Detection of cellular response to titanium dioxide nanoparticle agglomerates by sensor cells using heat shock protein promoter

Peng Chen, Koki Kanehira, Shuji Sonezaki, Akiyoshi Taniguchi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Nanotechnology is becoming increasingly important for products used in our daily lives, such as the masses of titanium dioxide nanoparticle agglomerates (TiO2 NPs) used in the pharmaceutical industry, for cosmetic products, or for pigments. Meanwhile, a serious lack of detailed information concerning the interaction between the nanomaterials and cells limits their biological and medical applications. Sensing technology is very important for understanding these interactions. We have shown that TiO2 NPs induce heat shock protein 70B' (HSP70B') mRNA [Okuda-Shimazaki et al., 2010. Int J Mol Sci 11:2383-2392]. In the current work, sensor cells for detection of cellular responses to NPs were prepared by transfecting an HSP70B' promoter-reporter plasmid. First, to find suitable cells for detection, five different mammalian cell lines were chosen as potential sensor cells. The results showed TiO2 NP response in some cell lines, although different sensor cells had different TiO2 NP response levels, as heat shock response ability is important for the detection. Then, we studied the TiO2 NP time-course response and dose response. The results indicated that our sensor cells can detect TiO2 NP cellular responses. Our work should aid in understanding the interactions between bio-nanomaterials and cells.

Original languageEnglish
Pages (from-to)3112-3118
Number of pages7
JournalBiotechnology and bioengineering
Issue number12
Publication statusPublished - 2012 Dec
Externally publishedYes


  • Biosensor
  • Cell-materials interaction
  • Cellular response
  • HSP70B' promoter
  • TiO nanoparticle

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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