Detection of mismatched DNA on partially negatively charged diamond surfaces by optical and potentiometric methods

Shoma Kuga, Jung Hoon Yang, Hironori Takahashi, Kazuyuki Hirama, Takayuki Iwasaki, Hiroshi Kawarada

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The effects of surface charge density on DNA hybridization have been investigated on a mixture of hydrogen-, oxygen-, and amine-terminated diamond surfaces. A difference in the hybridization efficiencies of complementary and mismatched DNA was clearly observed by fluorescence and potentiometric observations at a particular coverage of oxygen. In the fluorescence observation, singly mismatched DNA was detected with high contrast after appropriate hybridization on the surface with 10-20% oxygen coverage. The amount of oxygen in the form of C-O- (deprotonated C-OH) producing the surface negative-charge density was estimated by X-ray photoelectron spectroscopy. Electrolyte solution gate field-effect transistors (SGFETs) were used for potentiometric observations. The signal difference (change in gate potential) on the SGFET, which was as large as ∼20 mV, was caused by the difference in the hybridization efficiencies of complementary target DNA (cDNA) and singly mismatched (1MM) target DNA with a common probe DNA immobilized on the same SGFET. The reversible change in gate potential caused by the hybridization and denaturation cycles and discriminating between the complementary and 1MM DNA targets was very stable throughout the cyclical detections. Moreover, the ratio of signals caused by hybridization of the cDNA and 1MM DNA targets with the probe DNA immobilized on the SGFET was determined to be 3:1 when hybridization had occurred (after 15 min on SGFET), as determined by real-time measurements. From the viewpoint of hybridization kinetics, the rate constant for hybridization of singly mismatched DNA was a factor of ∼3 smaller than that of cDNA on this functionalized (oxidized and aminated) diamond surface.

Original languageEnglish
Pages (from-to)13251-13263
Number of pages13
JournalJournal of the American Chemical Society
Volume130
Issue number40
DOIs
Publication statusPublished - 2008 Oct 8
Externally publishedYes

Fingerprint

Diamond
Diamonds
DNA
Complementary DNA
Gates (transistor)
Immobilized Nucleic Acids
Oxygen
DNA Probes
Fluorescence
Photoelectron Spectroscopy
Charge density
Electrolytes
Amines
Hydrogen
Observation
Denaturation
Surface charge
Time measurement
Rate constants
X ray photoelectron spectroscopy

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Detection of mismatched DNA on partially negatively charged diamond surfaces by optical and potentiometric methods. / Kuga, Shoma; Yang, Jung Hoon; Takahashi, Hironori; Hirama, Kazuyuki; Iwasaki, Takayuki; Kawarada, Hiroshi.

In: Journal of the American Chemical Society, Vol. 130, No. 40, 08.10.2008, p. 13251-13263.

Research output: Contribution to journalArticle

Kuga, Shoma ; Yang, Jung Hoon ; Takahashi, Hironori ; Hirama, Kazuyuki ; Iwasaki, Takayuki ; Kawarada, Hiroshi. / Detection of mismatched DNA on partially negatively charged diamond surfaces by optical and potentiometric methods. In: Journal of the American Chemical Society. 2008 ; Vol. 130, No. 40. pp. 13251-13263.
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