Abstract
Magnetic particles are useful for simple and efficient nucleic acid extraction. To achieve fully automated nucleic acid extraction and purification using magnetic particles, a new method for operating magnetic particles, Magtration Technology, was developed. In this method, magnetic separation is performed in a specially designed disposable tip. This enables high recovery of magnetic particles with high reproducibility. The features of this technology are (i) a simple mechanism for process control and (ii) flexible software to enable adaptation to commercially available reagents. Automated instruments based on Magtration Technology were developed and used for nucleic acid extraction. Total DNA, total RNA and plasmids were purified by Magtration Technology at an efficiency comparable to that of manual methods.
| Original language | English |
|---|---|
| Pages (from-to) | 500-503 |
| Number of pages | 4 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 91 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2001 |
| Externally published | Yes |
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Keywords
- Automation
- Beads
- Magnetic separation
- Nucleic acid
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
Cite this
Development of a novel method for operating magnetic particles, magtration technology, and its use for automating nucleic acid purification. / Obata, Kimimichi; Segawa, Osamu; Yakabe, Mitsuru; Ishida, Yoshikazu; Kuroita, Toshihiro; Ikeda, Katsunori; Kawakami, Bunsei; Kawamura, Yoshihisa; Yohda, Masafumi; Matsunaga, Tadashi; Tajima, Hideji.
In: Journal of Bioscience and Bioengineering, Vol. 91, No. 5, 2001, p. 500-503.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of a novel method for operating magnetic particles, magtration technology, and its use for automating nucleic acid purification
AU - Obata, Kimimichi
AU - Segawa, Osamu
AU - Yakabe, Mitsuru
AU - Ishida, Yoshikazu
AU - Kuroita, Toshihiro
AU - Ikeda, Katsunori
AU - Kawakami, Bunsei
AU - Kawamura, Yoshihisa
AU - Yohda, Masafumi
AU - Matsunaga, Tadashi
AU - Tajima, Hideji
PY - 2001
Y1 - 2001
N2 - Magnetic particles are useful for simple and efficient nucleic acid extraction. To achieve fully automated nucleic acid extraction and purification using magnetic particles, a new method for operating magnetic particles, Magtration Technology, was developed. In this method, magnetic separation is performed in a specially designed disposable tip. This enables high recovery of magnetic particles with high reproducibility. The features of this technology are (i) a simple mechanism for process control and (ii) flexible software to enable adaptation to commercially available reagents. Automated instruments based on Magtration Technology were developed and used for nucleic acid extraction. Total DNA, total RNA and plasmids were purified by Magtration Technology at an efficiency comparable to that of manual methods.
AB - Magnetic particles are useful for simple and efficient nucleic acid extraction. To achieve fully automated nucleic acid extraction and purification using magnetic particles, a new method for operating magnetic particles, Magtration Technology, was developed. In this method, magnetic separation is performed in a specially designed disposable tip. This enables high recovery of magnetic particles with high reproducibility. The features of this technology are (i) a simple mechanism for process control and (ii) flexible software to enable adaptation to commercially available reagents. Automated instruments based on Magtration Technology were developed and used for nucleic acid extraction. Total DNA, total RNA and plasmids were purified by Magtration Technology at an efficiency comparable to that of manual methods.
KW - Automation
KW - Beads
KW - Magnetic separation
KW - Nucleic acid
UR - http://www.scopus.com/inward/record.url?scp=0034967394&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034967394&partnerID=8YFLogxK
U2 - 10.1016/S1389-1723(01)80280-2
DO - 10.1016/S1389-1723(01)80280-2
M3 - Article
C2 - 16233029
AN - SCOPUS:0034967394
VL - 91
SP - 500
EP - 503
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 5
ER -