Detection of tetanus-induced effects in linearly lined-up micropatterned neuronal networks: Application of a multi-electrode array chip combined with agarose microstructures

Ikurou Suzuki, Kenji Yasuda

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

One of the best approaches to understanding the mechanism of information acquisition and storage is to characterize the plasticity of network activity by monitoring and stimulating individual neurons in a topologically defined network and doing this for extended periods of time. We therefore previously developed an on-chip multi-electrode array (MEA) system combined with an array of agarose microchambers (AMCs). It is possible to record the firing at multiple cells simultaneously for long term and topographically control the cells position and their connections. In our present study, we demonstrated the effect of tetanic stimulation in a linearly lined-up patterned network on the AMC/MEA chip. We detected reproducible activity changes that were induced by tetanic stimulation and saw that these changes were maintained for 6-24 h. The results show the advantage of our AMC/MEA cultivation and measurements methods and suggest they will be useful for investigating the long-term plasticity depending on network topology and size.

Original languageEnglish
Pages (from-to)470-475
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume356
Issue number2
DOIs
Publication statusPublished - 2007 May 4
Externally publishedYes

Fingerprint

Tetanus
Sepharose
Electrodes
Microstructure
Plasticity
Information Storage and Retrieval
Neurons
Topology
Monitoring

Keywords

  • Agarose microchamber
  • Hippocampal cell
  • Long-term measurements
  • Multi-electrode array (MEA)
  • Neuronal network
  • Patterning
  • Photothermal etching
  • Plasticity
  • Reproducibility
  • Tetanic stimulation
  • Tetanus
  • Topology

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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abstract = "One of the best approaches to understanding the mechanism of information acquisition and storage is to characterize the plasticity of network activity by monitoring and stimulating individual neurons in a topologically defined network and doing this for extended periods of time. We therefore previously developed an on-chip multi-electrode array (MEA) system combined with an array of agarose microchambers (AMCs). It is possible to record the firing at multiple cells simultaneously for long term and topographically control the cells position and their connections. In our present study, we demonstrated the effect of tetanic stimulation in a linearly lined-up patterned network on the AMC/MEA chip. We detected reproducible activity changes that were induced by tetanic stimulation and saw that these changes were maintained for 6-24 h. The results show the advantage of our AMC/MEA cultivation and measurements methods and suggest they will be useful for investigating the long-term plasticity depending on network topology and size.",
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