Detection of Defluvicoccus-related Glycogen-accumulating Organisms in Enhanced Biological Phosphorus Removal Processes

Takashi Kondo, Yoshitaka Ebie, Satoshi Tsuneda, Yuhei Inamori

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

To distinguish subgroups of Defluvicoccus-related glycogen-accumulating organisms (Defluvicoccus-related G-bacteria), new oligonucleotide probes were designed. Two of these probes, DF636 and DF827, were used successfully to detect each subgroup of Defluvicoccus-related G-bacteria. The morphological differences in clusters and cells between the DF636- and DF827-binding cells were confirmed. Interestingly, DF636-binding cells showed autofluorescence under UV light. Additionally, autofluorescence was observed in some alphaproteobacterial G-bacteria, which did not bind to any of the probes designed in this study. The results suggested that the Defluvicoccus-related G-bacteria consist of several physiologically different subspecies.

Original languageEnglish
Pages (from-to)190-195
Number of pages6
JournalMicrobes and Environments
Volume22
Issue number2
DOIs
Publication statusPublished - 2007 Jan 1

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Keywords

  • Alphaproteobacteria
  • Enhanced biological phosphorus removal (EBPR)
  • Fluorescence in situ hybridization (FISH)
  • G-bacteria
  • Genus Defluvicoccus

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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