Detection of rat basophilic leukemia by cyclic voltammetry for monitoring allergic reaction

Tadashi Matsunaga; Akinori Shigematsu; Noriyuki Nakamura

doi:10.1021/ac00197a004

Detection of rat basophilic leukemia by cyclic voltammetry for monitoring allergic reaction

Tadashi Matsunaga, Akinori Shigematsu, Noriyuki Nakamura

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Electrochemical detection of the rat basophilic leukemia (RBL-1) cells has been carried out by applying cyclic voltammetry. The detection system consists of a basal plane pyrolytic graphite electrode and a porous nitrocellulose membrane filter to trap RBL-1 cells. When the potential of the graphite electrode was run In the range of 0-1.0 V vs SCE, RBL-1 cells gave peak currents at 0.34 V vs SCE as well as 0.85 V vs SCE. There Is a linear relationship between the peak current at 0.34 V vs SCE and the cell numbers of RBL-1 In the range of (0.4-2.0) X 10⁵ cells. The peak current of RBL-1 cells was attributed to serotonin. When dinitrophenylated bovine serum albumin (DNP-BSA) as a model allergen was added to RBL-1 cells sensitized with anti-DNP IgE, the peak current decreased because of the degranulation of RBL-1 cells leading to serotonin release. On the other hand, RBL-1 cells sensitized with anti-DNP IgE did not respond to egg white, pollens, house dust, and milk.

Original language	English
Pages (from-to)	2471-2474
Number of pages	4
Journal	Analytical Chemistry
Volume	61
Issue number	22
DOIs	https://doi.org/10.1021/ac00197a004
Publication status	Published - 1989
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

Access to Document

10.1021/ac00197a004

Fingerprint Dive into the research topics of 'Detection of rat basophilic leukemia by cyclic voltammetry for monitoring allergic reaction'. Together they form a unique fingerprint.

Cite this

@article{5a67232f81804127864747adec6a4d62,

title = "Detection of rat basophilic leukemia by cyclic voltammetry for monitoring allergic reaction",

abstract = "Electrochemical detection of the rat basophilic leukemia (RBL-1) cells has been carried out by applying cyclic voltammetry. The detection system consists of a basal plane pyrolytic graphite electrode and a porous nitrocellulose membrane filter to trap RBL-1 cells. When the potential of the graphite electrode was run In the range of 0-1.0 V vs SCE, RBL-1 cells gave peak currents at 0.34 V vs SCE as well as 0.85 V vs SCE. There Is a linear relationship between the peak current at 0.34 V vs SCE and the cell numbers of RBL-1 In the range of (0.4-2.0) X 105 cells. The peak current of RBL-1 cells was attributed to serotonin. When dinitrophenylated bovine serum albumin (DNP-BSA) as a model allergen was added to RBL-1 cells sensitized with anti-DNP IgE, the peak current decreased because of the degranulation of RBL-1 cells leading to serotonin release. On the other hand, RBL-1 cells sensitized with anti-DNP IgE did not respond to egg white, pollens, house dust, and milk.",

author = "Tadashi Matsunaga and Akinori Shigematsu and Noriyuki Nakamura",

year = "1989",

doi = "10.1021/ac00197a004",

language = "English",

volume = "61",

pages = "2471--2474",

journal = "Industrial And Engineering Chemistry Analytical Edition",

issn = "0003-2700",

publisher = "American Chemical Society",

number = "22",

}

TY - JOUR

T1 - Detection of rat basophilic leukemia by cyclic voltammetry for monitoring allergic reaction

AU - Matsunaga, Tadashi

AU - Shigematsu, Akinori

AU - Nakamura, Noriyuki

PY - 1989

Y1 - 1989

N2 - Electrochemical detection of the rat basophilic leukemia (RBL-1) cells has been carried out by applying cyclic voltammetry. The detection system consists of a basal plane pyrolytic graphite electrode and a porous nitrocellulose membrane filter to trap RBL-1 cells. When the potential of the graphite electrode was run In the range of 0-1.0 V vs SCE, RBL-1 cells gave peak currents at 0.34 V vs SCE as well as 0.85 V vs SCE. There Is a linear relationship between the peak current at 0.34 V vs SCE and the cell numbers of RBL-1 In the range of (0.4-2.0) X 105 cells. The peak current of RBL-1 cells was attributed to serotonin. When dinitrophenylated bovine serum albumin (DNP-BSA) as a model allergen was added to RBL-1 cells sensitized with anti-DNP IgE, the peak current decreased because of the degranulation of RBL-1 cells leading to serotonin release. On the other hand, RBL-1 cells sensitized with anti-DNP IgE did not respond to egg white, pollens, house dust, and milk.

AB - Electrochemical detection of the rat basophilic leukemia (RBL-1) cells has been carried out by applying cyclic voltammetry. The detection system consists of a basal plane pyrolytic graphite electrode and a porous nitrocellulose membrane filter to trap RBL-1 cells. When the potential of the graphite electrode was run In the range of 0-1.0 V vs SCE, RBL-1 cells gave peak currents at 0.34 V vs SCE as well as 0.85 V vs SCE. There Is a linear relationship between the peak current at 0.34 V vs SCE and the cell numbers of RBL-1 In the range of (0.4-2.0) X 105 cells. The peak current of RBL-1 cells was attributed to serotonin. When dinitrophenylated bovine serum albumin (DNP-BSA) as a model allergen was added to RBL-1 cells sensitized with anti-DNP IgE, the peak current decreased because of the degranulation of RBL-1 cells leading to serotonin release. On the other hand, RBL-1 cells sensitized with anti-DNP IgE did not respond to egg white, pollens, house dust, and milk.

UR - http://www.scopus.com/inward/record.url?scp=0024788739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024788739&partnerID=8YFLogxK

U2 - 10.1021/ac00197a004

DO - 10.1021/ac00197a004

M3 - Article

C2 - 2479300

AN - SCOPUS:0024788739

VL - 61

SP - 2471

EP - 2474

JO - Industrial And Engineering Chemistry Analytical Edition

JF - Industrial And Engineering Chemistry Analytical Edition

SN - 0003-2700

IS - 22

ER -

Detection of rat basophilic leukemia by cyclic voltammetry for monitoring allergic reaction

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this