Detection of pre-mRNA splicing in vitro by an RNA-templated fluorogenic reaction

Yasutsugu Tamura, Kazuhiro Furukawa, Rei Yoshimoto, Yuto Kawai, Minoru Yoshida, Satoshi Tsuneda, Yoshihiro Ito, Hiroshi Abe

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    RNA splicing is an important target for basic research of disease mechanisms and for drug discovery. Here, we report a new method for analysis of the in vitro RNA splicing process that produces fluorescence using a reduction-triggered fluorescence (RETF) probe. The fluorescence signal is produced only when the two probes bind side-by-side with a specific RNA target. Precursor messenger RNA and mature messenger RNA originating from the chicken δ-crystallin (CDC) gene were successfully discriminated in solution using an RETF probe with the assistance of helper oligonucleotide strands. Also, we successfully applied RETF probes to the detection of emerging mature mRNA in an in vitro splicing process.

    Original languageEnglish
    Pages (from-to)7248-7251
    Number of pages4
    JournalBioorganic and Medicinal Chemistry Letters
    Volume22
    Issue number23
    DOIs
    Publication statusPublished - 2012 Dec 1

    Fingerprint

    RNA Precursors
    Fluorescence
    RNA
    RNA Splicing
    Messenger RNA
    Crystallins
    Drug Discovery
    Centers for Disease Control and Prevention (U.S.)
    Oligonucleotides
    Chickens
    Genes
    In Vitro Techniques
    Research

    Keywords

    • Fluorescence
    • RNA
    • RNA-templated reaction
    • Splicing

    ASJC Scopus subject areas

    • Pharmaceutical Science
    • Drug Discovery
    • Organic Chemistry
    • Molecular Medicine
    • Molecular Biology
    • Clinical Biochemistry
    • Biochemistry

    Cite this

    Detection of pre-mRNA splicing in vitro by an RNA-templated fluorogenic reaction. / Tamura, Yasutsugu; Furukawa, Kazuhiro; Yoshimoto, Rei; Kawai, Yuto; Yoshida, Minoru; Tsuneda, Satoshi; Ito, Yoshihiro; Abe, Hiroshi.

    In: Bioorganic and Medicinal Chemistry Letters, Vol. 22, No. 23, 01.12.2012, p. 7248-7251.

    Research output: Contribution to journalArticle

    Tamura, Y, Furukawa, K, Yoshimoto, R, Kawai, Y, Yoshida, M, Tsuneda, S, Ito, Y & Abe, H 2012, 'Detection of pre-mRNA splicing in vitro by an RNA-templated fluorogenic reaction', Bioorganic and Medicinal Chemistry Letters, vol. 22, no. 23, pp. 7248-7251. https://doi.org/10.1016/j.bmcl.2012.09.033
    Tamura, Yasutsugu ; Furukawa, Kazuhiro ; Yoshimoto, Rei ; Kawai, Yuto ; Yoshida, Minoru ; Tsuneda, Satoshi ; Ito, Yoshihiro ; Abe, Hiroshi. / Detection of pre-mRNA splicing in vitro by an RNA-templated fluorogenic reaction. In: Bioorganic and Medicinal Chemistry Letters. 2012 ; Vol. 22, No. 23. pp. 7248-7251.
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    AU - Yoshida, Minoru

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