Abstract
Electrochemical detection of the rat basophilic leukemia (RBL-1) cells has been carried out by applying cyclic voltammetry. The detection system consists of a basal plane pyrolytic graphite electrode and a porous nitrocellulose membrane filter to trap RBL-1 cells. When the potential of the graphite electrode was run In the range of 0-1.0 V vs SCE, RBL-1 cells gave peak currents at 0.34 V vs SCE as well as 0.85 V vs SCE. There Is a linear relationship between the peak current at 0.34 V vs SCE and the cell numbers of RBL-1 In the range of (0.4-2.0) X 105 cells. The peak current of RBL-1 cells was attributed to serotonin. When dinitrophenylated bovine serum albumin (DNP-BSA) as a model allergen was added to RBL-1 cells sensitized with anti-DNP IgE, the peak current decreased because of the degranulation of RBL-1 cells leading to serotonin release. On the other hand, RBL-1 cells sensitized with anti-DNP IgE did not respond to egg white, pollens, house dust, and milk.
Original language | English |
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Pages (from-to) | 2471-2474 |
Number of pages | 4 |
Journal | Analytical Chemistry |
Volume | 61 |
Issue number | 22 |
DOIs | |
Publication status | Published - 1989 |
Externally published | Yes |
ASJC Scopus subject areas
- Analytical Chemistry