Detection of rat basophilic leukemia by cyclic voltammetry for monitoring allergic reaction

Tadashi Matsunaga; Akinori Shigematsu; Noriyuki Nakamura

doi:10.1021/ac00197a004

Detection of rat basophilic leukemia by cyclic voltammetry for monitoring allergic reaction

Tadashi Matsunaga, Akinori Shigematsu, Noriyuki Nakamura

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Abstract

Electrochemical detection of the rat basophilic leukemia (RBL-1) cells has been carried out by applying cyclic voltammetry. The detection system consists of a basal plane pyrolytic graphite electrode and a porous nitrocellulose membrane filter to trap RBL-1 cells. When the potential of the graphite electrode was run In the range of 0-1.0 V vs SCE, RBL-1 cells gave peak currents at 0.34 V vs SCE as well as 0.85 V vs SCE. There Is a linear relationship between the peak current at 0.34 V vs SCE and the cell numbers of RBL-1 In the range of (0.4-2.0) X 10⁵ cells. The peak current of RBL-1 cells was attributed to serotonin. When dinitrophenylated bovine serum albumin (DNP-BSA) as a model allergen was added to RBL-1 cells sensitized with anti-DNP IgE, the peak current decreased because of the degranulation of RBL-1 cells leading to serotonin release. On the other hand, RBL-1 cells sensitized with anti-DNP IgE did not respond to egg white, pollens, house dust, and milk.

Original language	English
Pages (from-to)	2471-2474
Number of pages	4
Journal	Analytical Chemistry
Volume	61
Issue number	22
DOIs	https://doi.org/10.1021/ac00197a004
Publication status	Published - 1989
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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Matsunaga, T., Shigematsu, A., & Nakamura, N. (1989). Detection of rat basophilic leukemia by cyclic voltammetry for monitoring allergic reaction. Analytical Chemistry, 61(22), 2471-2474. https://doi.org/10.1021/ac00197a004

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title = "Detection of rat basophilic leukemia by cyclic voltammetry for monitoring allergic reaction",

abstract = "Electrochemical detection of the rat basophilic leukemia (RBL-1) cells has been carried out by applying cyclic voltammetry. The detection system consists of a basal plane pyrolytic graphite electrode and a porous nitrocellulose membrane filter to trap RBL-1 cells. When the potential of the graphite electrode was run In the range of 0-1.0 V vs SCE, RBL-1 cells gave peak currents at 0.34 V vs SCE as well as 0.85 V vs SCE. There Is a linear relationship between the peak current at 0.34 V vs SCE and the cell numbers of RBL-1 In the range of (0.4-2.0) X 105 cells. The peak current of RBL-1 cells was attributed to serotonin. When dinitrophenylated bovine serum albumin (DNP-BSA) as a model allergen was added to RBL-1 cells sensitized with anti-DNP IgE, the peak current decreased because of the degranulation of RBL-1 cells leading to serotonin release. On the other hand, RBL-1 cells sensitized with anti-DNP IgE did not respond to egg white, pollens, house dust, and milk.",

author = "Tadashi Matsunaga and Akinori Shigematsu and Noriyuki Nakamura",

year = "1989",

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N2 - Electrochemical detection of the rat basophilic leukemia (RBL-1) cells has been carried out by applying cyclic voltammetry. The detection system consists of a basal plane pyrolytic graphite electrode and a porous nitrocellulose membrane filter to trap RBL-1 cells. When the potential of the graphite electrode was run In the range of 0-1.0 V vs SCE, RBL-1 cells gave peak currents at 0.34 V vs SCE as well as 0.85 V vs SCE. There Is a linear relationship between the peak current at 0.34 V vs SCE and the cell numbers of RBL-1 In the range of (0.4-2.0) X 105 cells. The peak current of RBL-1 cells was attributed to serotonin. When dinitrophenylated bovine serum albumin (DNP-BSA) as a model allergen was added to RBL-1 cells sensitized with anti-DNP IgE, the peak current decreased because of the degranulation of RBL-1 cells leading to serotonin release. On the other hand, RBL-1 cells sensitized with anti-DNP IgE did not respond to egg white, pollens, house dust, and milk.

AB - Electrochemical detection of the rat basophilic leukemia (RBL-1) cells has been carried out by applying cyclic voltammetry. The detection system consists of a basal plane pyrolytic graphite electrode and a porous nitrocellulose membrane filter to trap RBL-1 cells. When the potential of the graphite electrode was run In the range of 0-1.0 V vs SCE, RBL-1 cells gave peak currents at 0.34 V vs SCE as well as 0.85 V vs SCE. There Is a linear relationship between the peak current at 0.34 V vs SCE and the cell numbers of RBL-1 In the range of (0.4-2.0) X 105 cells. The peak current of RBL-1 cells was attributed to serotonin. When dinitrophenylated bovine serum albumin (DNP-BSA) as a model allergen was added to RBL-1 cells sensitized with anti-DNP IgE, the peak current decreased because of the degranulation of RBL-1 cells leading to serotonin release. On the other hand, RBL-1 cells sensitized with anti-DNP IgE did not respond to egg white, pollens, house dust, and milk.

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