyl (OH-fiber) or diethyl amino (DEA-fiber)] groups were introduced onto porous hollow-fiber membranes by radiation-induced graft polymerization of glycidyl methacrylate and chemical modification. Lipase from Candida rugosa was immobilized on polymer brushes by permeation of lipase. The activities of immobilized lipase were measured by esterification reactions between lauric acid and benzyl alcohol in isooctane. The activity of immobilized lipase on GMA-fibers, DEA-fibers and OH-fibers was 0.70 mol/(h kg-lipase), 0.50 mol/(h kg-lipase), and 2.45 mol/(h kg-lipase), respectively. Immobilized lipase on DEA-fibers or OH-fibers was reused three times after it was used in the batch reactor for 24 h. It was found that lipase activity showed no signs of denaturation. However, when native lipase was used, lipase activity remarkably decreased after reusing.
AB - To immobilize lipase for enzymatic reactions in organic solvent, various functional [epoxy (GMA-fiber), hydroxyl (OH-fiber) or diethyl amino (DEA-fiber)] groups were introduced onto porous hollow-fiber membranes by radiation-induced graft polymerization of glycidyl methacrylate and chemical modification. Lipase from Candida rugosa was immobilized on polymer brushes by permeation of lipase. The activities of immobilized lipase were measured by esterification reactions between lauric acid and benzyl alcohol in isooctane. The activity of immobilized lipase on GMA-fibers, DEA-fibers and OH-fibers was 0.70 mol/(h kg-lipase), 0.50 mol/(h kg-lipase), and 2.45 mol/(h kg-lipase), respectively. Immobilized lipase on DEA-fibers or OH-fibers was reused three times after it was used in the batch reactor for 24 h. It was found that lipase activity showed no signs of denaturation. However, when native lipase was used, lipase activity remarkably decreased after reusing.
KW - Enzyme activity
KW - Enzyme technology
KW - Esterification reaction
KW - Immobilized enzymes
KW - Lipase
KW - Polymer brush
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U2 - 10.1016/j.bej.2007.04.008
DO - 10.1016/j.bej.2007.04.008
M3 - Article
AN - SCOPUS:35248852814
SN - 1369-703X
VL - 37
SP - 159
EP - 165
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
IS - 2
ER -