TY - JOUR
T1 - Detection of tumor marker in blood serum using antibody-modified field effect transistor with optimized BSA blocking
AU - Hideshima, Sho
AU - Sato, Ryosuke
AU - Inoue, Sayaka
AU - Kuroiwa, Shigeki
AU - Osaka, Tetsuya
N1 - Funding Information:
This work was partly supported by Grant-in-Aid for Specially Promoted Research (2002006) “Establishment of Electrochemical Device Engineering”, and by Global COE program “Center for Practical Chemical Wisdom”, both from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan .
PY - 2012/1/3
Y1 - 2012/1/3
N2 - This paper demonstrates that antibody-modified field effect transistors (FETs), which were treated with bovine serum albumin (BSA) blocking, had quantitative capability for tumor marker detection in blood serum. Antibody-modified surface covered with 0.1% BSA effectively minimized nonspecific adsorption of blood proteins. Tumor markers with their isoelectric points of around 5 generated strong signals in body environmental pH (pH 7.4) in proteins with isoelectric points ranging from 3 to 10. Detection of a tumor marker alpha-fetoprotein in blood serum at required level for clinical diagnosis (10 ng/mL) was achieved. The obtained results suggest that the fabricated FETs be expected to practical application in medical fields for direct detection of multi-tumor markers from blood.
AB - This paper demonstrates that antibody-modified field effect transistors (FETs), which were treated with bovine serum albumin (BSA) blocking, had quantitative capability for tumor marker detection in blood serum. Antibody-modified surface covered with 0.1% BSA effectively minimized nonspecific adsorption of blood proteins. Tumor markers with their isoelectric points of around 5 generated strong signals in body environmental pH (pH 7.4) in proteins with isoelectric points ranging from 3 to 10. Detection of a tumor marker alpha-fetoprotein in blood serum at required level for clinical diagnosis (10 ng/mL) was achieved. The obtained results suggest that the fabricated FETs be expected to practical application in medical fields for direct detection of multi-tumor markers from blood.
KW - Blocking
KW - Blood serum
KW - Field effect transistor
KW - Nonspecific adsorption
KW - Tumor marker
UR - http://www.scopus.com/inward/record.url?scp=84856235955&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84856235955&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2011.10.001
DO - 10.1016/j.snb.2011.10.001
M3 - Article
AN - SCOPUS:84856235955
SN - 0925-4005
VL - 161
SP - 146
EP - 150
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
IS - 1
ER -