Detection of human leucocytes by cyclic voltammetry and its application to monitoring of allergic reaction

Noriyuki Nakamura; Ichiro Inoue; Kitajima Youji; Tadashi Matsunaga; Tomoyuki Chiba; Teruo Honda

doi:10.1016/0956-5663(91)87008-Y

Detection of human leucocytes by cyclic voltammetry and its application to monitoring of allergic reaction

Noriyuki Nakamura, Ichiro Inoue, Kitajima Youji, Tadashi Matsunaga^*, Tomoyuki Chiba, Teruo Honda

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Cyclic voltammetry was applied to the detection of human leucocytes and the monitoring of allergic reactions. A basal plane pyrolytic graphite electrode with attached leucocytes on a porous nitrocellulose membrane filter was employed as a working electrode. An anodic peak current appeared at 0·33 V versus the saturated calomel electrode (SCE) when the potential of the working electrode was scanned in the range of 0-1·0 V versus SCE. This peak current was attributed to the electrochemical oxidation of serotonin. When egg white was added to leucocytes obtained from patients who were allergic to egg, the peak current decreased owing to degranulation of leucocytes leading to serotonin release. The peak current decreased with increasing allergen concentration in the range of 5-50 μg ml^-1. Leucocytes did not respond to other allergens such as soybean, milk and dinitrophenylated bovine serum albumin (DNP-BSA).

Original language	English
Pages (from-to)	431-437
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	6
Issue number	5
DOIs	https://doi.org/10.1016/0956-5663(91)87008-Y
Publication status	Published - 1991
Externally published	Yes

Keywords

allergic reaction.
cyclic voltammetry
immunoglobulin E
leucocyte
serotonin

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Electrochemistry

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10.1016/0956-5663(91)87008-Y

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title = "Detection of human leucocytes by cyclic voltammetry and its application to monitoring of allergic reaction",

abstract = "Cyclic voltammetry was applied to the detection of human leucocytes and the monitoring of allergic reactions. A basal plane pyrolytic graphite electrode with attached leucocytes on a porous nitrocellulose membrane filter was employed as a working electrode. An anodic peak current appeared at 0·33 V versus the saturated calomel electrode (SCE) when the potential of the working electrode was scanned in the range of 0-1·0 V versus SCE. This peak current was attributed to the electrochemical oxidation of serotonin. When egg white was added to leucocytes obtained from patients who were allergic to egg, the peak current decreased owing to degranulation of leucocytes leading to serotonin release. The peak current decreased with increasing allergen concentration in the range of 5-50 μg ml-1. Leucocytes did not respond to other allergens such as soybean, milk and dinitrophenylated bovine serum albumin (DNP-BSA).",

keywords = "allergic reaction., cyclic voltammetry, immunoglobulin E, leucocyte, serotonin",

author = "Noriyuki Nakamura and Ichiro Inoue and Kitajima Youji and Tadashi Matsunaga and Tomoyuki Chiba and Teruo Honda",

year = "1991",

doi = "10.1016/0956-5663(91)87008-Y",

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T1 - Detection of human leucocytes by cyclic voltammetry and its application to monitoring of allergic reaction

AU - Nakamura, Noriyuki

AU - Inoue, Ichiro

AU - Youji, Kitajima

AU - Matsunaga, Tadashi

AU - Chiba, Tomoyuki

AU - Honda, Teruo

PY - 1991

Y1 - 1991

N2 - Cyclic voltammetry was applied to the detection of human leucocytes and the monitoring of allergic reactions. A basal plane pyrolytic graphite electrode with attached leucocytes on a porous nitrocellulose membrane filter was employed as a working electrode. An anodic peak current appeared at 0·33 V versus the saturated calomel electrode (SCE) when the potential of the working electrode was scanned in the range of 0-1·0 V versus SCE. This peak current was attributed to the electrochemical oxidation of serotonin. When egg white was added to leucocytes obtained from patients who were allergic to egg, the peak current decreased owing to degranulation of leucocytes leading to serotonin release. The peak current decreased with increasing allergen concentration in the range of 5-50 μg ml-1. Leucocytes did not respond to other allergens such as soybean, milk and dinitrophenylated bovine serum albumin (DNP-BSA).

AB - Cyclic voltammetry was applied to the detection of human leucocytes and the monitoring of allergic reactions. A basal plane pyrolytic graphite electrode with attached leucocytes on a porous nitrocellulose membrane filter was employed as a working electrode. An anodic peak current appeared at 0·33 V versus the saturated calomel electrode (SCE) when the potential of the working electrode was scanned in the range of 0-1·0 V versus SCE. This peak current was attributed to the electrochemical oxidation of serotonin. When egg white was added to leucocytes obtained from patients who were allergic to egg, the peak current decreased owing to degranulation of leucocytes leading to serotonin release. The peak current decreased with increasing allergen concentration in the range of 5-50 μg ml-1. Leucocytes did not respond to other allergens such as soybean, milk and dinitrophenylated bovine serum albumin (DNP-BSA).

KW - allergic reaction.

KW - cyclic voltammetry

KW - immunoglobulin E

KW - leucocyte

KW - serotonin

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JO - Biosensors and Bioelectronics

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IS - 5

ER -

Detection of human leucocytes by cyclic voltammetry and its application to monitoring of allergic reaction

Abstract

Keywords

ASJC Scopus subject areas

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