Destruction of organelle nuclei during spermatogenesis in Chara corallina examined by staining with DAPI and anti-DNA antibody

Ge Hong Sun, Taro Q.P. Uyeda, Tsuneyoshi Kuroiwa

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

The behavior of plastid and mitochondrial nuclei (synonymous with nucleoids) during spermatogenesis in Chara corallina was examined by fluorescence microscopy after staining with 4′,6-diamidino-2-phenylindole (DAPI). These organelle nuclei, which were present in internode cells and cells at the early spermatid stage, disappeared during spermatogenesis. This conclusion was confirmed by immunofluorescence microscopy using of a monoclonal anti-DNA antibody. The pattern of fluorescence obtained using the antibody coincided with that obtained by staining with DAPI. These results suggest that the disappearance of nuclei from male-derived organelles is most likely to result in maternal inheritance in Chara corallina.

Original languageEnglish
Pages (from-to)185-188
Number of pages4
JournalProtoplasma
Volume144
Issue number2-3
DOIs
Publication statusPublished - 1988 Jun
Externally publishedYes

Keywords

  • 4′,6-diamidino-2-phenylindole (DAPI)
  • Anti-DNA antibody
  • Chara corallina
  • Maternal inheritance
  • Mitochondrial nuclei
  • Organelle nucleus
  • Plastid nuclei

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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