Determination of chronic inflammatory states in cancer patients using assay of reactive oxygen species production by neutrophils

Yoko Suzuki, Satoshi Ohno, Ryuji Okuyama, Atsushi Aruga, Masakazu Yamamoto, Shigeki Miura, Hiroshi Yoshioka, Yuichi Mori, Katsuhiko Suzuki

Research output: Contribution to journalArticle

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Abstract

Background: Although the systemic inflammatory condition can be confirmed in cancer patients, the pathophysiological importance of reactive oxygen species (ROS) produced by neutrophils has not yet been defined. Patients and Methods: Twenty-one patients with inoperable, chemoresistant and radioresistant cancer were enrolled in this study. At least 4 weeks prior to sampling, the patients were free from antitumor treatments. Control samples were also obtained from a healthy donor (39-year-old male). Peripheral blood samples were set 150 μl each on the 2 ml tube with 50 μl Mebiol Gel, and the production of ROS from neutrophils was detected by luminol-dependent chemiluminescence (LmCL) in a kinetic mode at 30-minute intervals for 2.5 hours with a luminometer at 37°C. Results: Each point, peak value and sum of values of LmCL in the patient group was statistically higher than those in the healthy donor. There were no differences in LmCL according to performance status (PS), type of cancer, age, or gender in cancer patients. Conclusion: Our findings suggested that ROS produced by neutrophils universally reflects the systemic inflammatory condition in cancer patients.

Original languageEnglish
Pages (from-to)565-570
Number of pages6
JournalAnticancer Research
Volume32
Issue number2
Publication statusPublished - 2012 Feb

Fingerprint

Reactive Oxygen Species
Neutrophils
Luminol
Luminescence
Neoplasms
Tissue Donors
Gels

Keywords

  • Cancer
  • Inflammation
  • Luminol-dependent chemiluminescence
  • Mebiol Gel
  • Neutrophil
  • Oxidative stress

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Determination of chronic inflammatory states in cancer patients using assay of reactive oxygen species production by neutrophils. / Suzuki, Yoko; Ohno, Satoshi; Okuyama, Ryuji; Aruga, Atsushi; Yamamoto, Masakazu; Miura, Shigeki; Yoshioka, Hiroshi; Mori, Yuichi; Suzuki, Katsuhiko.

In: Anticancer Research, Vol. 32, No. 2, 02.2012, p. 565-570.

Research output: Contribution to journalArticle

Suzuki, Y, Ohno, S, Okuyama, R, Aruga, A, Yamamoto, M, Miura, S, Yoshioka, H, Mori, Y & Suzuki, K 2012, 'Determination of chronic inflammatory states in cancer patients using assay of reactive oxygen species production by neutrophils', Anticancer Research, vol. 32, no. 2, pp. 565-570.
Suzuki, Yoko ; Ohno, Satoshi ; Okuyama, Ryuji ; Aruga, Atsushi ; Yamamoto, Masakazu ; Miura, Shigeki ; Yoshioka, Hiroshi ; Mori, Yuichi ; Suzuki, Katsuhiko. / Determination of chronic inflammatory states in cancer patients using assay of reactive oxygen species production by neutrophils. In: Anticancer Research. 2012 ; Vol. 32, No. 2. pp. 565-570.
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