Detection of Defluvicoccus-related Glycogen-accumulating Organisms in Enhanced Biological Phosphorus Removal Processes

Takashi Kondo, Yoshitaka Ebie, Satoshi Tsuneda, Yuhei Inamori

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    To distinguish subgroups of Defluvicoccus-related glycogen-accumulating organisms (Defluvicoccus-related G-bacteria), new oligonucleotide probes were designed. Two of these probes, DF636 and DF827, were used successfully to detect each subgroup of Defluvicoccus-related G-bacteria. The morphological differences in clusters and cells between the DF636- and DF827-binding cells were confirmed. Interestingly, DF636-binding cells showed autofluorescence under UV light. Additionally, autofluorescence was observed in some alphaproteobacterial G-bacteria, which did not bind to any of the probes designed in this study. The results suggested that the Defluvicoccus-related G-bacteria consist of several physiologically different subspecies.

    Original languageEnglish
    Pages (from-to)190-195
    Number of pages6
    JournalMicrobes and Environments
    Volume22
    Issue number2
    DOIs
    Publication statusPublished - 2007

    Fingerprint

    Defluvicoccus
    glycogen
    phosphorus
    bacterium
    bacteria
    organisms
    probe
    oligonucleotide probes
    cells
    subspecies
    ultraviolet radiation
    detection
    organism
    removal

    Keywords

    • Alphaproteobacteria
    • Enhanced biological phosphorus removal (EBPR)
    • Fluorescence in situ hybridization (FISH)
    • G-bacteria
    • Genus Defluvicoccus

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Soil Science
    • Plant Science

    Cite this

    Detection of Defluvicoccus-related Glycogen-accumulating Organisms in Enhanced Biological Phosphorus Removal Processes. / Kondo, Takashi; Ebie, Yoshitaka; Tsuneda, Satoshi; Inamori, Yuhei.

    In: Microbes and Environments, Vol. 22, No. 2, 2007, p. 190-195.

    Research output: Contribution to journalArticle

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