Development of a bench-top extra-cleanroom for DNA amplification

Hirokazu Takahashi; Takahiro Satoh; Hiroko Kanahara; Yuji Kubota; Tamaki Hirose; Hiroyuki Yamazaki; Kimiko Yamamoto; Yoshiko Okamura; Taketo Suzuki; Toshiro Kobori

doi:10.2144/000114433

Development of a bench-top extra-cleanroom for DNA amplification

Hirokazu Takahashi^*, Takahiro Satoh, Hiroko Kanahara, Yuji Kubota, Tamaki Hirose, Hiroyuki Yamazaki, Kimiko Yamamoto, Yoshiko Okamura, Taketo Suzuki, Toshiro Kobori

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Prevention of airborne contamination has become an important factor in biotechnology; however, conventional laminar-airflow cabinets (LAF-cabinets) are no longer sufficient as a countermeasure against nano-sized airborne contaminants in the laboratory. Here we present a bench-top extra-cleanroom classified as ISO-1 that can prevent contamination from airborne nanoparticles. This bench-top extra-cleanroom consists of a novel clean-zone-creating system that is equipped with nanofibrous, nonwoven filters. In addition, the cleanroom is also equipped with an ionizer to prevent plasticware from collecting dust by electrostatic charge attraction. This combination of features allows the cleanroom to prevent DNA contamination derived from airborne nanoparticles. Our extra-cleanroom with ionizer could be useful in various areas of biotechnology that are easily affected by airborne contaminants.

Original language	English
Pages (from-to)	42-46
Number of pages	5
Journal	BioTechniques
Volume	61
Issue number	1
DOIs	https://doi.org/10.2144/000114433
Publication status	Published - 2016 Jul
Externally published	Yes

Keywords

Airborne nanoparticle contamination
Bench-top extra-cleanroom
Random RNA primers
Whole-genome amplification
ø29 DNA polymerase

ASJC Scopus subject areas

Biotechnology
Biochemistry, Genetics and Molecular Biology(all)

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10.2144/000114433

Cite this

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title = "Development of a bench-top extra-cleanroom for DNA amplification",

abstract = "Prevention of airborne contamination has become an important factor in biotechnology; however, conventional laminar-airflow cabinets (LAF-cabinets) are no longer sufficient as a countermeasure against nano-sized airborne contaminants in the laboratory. Here we present a bench-top extra-cleanroom classified as ISO-1 that can prevent contamination from airborne nanoparticles. This bench-top extra-cleanroom consists of a novel clean-zone-creating system that is equipped with nanofibrous, nonwoven filters. In addition, the cleanroom is also equipped with an ionizer to prevent plasticware from collecting dust by electrostatic charge attraction. This combination of features allows the cleanroom to prevent DNA contamination derived from airborne nanoparticles. Our extra-cleanroom with ionizer could be useful in various areas of biotechnology that are easily affected by airborne contaminants.",

keywords = "Airborne nanoparticle contamination, Bench-top extra-cleanroom, Random RNA primers, Whole-genome amplification, {\o}29 DNA polymerase",

author = "Hirokazu Takahashi and Takahiro Satoh and Hiroko Kanahara and Yuji Kubota and Tamaki Hirose and Hiroyuki Yamazaki and Kimiko Yamamoto and Yoshiko Okamura and Taketo Suzuki and Toshiro Kobori",

note = "Funding Information: The authors thank J. Wakayama and Y. Magariyama for helpful discussions, and A. Matsumoto and K. Tsukada for excellent technical assistance. This work was partially supported by grants from the National Agriculture and Food Research Organization, the Japan Science and Technology Agency, CREST, Japan, and the Takeda Science Foundation. Publisher Copyright: {\textcopyright} 2016, Eaton Publishing Company. All rights reserved.",

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doi = "10.2144/000114433",

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AU - Takahashi, Hirokazu

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AU - Yamazaki, Hiroyuki

AU - Yamamoto, Kimiko

AU - Okamura, Yoshiko

AU - Suzuki, Taketo

AU - Kobori, Toshiro

N1 - Funding Information: The authors thank J. Wakayama and Y. Magariyama for helpful discussions, and A. Matsumoto and K. Tsukada for excellent technical assistance. This work was partially supported by grants from the National Agriculture and Food Research Organization, the Japan Science and Technology Agency, CREST, Japan, and the Takeda Science Foundation. Publisher Copyright: © 2016, Eaton Publishing Company. All rights reserved.

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Development of a bench-top extra-cleanroom for DNA amplification

Abstract

Keywords

ASJC Scopus subject areas

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