Abstract
Background: Although the systemic inflammatory condition can be confirmed in cancer patients, the pathophysiological importance of reactive oxygen species (ROS) produced by neutrophils has not yet been defined. Patients and Methods: Twenty-one patients with inoperable, chemoresistant and radioresistant cancer were enrolled in this study. At least 4 weeks prior to sampling, the patients were free from antitumor treatments. Control samples were also obtained from a healthy donor (39-year-old male). Peripheral blood samples were set 150 μl each on the 2 ml tube with 50 μl Mebiol Gel, and the production of ROS from neutrophils was detected by luminol-dependent chemiluminescence (LmCL) in a kinetic mode at 30-minute intervals for 2.5 hours with a luminometer at 37°C. Results: Each point, peak value and sum of values of LmCL in the patient group was statistically higher than those in the healthy donor. There were no differences in LmCL according to performance status (PS), type of cancer, age, or gender in cancer patients. Conclusion: Our findings suggested that ROS produced by neutrophils universally reflects the systemic inflammatory condition in cancer patients.
| Original language | English |
|---|---|
| Pages (from-to) | 565-570 |
| Number of pages | 6 |
| Journal | Anticancer Research |
| Volume | 32 |
| Issue number | 2 |
| Publication status | Published - 2012 Feb |
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Keywords
- Cancer
- Inflammation
- Luminol-dependent chemiluminescence
- Mebiol Gel
- Neutrophil
- Oxidative stress
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Determination of chronic inflammatory states in cancer patients using assay of reactive oxygen species production by neutrophils. / Suzuki, Yoko; Ohno, Satoshi; Okuyama, Ryuji; Aruga, Atsushi; Yamamoto, Masakazu; Miura, Shigeki; Yoshioka, Hiroshi; Mori, Yuichi; Suzuki, Katsuhiko.
In: Anticancer Research, Vol. 32, No. 2, 02.2012, p. 565-570.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determination of chronic inflammatory states in cancer patients using assay of reactive oxygen species production by neutrophils
AU - Suzuki, Yoko
AU - Ohno, Satoshi
AU - Okuyama, Ryuji
AU - Aruga, Atsushi
AU - Yamamoto, Masakazu
AU - Miura, Shigeki
AU - Yoshioka, Hiroshi
AU - Mori, Yuichi
AU - Suzuki, Katsuhiko
PY - 2012/2
Y1 - 2012/2
N2 - Background: Although the systemic inflammatory condition can be confirmed in cancer patients, the pathophysiological importance of reactive oxygen species (ROS) produced by neutrophils has not yet been defined. Patients and Methods: Twenty-one patients with inoperable, chemoresistant and radioresistant cancer were enrolled in this study. At least 4 weeks prior to sampling, the patients were free from antitumor treatments. Control samples were also obtained from a healthy donor (39-year-old male). Peripheral blood samples were set 150 μl each on the 2 ml tube with 50 μl Mebiol Gel, and the production of ROS from neutrophils was detected by luminol-dependent chemiluminescence (LmCL) in a kinetic mode at 30-minute intervals for 2.5 hours with a luminometer at 37°C. Results: Each point, peak value and sum of values of LmCL in the patient group was statistically higher than those in the healthy donor. There were no differences in LmCL according to performance status (PS), type of cancer, age, or gender in cancer patients. Conclusion: Our findings suggested that ROS produced by neutrophils universally reflects the systemic inflammatory condition in cancer patients.
AB - Background: Although the systemic inflammatory condition can be confirmed in cancer patients, the pathophysiological importance of reactive oxygen species (ROS) produced by neutrophils has not yet been defined. Patients and Methods: Twenty-one patients with inoperable, chemoresistant and radioresistant cancer were enrolled in this study. At least 4 weeks prior to sampling, the patients were free from antitumor treatments. Control samples were also obtained from a healthy donor (39-year-old male). Peripheral blood samples were set 150 μl each on the 2 ml tube with 50 μl Mebiol Gel, and the production of ROS from neutrophils was detected by luminol-dependent chemiluminescence (LmCL) in a kinetic mode at 30-minute intervals for 2.5 hours with a luminometer at 37°C. Results: Each point, peak value and sum of values of LmCL in the patient group was statistically higher than those in the healthy donor. There were no differences in LmCL according to performance status (PS), type of cancer, age, or gender in cancer patients. Conclusion: Our findings suggested that ROS produced by neutrophils universally reflects the systemic inflammatory condition in cancer patients.
KW - Cancer
KW - Inflammation
KW - Luminol-dependent chemiluminescence
KW - Mebiol Gel
KW - Neutrophil
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=84863126969&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863126969&partnerID=8YFLogxK
M3 - Article
C2 - 22287746
AN - SCOPUS:84863126969
VL - 32
SP - 565
EP - 570
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 2
ER -