Determination of trace levels of fatty acid metal salts by high-performance liquid chromatography with fluorescence prelabeling

Katsumi Hayashi, Jiro Kawase, Koichi Yoshimura, Katsutoshi Ara, Kazuro Tsuji

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

A simple method is described for picomole determinations of fatty acid metal salts. Fatty acid salts are directly labeled with 4-bromomethyl-7-methoxycoumarin in the presence of excess ethylenediaminetetraacetic acid tripotassium salt without any solvent extractions. The fluorescence derivatives of fatty acids are separated by reverse-phase high-performance liquid chromatography followed by fluorometric detection. The response of each fatty acid (C8-C18) calcium salt is linear from 1 to 50 μg/ml of samples. The detection limit is about 7 pmol. Good recoveries are obtained for the calcium salts of myrystic acid and soap (C8-C18, C18:1,2). The new method is successfully applied to the study on biodegradation of fatty acids in river water.

Original languageEnglish
Pages (from-to)314-320
Number of pages7
JournalAnalytical Biochemistry
Volume136
Issue number2
DOIs
Publication statusPublished - 1984 Feb 1
Externally publishedYes

Keywords

  • HPLC
  • fatty acid
  • fluorescence detection
  • metal salt
  • microdetermination
  • prederivatization

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Determination of trace levels of fatty acid metal salts by high-performance liquid chromatography with fluorescence prelabeling'. Together they form a unique fingerprint.

Cite this