Destruction of organelle nuclei during spermatogenesis in Chara corallina examined by staining with DAPI and anti-DNA antibody

Ge Hong Sun, Taro Q.P. Uyeda, Tsuneyoshi Kuroiwa

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The behavior of plastid and mitochondrial nuclei (synonymous with nucleoids) during spermatogenesis in Chara corallina was examined by fluorescence microscopy after staining with 4′,6-diamidino-2-phenylindole (DAPI). These organelle nuclei, which were present in internode cells and cells at the early spermatid stage, disappeared during spermatogenesis. This conclusion was confirmed by immunofluorescence microscopy using of a monoclonal anti-DNA antibody. The pattern of fluorescence obtained using the antibody coincided with that obtained by staining with DAPI. These results suggest that the disappearance of nuclei from male-derived organelles is most likely to result in maternal inheritance in Chara corallina.

Original languageEnglish
Pages (from-to)185-188
Number of pages4
JournalProtoplasma
Volume144
Issue number2-3
DOIs
Publication statusPublished - 1988 Jun
Externally publishedYes

Keywords

  • 4′,6-diamidino-2-phenylindole (DAPI)
  • Anti-DNA antibody
  • Chara corallina
  • Maternal inheritance
  • Mitochondrial nuclei
  • Organelle nucleus
  • Plastid nuclei

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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