Desmutagenic and bio-antimutagenic activity of docosahexaenoic acid and eicosapentaenoic acid in cultured Chinese hamster V79 cells

Yukiaki Kuroda, Naoko Shima, Kazunaga Yazawa, Kazuhiko Kaji

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The antimutagenic activities of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were examined by studying their effects on induction of 6-thioguanine (6TG)-resistant mutations by ethyl methanesulfonate (EMS) in cultured Chinese hamster V79 cells. DRA had a remarkable inhibitory effect against the cytotoxicity of EMS, when cells were simultaneously-treated with EMS, showing a blocking or scavenging activity of DHA in reduction of surviving fraction of cells. DHA had not so significant effect, when cells were treated before and after treatment with EMS. On the other hand, EPA had marked inhibiting effects against cytotoxicity of EMS, when cells were treated with EPA, before, simultaneous and after treatment with EMS. Against the induction of mutations by EMS, an antimutagenic activity of DHA was found when cells were pre-treated, simultaneously-treated or post-treated with DHA. EPA was also effective in reducing EMS-induced 6TG-resistant mutations when the cells were treated using the three different treatment procedures described above. The results suggest that in cultured Chinese hamster V79 cells, DHA and EPA may have both desmutagenic activity, which inactivates EMS chemically and/or enzymatically and bio-antimutagenic activity which suppresses mutation fixation after DNA is damaged by EMS.

Original languageEnglish
Pages (from-to)123-130
Number of pages8
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume497
Issue number1-2
DOIs
Publication statusPublished - 2001 Oct 18
Externally publishedYes

Fingerprint

Ethyl Methanesulfonate
Eicosapentaenoic Acid
Docosahexaenoic Acids
Cricetulus
Thioguanine
Mutation

Keywords

  • 6TG-resistant mutation
  • Bio-antimutagen
  • Chinese hamster V79 cells
  • Desmutagen
  • DHA
  • EPA

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Genetics

Cite this

Desmutagenic and bio-antimutagenic activity of docosahexaenoic acid and eicosapentaenoic acid in cultured Chinese hamster V79 cells. / Kuroda, Yukiaki; Shima, Naoko; Yazawa, Kazunaga; Kaji, Kazuhiko.

In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 497, No. 1-2, 18.10.2001, p. 123-130.

Research output: Contribution to journalArticle

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