Detection of tumor marker in blood serum using antibody-modified field effect transistor with optimized BSA blocking

Sho Hideshima, Ryosuke Sato, Sayaka Inoue, Shigeki Kuroiwa, Tetsuya Osaka

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

This paper demonstrates that antibody-modified field effect transistors (FETs), which were treated with bovine serum albumin (BSA) blocking, had quantitative capability for tumor marker detection in blood serum. Antibody-modified surface covered with 0.1% BSA effectively minimized nonspecific adsorption of blood proteins. Tumor markers with their isoelectric points of around 5 generated strong signals in body environmental pH (pH 7.4) in proteins with isoelectric points ranging from 3 to 10. Detection of a tumor marker alpha-fetoprotein in blood serum at required level for clinical diagnosis (10 ng/mL) was achieved. The obtained results suggest that the fabricated FETs be expected to practical application in medical fields for direct detection of multi-tumor markers from blood.

Original languageEnglish
Pages (from-to)146-150
Number of pages5
JournalSensors and Actuators, B: Chemical
Volume161
Issue number1
DOIs
Publication statusPublished - 2012 Jan 3

Fingerprint

blood serum
Tumor Biomarkers
Bovine Serum Albumin
Field effect transistors
antibodies
albumins
Antibodies
serums
markers
Blood
tumors
field effect transistors
blood
proteins
Proteins
alpha-Fetoproteins
Blood Proteins
Adsorption
adsorption

Keywords

  • Blocking
  • Blood serum
  • Field effect transistor
  • Nonspecific adsorption
  • Tumor marker

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Detection of tumor marker in blood serum using antibody-modified field effect transistor with optimized BSA blocking. / Hideshima, Sho; Sato, Ryosuke; Inoue, Sayaka; Kuroiwa, Shigeki; Osaka, Tetsuya.

In: Sensors and Actuators, B: Chemical, Vol. 161, No. 1, 03.01.2012, p. 146-150.

Research output: Contribution to journalArticle

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