Development of a novel method for operating magnetic particles, magtration technology, and its use for automating nucleic acid purification

Kimimichi Obata; Osamu Segawa; Mitsuru Yakabe; Yoshikazu Ishida; Toshihiro Kuroita; Katsunori Ikeda; Bunsei Kawakami; Yoshihisa Kawamura; Masafumi Yohda; Tadashi Matsunaga; Hideji Tajima

doi:10.1016/S1389-1723(01)80280-2

Development of a novel method for operating magnetic particles, magtration technology, and its use for automating nucleic acid purification

Kimimichi Obata, Osamu Segawa, Mitsuru Yakabe, Yoshikazu Ishida, Toshihiro Kuroita, Katsunori Ikeda, Bunsei Kawakami, Yoshihisa Kawamura, Masafumi Yohda, Tadashi Matsunaga, Hideji Tajima

Research output: Contribution to journal › Article

52 Citations (Scopus)

Abstract

Magnetic particles are useful for simple and efficient nucleic acid extraction. To achieve fully automated nucleic acid extraction and purification using magnetic particles, a new method for operating magnetic particles, Magtration Technology, was developed. In this method, magnetic separation is performed in a specially designed disposable tip. This enables high recovery of magnetic particles with high reproducibility. The features of this technology are (i) a simple mechanism for process control and (ii) flexible software to enable adaptation to commercially available reagents. Automated instruments based on Magtration Technology were developed and used for nucleic acid extraction. Total DNA, total RNA and plasmids were purified by Magtration Technology at an efficiency comparable to that of manual methods.

Original language	English
Pages (from-to)	500-503
Number of pages	4
Journal	Journal of Bioscience and Bioengineering
Volume	91
Issue number	5
DOIs	https://doi.org/10.1016/S1389-1723(01)80280-2
Publication status	Published - 2001
Externally published	Yes

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Keywords

Automation
Beads
Magnetic separation
Nucleic acid

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Cite this

Obata, K., Segawa, O., Yakabe, M., Ishida, Y., Kuroita, T., Ikeda, K., Kawakami, B., Kawamura, Y., Yohda, M., Matsunaga, T., & Tajima, H. (2001). Development of a novel method for operating magnetic particles, magtration technology, and its use for automating nucleic acid purification. Journal of Bioscience and Bioengineering, 91(5), 500-503. https://doi.org/10.1016/S1389-1723(01)80280-2

Development of a novel method for operating magnetic particles, magtration technology, and its use for automating nucleic acid purification. / Obata, Kimimichi; Segawa, Osamu; Yakabe, Mitsuru; Ishida, Yoshikazu; Kuroita, Toshihiro; Ikeda, Katsunori; Kawakami, Bunsei; Kawamura, Yoshihisa; Yohda, Masafumi; Matsunaga, Tadashi; Tajima, Hideji.

In: Journal of Bioscience and Bioengineering, Vol. 91, No. 5, 2001, p. 500-503.

Research output: Contribution to journal › Article

Obata, K, Segawa, O, Yakabe, M, Ishida, Y, Kuroita, T, Ikeda, K, Kawakami, B, Kawamura, Y, Yohda, M, Matsunaga, T & Tajima, H 2001, 'Development of a novel method for operating magnetic particles, magtration technology, and its use for automating nucleic acid purification', Journal of Bioscience and Bioengineering, vol. 91, no. 5, pp. 500-503. https://doi.org/10.1016/S1389-1723(01)80280-2

Obata, Kimimichi ; Segawa, Osamu ; Yakabe, Mitsuru ; Ishida, Yoshikazu ; Kuroita, Toshihiro ; Ikeda, Katsunori ; Kawakami, Bunsei ; Kawamura, Yoshihisa ; Yohda, Masafumi ; Matsunaga, Tadashi ; Tajima, Hideji. / Development of a novel method for operating magnetic particles, magtration technology, and its use for automating nucleic acid purification. In: Journal of Bioscience and Bioengineering. 2001 ; Vol. 91, No. 5. pp. 500-503.

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10.1016/S1389-1723(01)80280-2