Development of a dielectrophoresis-assisted surface plasmon resonance fluorescence biosensor for detection of bacteria

Chiaki Kuroda, Ryota Iizuka, Yoshimichi Ohki, Makoto Fujimaki

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    To detect biological substances such as bacteria speedily and accurately, a dielectrophoresis-assisted surface plasmon resonance (SPR) fluorescence biosensor is being developed. Using Escherichia coli as a target organism, an appropriate voltage frequency to collect E. coli cells on indium tin oxide quadrupole electrodes by dielectrophoresis is analyzed. Then, E. coli is stained with 4$,6-diamidino-2-phenylindole (DAPI). To clearly detect fluorescence signals from DAPI-stained E. coli cells, the sensor is optimized so that we can excite SPR on Al electrodes by illuminating 405 nm photons. As a result, the number of fluorescence signals is increased on the electrodes by the application of a low-frequency voltage. This indicates that E. coli cells with a lower permittivity than the surrounding water are collected by negative dielectrophoresis onto the electrodes where the electric field strength is lowest.

    Original languageEnglish
    Article number057001
    JournalJapanese Journal of Applied Physics
    Volume57
    Issue number5
    DOIs
    Publication statusPublished - 2018 May 1

    Fingerprint

    Surface plasmon resonance
    resonance fluorescence
    Electrophoresis
    bioinstrumentation
    surface plasmon resonance
    Biosensors
    Escherichia coli
    bacteria
    Bacteria
    Fluorescence
    electrodes
    Electrodes
    cells
    fluorescence
    electric field strength
    electric potential
    Escherichia
    organisms
    illuminating
    indium oxides

    ASJC Scopus subject areas

    • Engineering(all)
    • Physics and Astronomy(all)

    Cite this

    Development of a dielectrophoresis-assisted surface plasmon resonance fluorescence biosensor for detection of bacteria. / Kuroda, Chiaki; Iizuka, Ryota; Ohki, Yoshimichi; Fujimaki, Makoto.

    In: Japanese Journal of Applied Physics, Vol. 57, No. 5, 057001, 01.05.2018.

    Research output: Contribution to journalArticle

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