Detection of biomolecular interaction between biotin and streptavidin on a self-assembled monolayer using magnetic nanoparticles

Atsushi Arakaki, Sho Hideshima, Takahito Nakagawa, Daisuke Niwa, Tsuyoshi Tanaka, Tadashi Matsunaga, Tetsuya Osaka

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

For developing a magnetic bioassay system, an investigation to determine the presence of a specific biomolecular interaction between biotin and streptavidin was done using magnetic nanoparticles and a silicon substrate with a self-assembled monolayer. Streptavidin was immobilized on the magnetic particles, and biotin was attached to the monolayer-modified substrate. The reaction of streptavidin-modified magnetic particles on the biotin-modified substrate was clearly observed under an optical microscope. The magnetic signals from the particles were detected using a magnetic force microscope. The results of this study demonstrate that the combination of a monolayer-modified substrate with biomolecule-modified magnetic particles is useful for detecting biomolecular interactions in medical and diagnostic analyses.

Original languageEnglish
Pages (from-to)543-546
Number of pages4
JournalBiotechnology and bioengineering
Volume88
Issue number4
DOIs
Publication statusPublished - 2004 Nov 20

Keywords

  • Biomolecular interaction
  • Biotin
  • Magnetic detection
  • Magnetic particles
  • Self-assembled monolayer
  • Streptavidin

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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