Abstract
An open-tubular enzyme reactor and an ammonium ion selective electrode using nonactin were prepared and connected sequentially to determine serum urea by flow injection method. Urease was immobilized on the inner surface of aminosilanized silicone rubber tubing. The effects of sample size, pH value and flow rate of the carrier solution on the enzyme reactor were investigated. The following practical standard conditions for the measurement were decided; sample size: 20 μl, pH value: 6.5, and flow rate of the carrier solution: 1.0ml min–1. The calibration range for urea concentration was from 2.0 up to 300 mM. The relative standard deviations for 10 replicate measurements of a urea standardand a control serum were 0.65% and 1.19%, respectively. Long-term stability of the urease reactor was improved by coating thin film of cellulose acetate over the whole immobilized enzyme layer. The measurement system was applied to serum samples from 20 healthy women. Good correlation between the present method and colorimetry was observed.
| Original language | English |
|---|---|
| Pages (from-to) | 755-760 |
| Number of pages | 6 |
| Journal | BUNSEKI KAGAKU |
| Volume | 39 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 1990 Jan 1 |
| Externally published | Yes |
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Keywords
- ammonium ion selective electrode
- FIA
- open-tubular immobilized urease reactor
- serum urea determination
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Determination of serum urea using an open-tubular immobilized enzyme reactor. / Kenji, Yasuda; Yoshinori, Takata.
In: BUNSEKI KAGAKU, Vol. 39, No. 11, 01.01.1990, p. 755-760.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determination of serum urea using an open-tubular immobilized enzyme reactor
AU - Kenji, Yasuda
AU - Yoshinori, Takata
PY - 1990/1/1
Y1 - 1990/1/1
N2 - An open-tubular enzyme reactor and an ammonium ion selective electrode using nonactin were prepared and connected sequentially to determine serum urea by flow injection method. Urease was immobilized on the inner surface of aminosilanized silicone rubber tubing. The effects of sample size, pH value and flow rate of the carrier solution on the enzyme reactor were investigated. The following practical standard conditions for the measurement were decided; sample size: 20 μl, pH value: 6.5, and flow rate of the carrier solution: 1.0ml min–1. The calibration range for urea concentration was from 2.0 up to 300 mM. The relative standard deviations for 10 replicate measurements of a urea standardand a control serum were 0.65% and 1.19%, respectively. Long-term stability of the urease reactor was improved by coating thin film of cellulose acetate over the whole immobilized enzyme layer. The measurement system was applied to serum samples from 20 healthy women. Good correlation between the present method and colorimetry was observed.
AB - An open-tubular enzyme reactor and an ammonium ion selective electrode using nonactin were prepared and connected sequentially to determine serum urea by flow injection method. Urease was immobilized on the inner surface of aminosilanized silicone rubber tubing. The effects of sample size, pH value and flow rate of the carrier solution on the enzyme reactor were investigated. The following practical standard conditions for the measurement were decided; sample size: 20 μl, pH value: 6.5, and flow rate of the carrier solution: 1.0ml min–1. The calibration range for urea concentration was from 2.0 up to 300 mM. The relative standard deviations for 10 replicate measurements of a urea standardand a control serum were 0.65% and 1.19%, respectively. Long-term stability of the urease reactor was improved by coating thin film of cellulose acetate over the whole immobilized enzyme layer. The measurement system was applied to serum samples from 20 healthy women. Good correlation between the present method and colorimetry was observed.
KW - ammonium ion selective electrode
KW - FIA
KW - open-tubular immobilized urease reactor
KW - serum urea determination
UR - http://www.scopus.com/inward/record.url?scp=85011185416&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85011185416&partnerID=8YFLogxK
U2 - 10.2116/bunsekikagaku.39.11_755
DO - 10.2116/bunsekikagaku.39.11_755
M3 - Article
AN - SCOPUS:85011185416
VL - 39
SP - 755
EP - 760
JO - Bunseki Kagaku
JF - Bunseki Kagaku
SN - 0525-1931
IS - 11
ER -