Detection of epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer (NSCLC) using a fully automated system with a nano-scale engineered biomagnetite

Kohei Maruyama, Haruko Takeyama, Tetsushi Mori, Keiichi Ohshima, Shun Ichiro Ogura, Toru Mochizuki, Tadashi Matsunaga

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A fully automated system using nano-scale engineered biomagnetite was developed to detect mutations in the epidermal growth factor receptor (EGFR) gene in non-small cell lung cancer (NSCLC). Bacterial magnetic particles (BacMPs) were isolated from the magnetic bacterium Magnetospirillum magneticum AMB-1 and conjugated to streptavidin. Biotin-labeled target PCR products were then captured with the BacMPs, hybridized with the detection probe and detected by fluorescence signaling. The process was performed using a newly designed automated processor equipped with an XYZ mobile arm containing a 96-way automated pipetter, reagent dispenser and fluorescence detector. Two types of somatic mutations (in-frame deletions and point substitutions) in the EGFR gene were successfully identified within 3.5 h using this system, suggesting that this system could be used in clinical tests of EGFR gene mutations in lung cancer, and potentially other cancer, patients. Additionally, a very low mutation rate could be detected in these samples.

Original languageEnglish
Pages (from-to)2282-2288
Number of pages7
JournalBiosensors and Bioelectronics
Volume22
Issue number9-10
DOIs
Publication statusPublished - 2007 Apr 15

Keywords

  • Allele-specific oligonucleotide hybridization
  • Automated system
  • Bacterial magnetic particles (BacMPs)
  • Epidermal growth factor receptor (EGFR) mutation
  • Nano-scale engineered biomagnetite
  • Non-small cell lung cancer (NSCLC)

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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