Abstract
Nanotechnology is becoming increasingly important for products used in our daily lives, such as the masses of titanium dioxide nanoparticle agglomerates (TiO2 NPs) used in the pharmaceutical industry, for cosmetic products, or for pigments. Meanwhile, a serious lack of detailed information concerning the interaction between the nanomaterials and cells limits their biological and medical applications. Sensing technology is very important for understanding these interactions. We have shown that TiO2 NPs induce heat shock protein 70B' (HSP70B') mRNA [Okuda-Shimazaki et al., 2010. Int J Mol Sci 11:2383-2392]. In the current work, sensor cells for detection of cellular responses to NPs were prepared by transfecting an HSP70B' promoter-reporter plasmid. First, to find suitable cells for detection, five different mammalian cell lines were chosen as potential sensor cells. The results showed TiO2 NP response in some cell lines, although different sensor cells had different TiO2 NP response levels, as heat shock response ability is important for the detection. Then, we studied the TiO2 NP time-course response and dose response. The results indicated that our sensor cells can detect TiO2 NP cellular responses. Our work should aid in understanding the interactions between bio-nanomaterials and cells.
| Original language | English |
|---|---|
| Pages (from-to) | 3112-3118 |
| Number of pages | 7 |
| Journal | Biotechnology and Bioengineering |
| Volume | 109 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2012 Dec |
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Keywords
- Biosensor
- Cell-materials interaction
- Cellular response
- HSP70B' promoter
- TiO nanoparticle
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
Cite this
Detection of cellular response to titanium dioxide nanoparticle agglomerates by sensor cells using heat shock protein promoter. / Chen, Peng; Kanehira, Koki; Sonezaki, Shuji; Taniguchi, Akiyoshi.
In: Biotechnology and Bioengineering, Vol. 109, No. 12, 12.2012, p. 3112-3118.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of cellular response to titanium dioxide nanoparticle agglomerates by sensor cells using heat shock protein promoter
AU - Chen, Peng
AU - Kanehira, Koki
AU - Sonezaki, Shuji
AU - Taniguchi, Akiyoshi
PY - 2012/12
Y1 - 2012/12
N2 - Nanotechnology is becoming increasingly important for products used in our daily lives, such as the masses of titanium dioxide nanoparticle agglomerates (TiO2 NPs) used in the pharmaceutical industry, for cosmetic products, or for pigments. Meanwhile, a serious lack of detailed information concerning the interaction between the nanomaterials and cells limits their biological and medical applications. Sensing technology is very important for understanding these interactions. We have shown that TiO2 NPs induce heat shock protein 70B' (HSP70B') mRNA [Okuda-Shimazaki et al., 2010. Int J Mol Sci 11:2383-2392]. In the current work, sensor cells for detection of cellular responses to NPs were prepared by transfecting an HSP70B' promoter-reporter plasmid. First, to find suitable cells for detection, five different mammalian cell lines were chosen as potential sensor cells. The results showed TiO2 NP response in some cell lines, although different sensor cells had different TiO2 NP response levels, as heat shock response ability is important for the detection. Then, we studied the TiO2 NP time-course response and dose response. The results indicated that our sensor cells can detect TiO2 NP cellular responses. Our work should aid in understanding the interactions between bio-nanomaterials and cells.
AB - Nanotechnology is becoming increasingly important for products used in our daily lives, such as the masses of titanium dioxide nanoparticle agglomerates (TiO2 NPs) used in the pharmaceutical industry, for cosmetic products, or for pigments. Meanwhile, a serious lack of detailed information concerning the interaction between the nanomaterials and cells limits their biological and medical applications. Sensing technology is very important for understanding these interactions. We have shown that TiO2 NPs induce heat shock protein 70B' (HSP70B') mRNA [Okuda-Shimazaki et al., 2010. Int J Mol Sci 11:2383-2392]. In the current work, sensor cells for detection of cellular responses to NPs were prepared by transfecting an HSP70B' promoter-reporter plasmid. First, to find suitable cells for detection, five different mammalian cell lines were chosen as potential sensor cells. The results showed TiO2 NP response in some cell lines, although different sensor cells had different TiO2 NP response levels, as heat shock response ability is important for the detection. Then, we studied the TiO2 NP time-course response and dose response. The results indicated that our sensor cells can detect TiO2 NP cellular responses. Our work should aid in understanding the interactions between bio-nanomaterials and cells.
KW - Biosensor
KW - Cell-materials interaction
KW - Cellular response
KW - HSP70B' promoter
KW - TiO nanoparticle
UR - http://www.scopus.com/inward/record.url?scp=84868128739&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84868128739&partnerID=8YFLogxK
U2 - 10.1002/bit.24583
DO - 10.1002/bit.24583
M3 - Article
C2 - 22729720
AN - SCOPUS:84868128739
VL - 109
SP - 3112
EP - 3118
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 0006-3592
IS - 12
ER -