Abstract
For the purpose of measuring the pharmacokinetics of inhaled toluene in the brain of mice, we developed a method for the direct detection of toluene by gas chromatography/mass spectrometry (GC/MS). The method uses a solid-phase microextraction (SPME) fiber inserted into the mouse' hippocampus (CA1) through a cannula fixed onto the animal. BALB/c mice were exposed to 0, 0.9, 9, 50 and 90 ppm toluene for 30 min. The toluene level detected near the hippocampus by this method after exposure to 0.9 ppm toluene in air showed no significant difference from the level found in the non-exposure control group; however, the toluene level increased significantly after exposure to concentrations of 9, 50 and 90 ppm. These increases were concentration-dependent. In addition, the pharmacokinetics of toluene in the brain of mice exposed to 50 ppm toluene showed that the toluene level decreased rapidly after the exposure, and returned to control levels after 60 min. This study describes the method which has successfully detected toluene levels in the brain of conscious, free-moving mice for the first time.
| Original language | English |
|---|---|
| Pages (from-to) | 615-618 |
| Number of pages | 4 |
| Journal | NeuroToxicology |
| Volume | 27 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2006 Jul |
| Externally published | Yes |
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Keywords
- Brain
- Mice
- SPME
- Toluene
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
- Neuroscience(all)
- Toxicology
Cite this
Determination of toluene in brain of freely moving mice using solid-phase microextraction technique. / Nakajima, Daisuke; Tin-Tin-Win-Shwe, ; Kakeyama, Masaki; Fujimaki, Hidekazu; Goto, Sumio.
In: NeuroToxicology, Vol. 27, No. 4, 07.2006, p. 615-618.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determination of toluene in brain of freely moving mice using solid-phase microextraction technique
AU - Nakajima, Daisuke
AU - Tin-Tin-Win-Shwe,
AU - Kakeyama, Masaki
AU - Fujimaki, Hidekazu
AU - Goto, Sumio
PY - 2006/7
Y1 - 2006/7
N2 - For the purpose of measuring the pharmacokinetics of inhaled toluene in the brain of mice, we developed a method for the direct detection of toluene by gas chromatography/mass spectrometry (GC/MS). The method uses a solid-phase microextraction (SPME) fiber inserted into the mouse' hippocampus (CA1) through a cannula fixed onto the animal. BALB/c mice were exposed to 0, 0.9, 9, 50 and 90 ppm toluene for 30 min. The toluene level detected near the hippocampus by this method after exposure to 0.9 ppm toluene in air showed no significant difference from the level found in the non-exposure control group; however, the toluene level increased significantly after exposure to concentrations of 9, 50 and 90 ppm. These increases were concentration-dependent. In addition, the pharmacokinetics of toluene in the brain of mice exposed to 50 ppm toluene showed that the toluene level decreased rapidly after the exposure, and returned to control levels after 60 min. This study describes the method which has successfully detected toluene levels in the brain of conscious, free-moving mice for the first time.
AB - For the purpose of measuring the pharmacokinetics of inhaled toluene in the brain of mice, we developed a method for the direct detection of toluene by gas chromatography/mass spectrometry (GC/MS). The method uses a solid-phase microextraction (SPME) fiber inserted into the mouse' hippocampus (CA1) through a cannula fixed onto the animal. BALB/c mice were exposed to 0, 0.9, 9, 50 and 90 ppm toluene for 30 min. The toluene level detected near the hippocampus by this method after exposure to 0.9 ppm toluene in air showed no significant difference from the level found in the non-exposure control group; however, the toluene level increased significantly after exposure to concentrations of 9, 50 and 90 ppm. These increases were concentration-dependent. In addition, the pharmacokinetics of toluene in the brain of mice exposed to 50 ppm toluene showed that the toluene level decreased rapidly after the exposure, and returned to control levels after 60 min. This study describes the method which has successfully detected toluene levels in the brain of conscious, free-moving mice for the first time.
KW - Brain
KW - Mice
KW - SPME
KW - Toluene
UR - http://www.scopus.com/inward/record.url?scp=33744970711&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33744970711&partnerID=8YFLogxK
U2 - 10.1016/j.neuro.2005.12.006
DO - 10.1016/j.neuro.2005.12.006
M3 - Article
C2 - 16457888
AN - SCOPUS:33744970711
VL - 27
SP - 615
EP - 618
JO - NeuroToxicology
JF - NeuroToxicology
SN - 0161-813X
IS - 4
ER -