Development of a novel method for operating magnetic particles, magtration technology, and its use for automating nucleic acid purification

Kimimichi Obata, Osamu Segawa, Mitsuru Yakabe, Yoshikazu Ishida, Toshihiro Kuroita, Katsunori Ikeda, Bunsei Kawakami, Yoshihisa Kawamura, Masafumi Yohda, Tadashi Matsunaga, Hideji Tajima

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Magnetic particles are useful for simple and efficient nucleic acid extraction. To achieve fully automated nucleic acid extraction and purification using magnetic particles, a new method for operating magnetic particles, Magtration Technology, was developed. In this method, magnetic separation is performed in a specially designed disposable tip. This enables high recovery of magnetic particles with high reproducibility. The features of this technology are (i) a simple mechanism for process control and (ii) flexible software to enable adaptation to commercially available reagents. Automated instruments based on Magtration Technology were developed and used for nucleic acid extraction. Total DNA, total RNA and plasmids were purified by Magtration Technology at an efficiency comparable to that of manual methods.

Original languageEnglish
Pages (from-to)500-503
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume91
Issue number5
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Nucleic acids
Nucleic Acids
Purification
Technology
Magnetic separation
RNA
Process control
Plasmids
DNA
Software
Recovery

Keywords

  • Automation
  • Beads
  • Magnetic separation
  • Nucleic acid

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Development of a novel method for operating magnetic particles, magtration technology, and its use for automating nucleic acid purification. / Obata, Kimimichi; Segawa, Osamu; Yakabe, Mitsuru; Ishida, Yoshikazu; Kuroita, Toshihiro; Ikeda, Katsunori; Kawakami, Bunsei; Kawamura, Yoshihisa; Yohda, Masafumi; Matsunaga, Tadashi; Tajima, Hideji.

In: Journal of Bioscience and Bioengineering, Vol. 91, No. 5, 2001, p. 500-503.

Research output: Contribution to journalArticle

Obata, K, Segawa, O, Yakabe, M, Ishida, Y, Kuroita, T, Ikeda, K, Kawakami, B, Kawamura, Y, Yohda, M, Matsunaga, T & Tajima, H 2001, 'Development of a novel method for operating magnetic particles, magtration technology, and its use for automating nucleic acid purification', Journal of Bioscience and Bioengineering, vol. 91, no. 5, pp. 500-503. https://doi.org/10.1016/S1389-1723(01)80280-2
Obata, Kimimichi ; Segawa, Osamu ; Yakabe, Mitsuru ; Ishida, Yoshikazu ; Kuroita, Toshihiro ; Ikeda, Katsunori ; Kawakami, Bunsei ; Kawamura, Yoshihisa ; Yohda, Masafumi ; Matsunaga, Tadashi ; Tajima, Hideji. / Development of a novel method for operating magnetic particles, magtration technology, and its use for automating nucleic acid purification. In: Journal of Bioscience and Bioengineering. 2001 ; Vol. 91, No. 5. pp. 500-503.
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