Destruction of organelle nuclei during spermatogenesis in Chara corallina examined by staining with DAPI and anti-DNA antibody

Ge Hong Sun; Taro Q.P. Uyeda; Tsuneyoshi Kuroiwa

doi:10.1007/BF01637252

Destruction of organelle nuclei during spermatogenesis in Chara corallina examined by staining with DAPI and anti-DNA antibody

Ge Hong Sun, Taro Q.P. Uyeda, Tsuneyoshi Kuroiwa^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

The behavior of plastid and mitochondrial nuclei (synonymous with nucleoids) during spermatogenesis in Chara corallina was examined by fluorescence microscopy after staining with 4′,6-diamidino-2-phenylindole (DAPI). These organelle nuclei, which were present in internode cells and cells at the early spermatid stage, disappeared during spermatogenesis. This conclusion was confirmed by immunofluorescence microscopy using of a monoclonal anti-DNA antibody. The pattern of fluorescence obtained using the antibody coincided with that obtained by staining with DAPI. These results suggest that the disappearance of nuclei from male-derived organelles is most likely to result in maternal inheritance in Chara corallina.

Original language	English
Pages (from-to)	185-188
Number of pages	4
Journal	Protoplasma
Volume	144
Issue number	2-3
DOIs	https://doi.org/10.1007/BF01637252
Publication status	Published - 1988 Jun
Externally published	Yes

Keywords

4′,6-diamidino-2-phenylindole (DAPI)
Anti-DNA antibody
Chara corallina
Maternal inheritance
Mitochondrial nuclei
Organelle nucleus
Plastid nuclei

ASJC Scopus subject areas

Plant Science
Cell Biology

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10.1007/BF01637252

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@article{7116ca1514a2468280ba74429500674d,

title = "Destruction of organelle nuclei during spermatogenesis in Chara corallina examined by staining with DAPI and anti-DNA antibody",

abstract = "The behavior of plastid and mitochondrial nuclei (synonymous with nucleoids) during spermatogenesis in Chara corallina was examined by fluorescence microscopy after staining with 4′,6-diamidino-2-phenylindole (DAPI). These organelle nuclei, which were present in internode cells and cells at the early spermatid stage, disappeared during spermatogenesis. This conclusion was confirmed by immunofluorescence microscopy using of a monoclonal anti-DNA antibody. The pattern of fluorescence obtained using the antibody coincided with that obtained by staining with DAPI. These results suggest that the disappearance of nuclei from male-derived organelles is most likely to result in maternal inheritance in Chara corallina.",

keywords = "4′,6-diamidino-2-phenylindole (DAPI), Anti-DNA antibody, Chara corallina, Maternal inheritance, Mitochondrial nuclei, Organelle nucleus, Plastid nuclei",

author = "Sun, {Ge Hong} and Uyeda, {Taro Q.P.} and Tsuneyoshi Kuroiwa",

year = "1988",

month = jun,

doi = "10.1007/BF01637252",

language = "English",

volume = "144",

pages = "185--188",

journal = "Protoplasma",

issn = "0033-183X",

publisher = "Springer Wien",

number = "2-3",

}

TY - JOUR

T1 - Destruction of organelle nuclei during spermatogenesis in Chara corallina examined by staining with DAPI and anti-DNA antibody

AU - Sun, Ge Hong

AU - Uyeda, Taro Q.P.

AU - Kuroiwa, Tsuneyoshi

PY - 1988/6

Y1 - 1988/6

N2 - The behavior of plastid and mitochondrial nuclei (synonymous with nucleoids) during spermatogenesis in Chara corallina was examined by fluorescence microscopy after staining with 4′,6-diamidino-2-phenylindole (DAPI). These organelle nuclei, which were present in internode cells and cells at the early spermatid stage, disappeared during spermatogenesis. This conclusion was confirmed by immunofluorescence microscopy using of a monoclonal anti-DNA antibody. The pattern of fluorescence obtained using the antibody coincided with that obtained by staining with DAPI. These results suggest that the disappearance of nuclei from male-derived organelles is most likely to result in maternal inheritance in Chara corallina.

AB - The behavior of plastid and mitochondrial nuclei (synonymous with nucleoids) during spermatogenesis in Chara corallina was examined by fluorescence microscopy after staining with 4′,6-diamidino-2-phenylindole (DAPI). These organelle nuclei, which were present in internode cells and cells at the early spermatid stage, disappeared during spermatogenesis. This conclusion was confirmed by immunofluorescence microscopy using of a monoclonal anti-DNA antibody. The pattern of fluorescence obtained using the antibody coincided with that obtained by staining with DAPI. These results suggest that the disappearance of nuclei from male-derived organelles is most likely to result in maternal inheritance in Chara corallina.

KW - 4′,6-diamidino-2-phenylindole (DAPI)

KW - Anti-DNA antibody

KW - Chara corallina

KW - Maternal inheritance

KW - Mitochondrial nuclei

KW - Organelle nucleus

KW - Plastid nuclei

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DO - 10.1007/BF01637252

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SP - 185

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JO - Protoplasma

JF - Protoplasma

IS - 2-3

ER -

Destruction of organelle nuclei during spermatogenesis in Chara corallina examined by staining with DAPI and anti-DNA antibody

Abstract

Keywords

ASJC Scopus subject areas

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