Development and application of homologous radioimmunoassay for newt prolactin

Kouhei Matsuda, Kazutoshi Yamamoto, Sakae Kikuyama

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    A specific and sensitive homologous radioimmunoassay (RIA) for newt (Cynops pyrrhogaster) prolactin (PRL) was developed. PRL isolated fromnewt pituitary glands was used for generating antiserum in a rabbit, for radioiodination, and for the standard. Several dilutions of plasma and pituitary homogenate of newts yielded dose-response curves which were parallel to the standard curve. Plasma from hypophysectomized newts showed the least amount of cross-reaction. Pituitary homogenates of other species of urodeles such as least amount of cross-reaction. Pituitary homogenates of other species of urodeles such as Ambystoma mexicanum and Onychodactylus japonicus gave inhibition curves which were parallel to the standard curve. Purified PRLs of anurans such as Rana catesbeiana and Bufo japonicus gave inhibition curves which did not parallel the standard. Bovine PRL and ovine PRL showed no inhibition of binding even at relatively high doses in this RIA. The RIA was applied to the determination of plasma and pituitary PRL levels in the adult newts treated with dopamine agonist (bromocriptine) and/or antagonist (pimozide). Pimozide enhanced PRL levels and bromocriptine antagonized it, while pituitary PRL levels were not appreciably changed.

    Original languageEnglish
    Pages (from-to)83-88
    Number of pages6
    JournalGeneral and Comparative Endocrinology
    Volume79
    Issue number1
    DOIs
    Publication statusPublished - 1990

    ASJC Scopus subject areas

    • Endocrinology

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