Determination of protein concentration with two-dimensional (2D) Photon burst diagrams using microfluidic channel

Nan Jing; Chin B. Su; Chao Kai Chou; Mien Chie Hung; Jun Kameoka

Determination of protein concentration with two-dimensional (2D) Photon burst diagrams using microfluidic channel

Nan Jing, Chin B. Su, Chao Kai Chou, Mien Chie Hung, Jun Kameoka

Research output: Contribution to conference › Paper › peer-review

Abstract

The determination of target protein concentration has been demonstrated by a 2D photon burst (photon counts vs. burst width) analysis technique and microfluidic channel device. Since this technique is based on counting individual molecule events, we can effectively estimate the concentration of the specific target molecule among other molecules in a sample solution in a simpler, faster and more accurate way comparing to the conventional ensemble measurement techniques. It is also possible to study protein-protein interaction with only one fluorescent label. This could find potential applications in clinical diagnostics and signal pathway study in the future.

Original language	English
Pages	865-867
Number of pages	3
Publication status	Published - 2008
Externally published	Yes
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 2008 Oct 12 → 2008 Oct 16

Conference

Conference	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	08/10/12 → 08/10/16

Keywords

Microchannel
Polyclonal antibody
Single molecule detection

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Determination of protein concentration with two-dimensional (2D) Photon burst diagrams using microfluidic channel. / Jing, Nan; Su, Chin B.; Chou, Chao Kai et al.

2008. 865-867 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

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keywords = "Microchannel, Polyclonal antibody, Single molecule detection",

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year = "2008",

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pages = "865--867",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

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T1 - Determination of protein concentration with two-dimensional (2D) Photon burst diagrams using microfluidic channel

AU - Jing, Nan

AU - Su, Chin B.

AU - Chou, Chao Kai

AU - Hung, Mien Chie

AU - Kameoka, Jun

PY - 2008

Y1 - 2008

N2 - The determination of target protein concentration has been demonstrated by a 2D photon burst (photon counts vs. burst width) analysis technique and microfluidic channel device. Since this technique is based on counting individual molecule events, we can effectively estimate the concentration of the specific target molecule among other molecules in a sample solution in a simpler, faster and more accurate way comparing to the conventional ensemble measurement techniques. It is also possible to study protein-protein interaction with only one fluorescent label. This could find potential applications in clinical diagnostics and signal pathway study in the future.

AB - The determination of target protein concentration has been demonstrated by a 2D photon burst (photon counts vs. burst width) analysis technique and microfluidic channel device. Since this technique is based on counting individual molecule events, we can effectively estimate the concentration of the specific target molecule among other molecules in a sample solution in a simpler, faster and more accurate way comparing to the conventional ensemble measurement techniques. It is also possible to study protein-protein interaction with only one fluorescent label. This could find potential applications in clinical diagnostics and signal pathway study in the future.

KW - Microchannel

KW - Polyclonal antibody

KW - Single molecule detection

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M3 - Paper

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SP - 865

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T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Determination of protein concentration with two-dimensional (2D) Photon burst diagrams using microfluidic channel

Abstract

Conference

Keywords

ASJC Scopus subject areas

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