Development of a non-protein and lipid medium adopted cell line for biopharmaceutical recombinant protein expression

Tetsuji Sasaki, Akiyoshi Taniguchi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Recently, many biopharmaceuticals have been developed such as cytokines, growth factors, and antibodies. These recombinant proteins are mostly expressed by CHO cells. However, the culture medium of CHO cells requires the addition of serum, which can contain unknown biological substances such as viruses, or requires the addition of expensive growth factors. To avoid the risks of biological ingredients and to decrease the cost of biopharmaceutical production, we developed a non-protein and lipid medium adopted (NPLAd) CHO cell line using the adapted culture method. Our results indicated that autocrine EGF production and insulin addition are essential for NPLAd CHO cell growth. However, the rate of cell proliferation of NPLAd CHO cells was decreased compared with original CHO-K1 cells. The proliferation of NPLAd CHO cells was improved by GM3 addition, suggesting increased signaling efficiency of autocrine factors. No difference was found in the growth rate between original CHO-K1 and NPLAd CHO cells supplemented with insulin and GM3. The productivity of recombinant protein in NPLAd CHO cells was verified using secreting luciferase reporter system. As a result, luciferase activity in NPLAd CHO cells showed more than three times higher than in the original CHOK1 cells. The results suggested that this cell line could be useful for biopharmaceutical recombinant protein.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalOpen Biotechnology Journal
Volume7
Issue number1
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Recombinant proteins
CHO Cells
Recombinant Proteins
Lipids
Cells
Cell Line
Insulin
Luciferases
Intercellular Signaling Peptides and Proteins
Autocrine Communication
Cell proliferation
Cell growth
Growth
Viruses
Epidermal Growth Factor
Antibodies
Culture Media
Productivity
Cell Proliferation
Cytokines

Keywords

  • Adapted culture method
  • Biopharmaceutical
  • CHO cells
  • EGF
  • GM3
  • Serum-free

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Biophysics
  • Bioengineering

Cite this

@article{5199c873ca114f6996a677a95b557bdb,
title = "Development of a non-protein and lipid medium adopted cell line for biopharmaceutical recombinant protein expression",
abstract = "Recently, many biopharmaceuticals have been developed such as cytokines, growth factors, and antibodies. These recombinant proteins are mostly expressed by CHO cells. However, the culture medium of CHO cells requires the addition of serum, which can contain unknown biological substances such as viruses, or requires the addition of expensive growth factors. To avoid the risks of biological ingredients and to decrease the cost of biopharmaceutical production, we developed a non-protein and lipid medium adopted (NPLAd) CHO cell line using the adapted culture method. Our results indicated that autocrine EGF production and insulin addition are essential for NPLAd CHO cell growth. However, the rate of cell proliferation of NPLAd CHO cells was decreased compared with original CHO-K1 cells. The proliferation of NPLAd CHO cells was improved by GM3 addition, suggesting increased signaling efficiency of autocrine factors. No difference was found in the growth rate between original CHO-K1 and NPLAd CHO cells supplemented with insulin and GM3. The productivity of recombinant protein in NPLAd CHO cells was verified using secreting luciferase reporter system. As a result, luciferase activity in NPLAd CHO cells showed more than three times higher than in the original CHOK1 cells. The results suggested that this cell line could be useful for biopharmaceutical recombinant protein.",
keywords = "Adapted culture method, Biopharmaceutical, CHO cells, EGF, GM3, Serum-free",
author = "Tetsuji Sasaki and Akiyoshi Taniguchi",
year = "2013",
doi = "10.2174/1874070701307010001",
language = "English",
volume = "7",
pages = "1--6",
journal = "Open Biotechnology Journal",
issn = "1874-0707",
publisher = "Bentham Science Publishers B.V.",
number = "1",

}

TY - JOUR

T1 - Development of a non-protein and lipid medium adopted cell line for biopharmaceutical recombinant protein expression

AU - Sasaki, Tetsuji

AU - Taniguchi, Akiyoshi

PY - 2013

Y1 - 2013

N2 - Recently, many biopharmaceuticals have been developed such as cytokines, growth factors, and antibodies. These recombinant proteins are mostly expressed by CHO cells. However, the culture medium of CHO cells requires the addition of serum, which can contain unknown biological substances such as viruses, or requires the addition of expensive growth factors. To avoid the risks of biological ingredients and to decrease the cost of biopharmaceutical production, we developed a non-protein and lipid medium adopted (NPLAd) CHO cell line using the adapted culture method. Our results indicated that autocrine EGF production and insulin addition are essential for NPLAd CHO cell growth. However, the rate of cell proliferation of NPLAd CHO cells was decreased compared with original CHO-K1 cells. The proliferation of NPLAd CHO cells was improved by GM3 addition, suggesting increased signaling efficiency of autocrine factors. No difference was found in the growth rate between original CHO-K1 and NPLAd CHO cells supplemented with insulin and GM3. The productivity of recombinant protein in NPLAd CHO cells was verified using secreting luciferase reporter system. As a result, luciferase activity in NPLAd CHO cells showed more than three times higher than in the original CHOK1 cells. The results suggested that this cell line could be useful for biopharmaceutical recombinant protein.

AB - Recently, many biopharmaceuticals have been developed such as cytokines, growth factors, and antibodies. These recombinant proteins are mostly expressed by CHO cells. However, the culture medium of CHO cells requires the addition of serum, which can contain unknown biological substances such as viruses, or requires the addition of expensive growth factors. To avoid the risks of biological ingredients and to decrease the cost of biopharmaceutical production, we developed a non-protein and lipid medium adopted (NPLAd) CHO cell line using the adapted culture method. Our results indicated that autocrine EGF production and insulin addition are essential for NPLAd CHO cell growth. However, the rate of cell proliferation of NPLAd CHO cells was decreased compared with original CHO-K1 cells. The proliferation of NPLAd CHO cells was improved by GM3 addition, suggesting increased signaling efficiency of autocrine factors. No difference was found in the growth rate between original CHO-K1 and NPLAd CHO cells supplemented with insulin and GM3. The productivity of recombinant protein in NPLAd CHO cells was verified using secreting luciferase reporter system. As a result, luciferase activity in NPLAd CHO cells showed more than three times higher than in the original CHOK1 cells. The results suggested that this cell line could be useful for biopharmaceutical recombinant protein.

KW - Adapted culture method

KW - Biopharmaceutical

KW - CHO cells

KW - EGF

KW - GM3

KW - Serum-free

UR - http://www.scopus.com/inward/record.url?scp=84884193276&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884193276&partnerID=8YFLogxK

U2 - 10.2174/1874070701307010001

DO - 10.2174/1874070701307010001

M3 - Article

AN - SCOPUS:84884193276

VL - 7

SP - 1

EP - 6

JO - Open Biotechnology Journal

JF - Open Biotechnology Journal

SN - 1874-0707

IS - 1

ER -