Detection of epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer (NSCLC) using a fully automated system with a nano-scale engineered biomagnetite

Kohei Maruyama, Haruko Takeyama, Tetsushi Mori, Keiichi Ohshima, Shun Ichiro Ogura, Toru Mochizuki, Tadashi Matsunaga

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    A fully automated system using nano-scale engineered biomagnetite was developed to detect mutations in the epidermal growth factor receptor (EGFR) gene in non-small cell lung cancer (NSCLC). Bacterial magnetic particles (BacMPs) were isolated from the magnetic bacterium Magnetospirillum magneticum AMB-1 and conjugated to streptavidin. Biotin-labeled target PCR products were then captured with the BacMPs, hybridized with the detection probe and detected by fluorescence signaling. The process was performed using a newly designed automated processor equipped with an XYZ mobile arm containing a 96-way automated pipetter, reagent dispenser and fluorescence detector. Two types of somatic mutations (in-frame deletions and point substitutions) in the EGFR gene were successfully identified within 3.5 h using this system, suggesting that this system could be used in clinical tests of EGFR gene mutations in lung cancer, and potentially other cancer, patients. Additionally, a very low mutation rate could be detected in these samples.

    Original languageEnglish
    Pages (from-to)2282-2288
    Number of pages7
    JournalBiosensors and Bioelectronics
    Volume22
    Issue number9-10
    DOIs
    Publication statusPublished - 2007 Apr 15

    Fingerprint

    erbB-1 Genes
    Epidermal Growth Factor Receptor
    Non-Small Cell Lung Carcinoma
    Genes
    Cells
    Mutation
    Magnetospirillum
    Fluorescence
    Dispensers
    Streptavidin
    Mutation Rate
    Biotin
    Lung Neoplasms
    Bacteria
    Substitution reactions
    Detectors
    Polymerase Chain Reaction
    Epidermal Growth Factor
    Neoplasms

    Keywords

    • Allele-specific oligonucleotide hybridization
    • Automated system
    • Bacterial magnetic particles (BacMPs)
    • Epidermal growth factor receptor (EGFR) mutation
    • Nano-scale engineered biomagnetite
    • Non-small cell lung cancer (NSCLC)

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Electrochemistry

    Cite this

    Detection of epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer (NSCLC) using a fully automated system with a nano-scale engineered biomagnetite. / Maruyama, Kohei; Takeyama, Haruko; Mori, Tetsushi; Ohshima, Keiichi; Ogura, Shun Ichiro; Mochizuki, Toru; Matsunaga, Tadashi.

    In: Biosensors and Bioelectronics, Vol. 22, No. 9-10, 15.04.2007, p. 2282-2288.

    Research output: Contribution to journalArticle

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    abstract = "A fully automated system using nano-scale engineered biomagnetite was developed to detect mutations in the epidermal growth factor receptor (EGFR) gene in non-small cell lung cancer (NSCLC). Bacterial magnetic particles (BacMPs) were isolated from the magnetic bacterium Magnetospirillum magneticum AMB-1 and conjugated to streptavidin. Biotin-labeled target PCR products were then captured with the BacMPs, hybridized with the detection probe and detected by fluorescence signaling. The process was performed using a newly designed automated processor equipped with an XYZ mobile arm containing a 96-way automated pipetter, reagent dispenser and fluorescence detector. Two types of somatic mutations (in-frame deletions and point substitutions) in the EGFR gene were successfully identified within 3.5 h using this system, suggesting that this system could be used in clinical tests of EGFR gene mutations in lung cancer, and potentially other cancer, patients. Additionally, a very low mutation rate could be detected in these samples.",
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