Determination of cholesterol with a laboratory-built chemiluminescence system

Akiyoshi Taniguchi, Yuzuru Hayashi, Hidetaka Yuki

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Chemiluminescence measurement by a laboratory-built computer system equipped with an automated injection device is applied to a cholesterol enzymatic assay. The hydrogen peroxide produced by cholesterol esterase and oxidase is mixed rapidly with horseradish peroxidase-luminol solution (pH 10.5) and the light emission data are processed by a Fourier-transform digital filter to produce peak-height measurements. A blood serum volume of 0.2 μl and an observation time of 0.5 s are required.

Original languageEnglish
Pages (from-to)95-100
Number of pages6
JournalAnalytica Chimica Acta
Volume188
Issue numberC
DOIs
Publication statusPublished - 1986
Externally publishedYes

Fingerprint

Cholesterol Oxidase
Luminescent Measurements
Sterol Esterase
Luminol
Chemiluminescence
computer system
Computer Systems
Enzyme Assays
Fourier Analysis
Horseradish Peroxidase
Luminescence
Blood Volume
hydrogen peroxide
Hydrogen Peroxide
Fourier transform
serum
blood
Cholesterol
Observation
assay

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Determination of cholesterol with a laboratory-built chemiluminescence system. / Taniguchi, Akiyoshi; Hayashi, Yuzuru; Yuki, Hidetaka.

In: Analytica Chimica Acta, Vol. 188, No. C, 1986, p. 95-100.

Research output: Contribution to journalArticle

Taniguchi, Akiyoshi ; Hayashi, Yuzuru ; Yuki, Hidetaka. / Determination of cholesterol with a laboratory-built chemiluminescence system. In: Analytica Chimica Acta. 1986 ; Vol. 188, No. C. pp. 95-100.
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