Destruction and reconstitution of polyphospholipid vesicles with size memory

Shinji Takeoka, Takeru Ohgushi, Eishun Tsuchida

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    1,2-Bis(2,4-octadecadienoyl)-sn-glycero-3-phosphocholine (DODPC), constructing the bilayer membrane of a vesicle, was polymerized by UV irradiation. The freeze-dried vesicles were completely dissolved in chloroform to obtain cross-linked phospholipid polymer (polyDODPC). The polyDODPC consisted of high molecular weight components and oligomers. The polyDODPC was dispersed by sonication, and two size distributions of the reconstituted vesicles were confirmed, larger vesicles from high molecular weight polyDODPC and smaller vesicles from oligomers. When the polyDODPC vesicles of various size and unimodal size distribution were reconstituted by an extrusion method, the vesicles of the same size as the vesicles at polymerization showed excellent stability compared with the other sizes. Interestingly, the original size of the polyDODPC vesicles could be completely restored by a cholate dialysis method. It was also clarified that vesicles larger than the original size were reconstituted from the higher molecular weight component, and the smaller vesicles were from the oligomers. Furthermore, a linear phospholipid polymer obtained from 1-palmitoyl-2-(2,4- octadecadienoyl)-sn-glycero-3-phosphocholine could not restore the original size. Those results indicate that the original size of the vesicles can be restored completely by all polyDODPCs having the original composition of large and small molecular weights and cross-linked structure.

    Original languageEnglish
    Pages (from-to)7660-7666
    Number of pages7
    JournalMacromolecules
    Volume28
    Issue number23
    Publication statusPublished - 1995 Nov 6

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    Molecular weight
    Oligomers
    Data storage equipment
    Phospholipids
    Polymers
    Cholates
    Sonication
    Phosphorylcholine
    Dialysis
    Chloroform
    Chlorine compounds
    Extrusion
    Polymerization
    Irradiation
    Membranes
    Chemical analysis

    ASJC Scopus subject areas

    • Materials Chemistry

    Cite this

    Destruction and reconstitution of polyphospholipid vesicles with size memory. / Takeoka, Shinji; Ohgushi, Takeru; Tsuchida, Eishun.

    In: Macromolecules, Vol. 28, No. 23, 06.11.1995, p. 7660-7666.

    Research output: Contribution to journalArticle

    Takeoka, S, Ohgushi, T & Tsuchida, E 1995, 'Destruction and reconstitution of polyphospholipid vesicles with size memory', Macromolecules, vol. 28, no. 23, pp. 7660-7666.
    Takeoka, Shinji ; Ohgushi, Takeru ; Tsuchida, Eishun. / Destruction and reconstitution of polyphospholipid vesicles with size memory. In: Macromolecules. 1995 ; Vol. 28, No. 23. pp. 7660-7666.
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