Determination of toluene in brain of freely moving mice using solid-phase microextraction technique

Daisuke Nakajima, Tin-Tin-Win-Shwe, Masaki Kakeyama, Hidekazu Fujimaki, Sumio Goto

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

For the purpose of measuring the pharmacokinetics of inhaled toluene in the brain of mice, we developed a method for the direct detection of toluene by gas chromatography/mass spectrometry (GC/MS). The method uses a solid-phase microextraction (SPME) fiber inserted into the mouse' hippocampus (CA1) through a cannula fixed onto the animal. BALB/c mice were exposed to 0, 0.9, 9, 50 and 90 ppm toluene for 30 min. The toluene level detected near the hippocampus by this method after exposure to 0.9 ppm toluene in air showed no significant difference from the level found in the non-exposure control group; however, the toluene level increased significantly after exposure to concentrations of 9, 50 and 90 ppm. These increases were concentration-dependent. In addition, the pharmacokinetics of toluene in the brain of mice exposed to 50 ppm toluene showed that the toluene level decreased rapidly after the exposure, and returned to control levels after 60 min. This study describes the method which has successfully detected toluene levels in the brain of conscious, free-moving mice for the first time.

Original languageEnglish
Pages (from-to)615-618
Number of pages4
JournalNeuroToxicology
Volume27
Issue number4
DOIs
Publication statusPublished - 2006 Jul 1
Externally publishedYes

Keywords

  • Brain
  • Mice
  • SPME
  • Toluene

ASJC Scopus subject areas

  • Neuroscience(all)
  • Toxicology

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