Detection of cellular response to titanium dioxide nanoparticle agglomerates by sensor cells using heat shock protein promoter

Peng Chen, Koki Kanehira, Shuji Sonezaki, Akiyoshi Taniguchi

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Nanotechnology is becoming increasingly important for products used in our daily lives, such as the masses of titanium dioxide nanoparticle agglomerates (TiO2 NPs) used in the pharmaceutical industry, for cosmetic products, or for pigments. Meanwhile, a serious lack of detailed information concerning the interaction between the nanomaterials and cells limits their biological and medical applications. Sensing technology is very important for understanding these interactions. We have shown that TiO2 NPs induce heat shock protein 70B' (HSP70B') mRNA [Okuda-Shimazaki et al., 2010. Int J Mol Sci 11:2383-2392]. In the current work, sensor cells for detection of cellular responses to NPs were prepared by transfecting an HSP70B' promoter-reporter plasmid. First, to find suitable cells for detection, five different mammalian cell lines were chosen as potential sensor cells. The results showed TiO2 NP response in some cell lines, although different sensor cells had different TiO2 NP response levels, as heat shock response ability is important for the detection. Then, we studied the TiO2 NP time-course response and dose response. The results indicated that our sensor cells can detect TiO2 NP cellular responses. Our work should aid in understanding the interactions between bio-nanomaterials and cells.

    Original languageEnglish
    Pages (from-to)3112-3118
    Number of pages7
    JournalBiotechnology and Bioengineering
    Volume109
    Issue number12
    DOIs
    Publication statusPublished - 2012 Dec

    Fingerprint

    Heat-Shock Proteins
    Nanoparticles
    Titanium dioxide
    Proteins
    Sensors
    Nanostructured materials
    Cells
    Nanostructures
    Cosmetics
    Medical applications
    Nanotechnology
    Pigments
    Drug products
    Plasmids
    Heat-Shock Response
    Cell Line
    Drug Industry
    Messenger RNA
    Hot Temperature
    titanium dioxide

    Keywords

    • Biosensor
    • Cell-materials interaction
    • Cellular response
    • HSP70B' promoter
    • TiO nanoparticle

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology

    Cite this

    Detection of cellular response to titanium dioxide nanoparticle agglomerates by sensor cells using heat shock protein promoter. / Chen, Peng; Kanehira, Koki; Sonezaki, Shuji; Taniguchi, Akiyoshi.

    In: Biotechnology and Bioengineering, Vol. 109, No. 12, 12.2012, p. 3112-3118.

    Research output: Contribution to journalArticle

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