Detection of norovirus virus-like particles using a surface plasmon resonance-assisted fluoroimmunosensor optimized for quantum dot fluorescent labels

Hiroki Ashiba, Yuki Sugiyama, Xiaomin Wang, Haruko Shirato, Kyoko Higo-Moriguchi, Koki Taniguchi, Yoshimichi Ohki, Makoto Fujimaki

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    A highly sensitive biosensor to detect norovirus in environment is desired to prevent the spread of infection. In this study, we investigated a design of surface plasmon resonance (SPR)-assisted fluoroimmunosensor to increase its sensitivity and performed detection of norovirus virus-like particles (VLPs). A quantum dot fluorescent dye was employed because of its large Stokes shift. The sensor design was optimized for the CdSe-ZnS-based quantum dots. The optimal design was applied to a simple SPR-assisted fluoroimmunosensor that uses a sensor chip equipped with a V-shaped trench. Excitation efficiency of the quantum dots, degree of electric field enhancement by SPR, and intensity of autofluorescence of a substrate of the sensor chip were theoretically and experimentally evaluated to maximize the signal-to-noise ratio. As the result, an excitation wavelength of 390. nm was selected to excite SPR on an Al film of the sensor chip. The sandwich assay of norovirus VLPs was performed using the designed sensor. Minimum detectable concentration of 0.01. ng/mL, which corresponds to 100 virus-like particles included in the detection region of the V-trench, was demonstrated.

    Original languageEnglish
    JournalBiosensors and Bioelectronics
    DOIs
    Publication statusAccepted/In press - 2016 Jun 21

    Fingerprint

    Norovirus
    Quantum Dots
    Surface Plasmon Resonance
    Surface plasmon resonance
    Viruses
    Virion
    Semiconductor quantum dots
    Labels
    Sensors
    Signal-To-Noise Ratio
    Biosensing Techniques
    Fluorescent Dyes
    Biosensors
    Assays
    Signal to noise ratio
    Dyes
    Electric fields
    Infection
    Wavelength
    Substrates

    Keywords

    • Norovirus detection
    • Quantum dot
    • Sensitivity enhancement
    • Sensor design
    • Surface plasmon resonance
    • V-trench biosensor

    ASJC Scopus subject areas

    • Biotechnology
    • Biophysics
    • Biomedical Engineering
    • Electrochemistry

    Cite this

    Detection of norovirus virus-like particles using a surface plasmon resonance-assisted fluoroimmunosensor optimized for quantum dot fluorescent labels. / Ashiba, Hiroki; Sugiyama, Yuki; Wang, Xiaomin; Shirato, Haruko; Higo-Moriguchi, Kyoko; Taniguchi, Koki; Ohki, Yoshimichi; Fujimaki, Makoto.

    In: Biosensors and Bioelectronics, 21.06.2016.

    Research output: Contribution to journalArticle

    Ashiba, Hiroki ; Sugiyama, Yuki ; Wang, Xiaomin ; Shirato, Haruko ; Higo-Moriguchi, Kyoko ; Taniguchi, Koki ; Ohki, Yoshimichi ; Fujimaki, Makoto. / Detection of norovirus virus-like particles using a surface plasmon resonance-assisted fluoroimmunosensor optimized for quantum dot fluorescent labels. In: Biosensors and Bioelectronics. 2016.
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