Determination of cholesterol with a laboratory-built chemiluminescence system

Akiyoshi Taniguchi*, Yuzuru Hayashi, Hidetaka Yuki

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Chemiluminescence measurement by a laboratory-built computer system equipped with an automated injection device is applied to a cholesterol enzymatic assay. The hydrogen peroxide produced by cholesterol esterase and oxidase is mixed rapidly with horseradish peroxidase-luminol solution (pH 10.5) and the light emission data are processed by a Fourier-transform digital filter to produce peak-height measurements. A blood serum volume of 0.2 μl and an observation time of 0.5 s are required.

Original languageEnglish
Pages (from-to)95-100
Number of pages6
JournalAnalytica Chimica Acta
Volume188
Issue numberC
DOIs
Publication statusPublished - 1986
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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