Abstract
An optrode system based on a competitive binding technique for fluoroimmuoassay was developed. Immunoglobulin E (IgE) was immobilized on a membrane which covered the edge of an optical fiber. The sensor was exposed to a solution containing dinitrophenylated bovine serum albumin (DNP-BSA) and then allophycocyanin conjugated DNP-BSA was added. A helium-neon (He-Ne) laser provided an excitation of the binding antigen-antibody complex. The resulting in fluorescence emission was measured using the sensing probe. The fluorescence intensity was proportional to the amount of allophycocyanin conjugated DNP-BSA in the sample. A linear relationship was obtained between the relative fluorescence intensity and the allergen concentration in the range 0~10 ng ml–1.
| Original language | English |
|---|---|
| Pages (from-to) | 761-765 |
| Number of pages | 5 |
| Journal | Bunseki Kagaku |
| Volume | 39 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 1990 |
| Externally published | Yes |
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Keywords
- detection of allergen
- fluoroimmunoassay
- immunoglobulin E
- optrode sensor system
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Detection of allergen using an optrode modified with immunoglobulin E immobilized membrane. / Noriyuki, Nakamura; Matsunaga, Tadashi.
In: Bunseki Kagaku, Vol. 39, No. 11, 1990, p. 761-765.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of allergen using an optrode modified with immunoglobulin E immobilized membrane
AU - Noriyuki, Nakamura
AU - Matsunaga, Tadashi
PY - 1990
Y1 - 1990
N2 - An optrode system based on a competitive binding technique for fluoroimmuoassay was developed. Immunoglobulin E (IgE) was immobilized on a membrane which covered the edge of an optical fiber. The sensor was exposed to a solution containing dinitrophenylated bovine serum albumin (DNP-BSA) and then allophycocyanin conjugated DNP-BSA was added. A helium-neon (He-Ne) laser provided an excitation of the binding antigen-antibody complex. The resulting in fluorescence emission was measured using the sensing probe. The fluorescence intensity was proportional to the amount of allophycocyanin conjugated DNP-BSA in the sample. A linear relationship was obtained between the relative fluorescence intensity and the allergen concentration in the range 0~10 ng ml–1.
AB - An optrode system based on a competitive binding technique for fluoroimmuoassay was developed. Immunoglobulin E (IgE) was immobilized on a membrane which covered the edge of an optical fiber. The sensor was exposed to a solution containing dinitrophenylated bovine serum albumin (DNP-BSA) and then allophycocyanin conjugated DNP-BSA was added. A helium-neon (He-Ne) laser provided an excitation of the binding antigen-antibody complex. The resulting in fluorescence emission was measured using the sensing probe. The fluorescence intensity was proportional to the amount of allophycocyanin conjugated DNP-BSA in the sample. A linear relationship was obtained between the relative fluorescence intensity and the allergen concentration in the range 0~10 ng ml–1.
KW - detection of allergen
KW - fluoroimmunoassay
KW - immunoglobulin E
KW - optrode sensor system
UR - http://www.scopus.com/inward/record.url?scp=85011184258&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85011184258&partnerID=8YFLogxK
U2 - 10.2116/bunsekikagaku.39.11_761
DO - 10.2116/bunsekikagaku.39.11_761
M3 - Article
VL - 39
SP - 761
EP - 765
JO - Bunseki Kagaku
JF - Bunseki Kagaku
SN - 0525-1931
IS - 11
ER -