Abstract
An optrode system based on a competitive binding technique for fluoroimmuoassay was developed. Immunoglobulin E (IgE) was immobilized on a membrane which covered the edge of an optical fiber. The sensor was exposed to a solution containing dinitrophenylated bovine serum albumin (DNP-BSA) and then allophycocyanin conjugated DNP-BSA was added. A helium-neon (He-Ne) laser provided an excitation of the binding antigen-antibody complex. The resulting in fluorescence emission was measured using the sensing probe. The fluorescence intensity was proportional to the amount of allophycocyanin conjugated DNP-BSA in the sample. A linear relationship was obtained between the relative fluorescence intensity and the allergen concentration in the range 0~10 ng ml–1.
Original language | English |
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Pages (from-to) | 761-765 |
Number of pages | 5 |
Journal | Bunseki Kagaku |
Volume | 39 |
Issue number | 11 |
DOIs | |
Publication status | Published - 1990 |
Externally published | Yes |
Keywords
- detection of allergen
- fluoroimmunoassay
- immunoglobulin E
- optrode sensor system
ASJC Scopus subject areas
- Analytical Chemistry